[논문]진도개에서 발생한 Os clitoris와 Ovotestes를 특징으로 하는 양성자 사례 연구

조성진; 이현아; 박상호; 김옥진

진도개에서 발생한 Os clitoris와 Ovotestes를 특징으로 하는 양성자 사례 연구
An Intersex with Os Clitoris and Ovotestes in Korean Native Jindo Dog 원문보기

Journal of veterinary clinics = 한국임상수의학회지, v.27 no.1 = no.72, 2010년, pp.83 - 87

조성진 (가족동물병원) , 이현아 (원광대학교 동물자원개발연구센터) , 박상호 ((주)DNA Valley) , 김옥진 (원광대학교 동물자원개발연구센터)

초록
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양성자(Hermaphroditism)는 사람과 동물에서 매우 드물게 발생단계의 이상으로 발생한다. 한국에서 개의 양성자 발생 보고는 6 사례가 있으며 그들 모두가 American Cocker Spaniel 품종이었다. 본 연구자들은 진돗개에서 발생한 양성자 사례를 발견하고 그 특징 분석과 병리조직학적 연구 및 분자생물학적 성별감별 연구를 수행하였다. 본 사례연구를 통하여 os clitoris와 ovotestes를 가지며 암컷 성을 가진 양성자 진돗개를 최초로 확인하고 보고하는 바이다.

Abstract ▼ AI-Helper

Hermaphroditism occurs rarely in human and animal populations. Previously, in Korea, The hermaphrodite dog in American Cocker Spaniel dogs have been reported in six cases. However, in this case, we found a hermaphrodite dog in Korean native Jindo dog. The characteristics were its enlarged os clitoris that was shown a reddish fingerlike structure protruding from the vulva. The dog was diagnosed as hermaphroditism with ovotestes. To our knowledge, this report is the first case of hermaphroditism in Korean native dog.

주제어

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가설 설정

Polymerase chain reaction amplification of sex determining region in Y chromosome and agarose gel electrophoresis were used to determine unknown sex. In this study, we used a method based on Y chromosome specific region which is unique to male. As the results, the case WK28052 could be identified as a female sex.

제안 방법

Furthermore, we also met other three reported cases in Korea (1). In this study, we found newly a hermaphrodite dog in Korea native Jindo dog and analyzed the characteristics of the submitted case.
PCR amplification of sex determining region in Y chromosome and agarose gel electrophoresis were used to determine unknown sex of WK28052 sample. In this study, we used a method based on Y chromosome specific region which was unique to male, so its presence or absence in a sample of genomic DNA was indicative of sex. In male, an amplified Yspecific region made a band in agarose gel whereas there was no band in female.
The studied animal (WK28052) was 5-months old Korean Jindo dog with 13 kg body weight and white coat color (Fig 1). It was evaluated with vaginal bleeding, and intersex anomalies. Intersex was identified in the dog with an os clitoris that was buried in the intact vulva.
We analyzed the characteristics of the submitted hermaphrodite case. The morphological observations and surgical dissections were performed. The gonadotectomy, clinical exam and pathological studies were performed.

대상 데이터

In this study, the case (WK28052) was a 5-months old Korea native Jindo dog with external female appearance and the intact vulva was normal structure (Fig 2). However, the dog had the os clitoris buried in the vulva.
SRY primers used in this study were designed according to conserved region of the human pY53.3 DNA sequence (17,19): the upstream primer was 5'-AAGCGACCCATGAATGCATTCATGGTGTGGT-3' and the downstream primer was 5'-GAGGTCGATACTTATAGTTCGGGTATTTCTCTCTGTG-3' (6,19).
The gonads revealed inactive seminiferous tubules and epididymides (1). The gonads were confirmed as testes (1). Previously, we analyzed that the reported hermaphrodite dogs in Korea were American Cocker Spaniel and American Cocker Spaniel dogs have susceptible to hermaphroditism (15).
The studied animal (WK28052) was 5-months old Korean Jindo dog with 13 kg body weight and white coat color (Fig 1). It was evaluated with vaginal bleeding, and intersex anomalies.
Fig 1. The studied animal (WK28052) was 5-months old Korean Jindo dog with 13 kg body weight and white coat color.

성능/효과

In this study, we used a method based on Y chromosome specific region which is unique to male. As the results, the case WK28052 could be identified as a female sex. The dog was diagnosed as hermaphroditism with ovotestes.

참고문헌 (20)

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