Ischemia that causes stroke induces inflammation of brain cells and apoptosis and as a result, it influences much on the functional part of a man. The needle electrode electrical stimulation (NEES) that combines acupuncture of oriental medicine with electric therapy of western medicine relieves inflammation of cells and has effect on regrowth of nerve tissues. This study was conducted to verify the influence of NEES on the occurrence of c-Fos of cerebrum after applying NEES to the meridian point, Zusanli (ST 36) of a rats with induced ischemia. Global ischemia was induced by using ligation method on common carotid artery of male Sprague Dawley (SD) rats. The ligation was maintained for 5 minutes and then suture was removed for blood reperfusion. After inducing global ischemia, NEES was done to the left and right meridian points of Joksamri of a rat for 30 minutes after 12 hours, 24 hours, and 48 hours. The findings were as follows. 1. In the result of immunohistochemical method, the number of c-Fos immune response cells significantly decreased (P<.05) in NEES group than the control group (GI) that did not get NEES. 2. In the result of western blotting, the occurrence of c-Fos after 24 hours from the inducement of ischemia significantly decreased (P<.05) in NEES group than the control group (GI) that did not get NEES. Therefore, as the effect of NEES was shown highest after 24 hours from the ischemia, it is suspected that NEES would take important role in early treatment after cerebral stroke.
김기현, 이윤호. 침자극이 백서의 맥박수와 혈압에 미치는 영향. 대한침구학회지. 1986;(1):3-52.
김선국, 이용만, 방선웅 등. 벤자틴 페니실린에 의해 유도된 국소 발작간 간질양 파형이 백서 대뇌에서 c-jun 단백질 발현 양상에 미치는 영향. 대한신경과학회지. 2000;18(5):617-623.
김성원, 김병기, 이정숙 등. Effects of needle electrode electrical stimulation on rat brain by ischemia-induced common carotid artery occlusion. 한국실험동물학회. 2008;24(4):593-599.
김경식, 임종국, 김정유. 십선혈침자가 자연발증고혈압 백서의 혈압에 미치는 영향. 대한침구학회지. 1984;1(1):97-104.
김용석, 김창완, 허영범 등. 전침자극이 SHR 흰쥐 대뇌의 NADPH-diaphorase와 Neuropeptide Y 신경세포에 미치는 영향. 대한침구학회지. 1999;16(4):283-291.
서영아, 송형근, 나창수 등. 족삼리의 전기침자극에 대한 개구반사 크기의 변화. 대한소아치과학회지. 1998;25(4):789-96.
신권성. 족삼리 침자가 동통억제에 미치는 영향. 원광대학교 대학원, 석사학위논문, 1998.
이상룡, 강성길, 박동석. 자락요법이 혈압 및 혈청성분에 미치는 영향. 대한침구학회지. 1987;4(1):117-126.
임종국. 침구치료학. 서울, 집문당, 1983:133,136,169,226.
전국한의과대학. 경혈침구학교실. 침구학(상). 서울, 집문당, 1988:382-384.
통계청. 사망원인통계연보. 통계청, 1997:21-28.
Aho K, Harmsen P, Hatano S, et al. Cerebrovascular disease in the community: Results of a WHO collaborative study. Bull World Health Organ. 1980;58(1):113-130.
Akins PT, Liu PK, Hsu CY. Immediate early gene expression in response to cerebral ischemia. Friend or foe Stroke. 1996;27(9):1682-1687.
Alitalo, R, Partanen J, Pertovaara L, et al. Increased erythroid potentiating activity/tissue inhibitor of metalloproteinases and jun/fos transcription factor complex characterize tumor promoter-induced megakaryoblastic differentiation of K562 leukemia cells. Blood. 1990;75(10):1974-1982.
Caubet JF, Mitjavila MT, Duhart A. et al. Expression of the c-fos protooncogene by human and murine erythroblasts. Blood. 1989;74(3):947-951.
Chen GS, Erdmann W. Effects of acupuncture on tissue oxygenation of the rat brain. South Med J. 1978;71(4):392-395, 398.
Cho ZH, Chung SC, Jones JP, et al. New findings of the correlation between and corresponding brain cortices using functional MRI. Proc Natl Acad Sci USA. 1998;95(5):2670-2673.
Comelli MC, Guidolin D, Seren MS, et al. Time course, localization and pharmacological modulation of immediate early inducible genes, brain-derived neurotrophic factor and trkB messenger RNAs in the rat brain following photochemical stroke. Neuroscience. 1993;55(2):473-490.
Domanska-Janik K, Bong P, Bronisz-Kowalczyk A, et al. API transcriptional factor activation and its relation to apoptosis of hippocampal CA1 pyramidal neurons after transient ischemia in gerbils. J Neurosci Res. 1999;57(6):840-846.
Dony C, Gruss P. Proto-oncogene c-fos expression in growth regions of fetal bone and mesodermal web tissue. Nature. 1987;328(6132):711-714.
Dragunow M, Robertson HA. Brain injury induces c-fos protein(s) in nerve and glial-like cells in adult mammalian brain. Brain Res. 1988;455(2):295-299.
Du Bois-Reymond. Untersuchungen uber Thierische Elektricitat Berlin, Reimer Verlag, 1848.
Duchenne de B. De L'Electrisation Localisee, et de son Application a La Physiologie a La Pathologie et a La Therapeutique. Paris, Bailliere, 1855.
Gao H, Guo J, Zhao P, et al. The neuroprotective effects of electroacupuncture on focal cerebral ischemia in monkey. Acupunct Electro ther Res. 2002;27(1):45-57.
Hinkle L, McCaig CD, Robinson KR. The direction of growth of differentiating neurones and myoblasts from frog embryos in an applied electric field. J Physiol. 1981;314:121-135.
Horn M, Schlote W. Delayed neuronal death and delayed neuronal recovery in the human brain following global ischemia. Acta Neuropathol. 1992;85(1):79-87.
Inoue I, Chen L, Zhou L, et al. Reproduction of scalp acupuncture therapy on strokes in the model rats, spontaneous hypertensive rats-stroke prone (SHR-SP). Neurosci Lett. 2002:333(3):191-194.
Ito U, Spatz M, Walker JT Jr, et al. Experimental cerebral ischemia in mongolian gerbils I. Light microscopic observations. Acta Neuropathol. 1975;32(3):209-223.
Jang MH, Shin MC, Koo GS, et al. Acupuncture decreases nitric oxide synthase expression in periaqueductal gray area of rats with streptozotocin-induced diabetes. Neurosci Lett. 2003;337(3):155-158.
Kim EH, Kim YJ, Lee HJ, et al. Acupuncture increases cell proliferation in dentate gyrus after transient global ischemia in gerbils. Neurosci Lett. 2001;297(1):21-24.
Kouzaride T, Ziff E. The role of the leucine zipper in the fos-jun interaction. Nature. 1988;336(6200):646-651.
Magnusson M, Johansson K, Johansson BB. Sensory stimulation promotes normalization of postural control after stroke. Stroke. 1994:25(6):1176-1180.
Nakabeppu Y, Ryder K, Nathans D. DNA binding activities of three murine Jun proteins: Stimulation by Fos. Cell. 1988:55(5):907-915.
Newberg AB, Alavi A. Changes in the central nervous system during long-duration space flight: Implications for neuro-imaging. Adv Space Res. 1998;22(2):185-196.
Patel N, Poo MM. Orientation of neurite growth by extracellular electric fields. J Neurosci. 1982;2(4):483-496.
Seibert K, Masferrer J, Zhang Y, et al. Mediation of inflammation by cyclooxygenase-2. Agents Actions Suppl. 1995;46:41-50.
Shaulian E, Karin M. AP-1 in cell proliferation and survival. Oncogene. 2001;20(19):2390-2400.
Sheng M, Greenberg ME. The regulation and function of c-fos and other immediate early genes in the nervous system. Neuron. 1990;4(4):477-485.
Shigeno T, Mima T, Takakura K, et al. Amelioration of delayed neuronal death in the hippocampus by nerve growth factor. J Neurosci. 1991;11(9):2914-2919.
Smeyne RJ, Curran T, Morgan JI. Temporal and spatial expression of a fos-lac Z transgene in the developing nervous system. Brain Res Mol Brain Res. 1992:16(1-2):158-162.
Smth WL, DeWitt DL. Biochemistry of prostaglandin endoperoxide H synthase-1 and synthase-2 and their differential susceptibility to nonsteroidal anti-inflammatory drugs. Semin Nephrol. 1995;15(3):179-194.
Uchida S, Kagitani F, Suzuki A, et al. Effect of acupuncture like stimulation on cortical cerebral blood flow in anesthetized rats. Jpn J Physiol. 2000;50(5):495-507.
Wu MT, Hsieh JC, Xiong J, et al. Central nervous pathway for acupunture stimulation: Localization of processign with functional MR imaging of the brain-preliminary experience. Radiology. 1999;212(1):133-141.
Yuan X, Hao X, Lai Z, et al. Effects of acupuncture at fengchi point (GB 20) on cerebral blood flow. J Tradit Chin Med 1998;18(2):102-105.