목적: 소프트콘택트렌즈 관리용액에 의한 balafilcon A 재질의 실리콘 하이드로겔렌즈에 부착된 지방 침전물 제거효과를 비교하였다. 방법: Balafilcon A 재질의 실리콘 하이드로겔렌즈를 실험실 조건에서 oleic acid, oleic acid methyl ester, cholesterol이 포함된 식염수에 담가 24시간 동안 오염시켰다. 오염된 콘택트렌즈는 식염수로 헹구어준 후 계면활성세척액, 알콜 성분이 포함된 세척액 및 다목적용액으로 각각 세척하였고, 반복적인 오염과 세척효과를 관찰하기 위해서는 오염과 세척과정을 14회 반복하였다. 초음파의 지방침전물 세척 효과를 관찰하기 위하여 오염된 렌즈에 초음파처리를 한 후 처리하지 않은 렌즈와 비교하였다. 지방침전물은 methanol:chloroform (1:1, v/v) 용액으로 추출하고 고성능액체크로마토그래피로 분석하여 정량하였다. 결과: 실리콘 하이드로겔렌즈의 지방세척효과는 알콜성분을 함유한 세척액이 계면활성세척액 및 다목적용액보다 높았으며, 초음파를 함께 처리해준 경우에는 세척 효과가 상승하였다. 콘택트렌즈 관리용액으로 세척한 후에도 지방침전물은 완전히 제거되지 않아 오염과 세척을 반복한 경우 지방 침전물의 양은 지속적으로 증가하였다. 결론: 실리콘 하이드로겔렌즈에 부착되는 지방 침전물에 대한 소프트 콘택트렌즈용 관리용액의 세척효과는 만족할 수준에 미치지 못하기 때문에 실리콘 하이드로겔렌즈의 지방 침전물 제거에 적합한 세척액의 개발이 필요할 것으로 사료된다.
목적: 소프트콘택트렌즈 관리용액에 의한 balafilcon A 재질의 실리콘 하이드로겔렌즈에 부착된 지방 침전물 제거효과를 비교하였다. 방법: Balafilcon A 재질의 실리콘 하이드로겔렌즈를 실험실 조건에서 oleic acid, oleic acid methyl ester, cholesterol이 포함된 식염수에 담가 24시간 동안 오염시켰다. 오염된 콘택트렌즈는 식염수로 헹구어준 후 계면활성세척액, 알콜 성분이 포함된 세척액 및 다목적용액으로 각각 세척하였고, 반복적인 오염과 세척효과를 관찰하기 위해서는 오염과 세척과정을 14회 반복하였다. 초음파의 지방침전물 세척 효과를 관찰하기 위하여 오염된 렌즈에 초음파처리를 한 후 처리하지 않은 렌즈와 비교하였다. 지방침전물은 methanol:chloroform (1:1, v/v) 용액으로 추출하고 고성능액체크로마토그래피로 분석하여 정량하였다. 결과: 실리콘 하이드로겔렌즈의 지방세척효과는 알콜성분을 함유한 세척액이 계면활성세척액 및 다목적용액보다 높았으며, 초음파를 함께 처리해준 경우에는 세척 효과가 상승하였다. 콘택트렌즈 관리용액으로 세척한 후에도 지방침전물은 완전히 제거되지 않아 오염과 세척을 반복한 경우 지방 침전물의 양은 지속적으로 증가하였다. 결론: 실리콘 하이드로겔렌즈에 부착되는 지방 침전물에 대한 소프트 콘택트렌즈용 관리용액의 세척효과는 만족할 수준에 미치지 못하기 때문에 실리콘 하이드로겔렌즈의 지방 침전물 제거에 적합한 세척액의 개발이 필요할 것으로 사료된다.
Purpose: An in vitro study was conducted to compare the lipid cleaning efficacy of care solutions on balafilcon A silicone hydrogel (SiHy) lens. Methods: Lipid spoilation was performed by incubating balafilcon A SiHy lenses in phosphate buffered saline (PBS) containing oleic acid, oleic acid methyl ...
Purpose: An in vitro study was conducted to compare the lipid cleaning efficacy of care solutions on balafilcon A silicone hydrogel (SiHy) lens. Methods: Lipid spoilation was performed by incubating balafilcon A SiHy lenses in phosphate buffered saline (PBS) containing oleic acid, oleic acid methyl ester and cholesterol. Spoiled contact lenses rinsed with PBS were cleaned with surfactant cleaner, alcohol containing cleaner and multipurpose solution (MPS) respectively and repetitive spoilation and cleaning was conducted up to 14 times. To observe the cleaning effect of ultrasonic wave on the lipid deposit, each spoiled lens was ultrasonicated and then compared with non-sonicated lens. Lipids deposit on the contact lenses was extracted by methanol:chloroform (1:1, v/v) solution. High performance liquid chromatography was used to analyze and quantify lipid deposit extracts. Results: The effectiveness of alcohol containing surfactant cleaner on the lipid deposits was better than that of surfactant cleaner and MPS, and the cleaning efficacy was significantly higher in the ultrasonic wave treated group. Lipid deposits were not removed completely by contact lens care solutions so that lipid deposits increased continuously and cumulatively. Conclusions: The cleaning efficacy of contact lens care solutions was not satisfactory to remove lipid deposits on the SiHy lens that new cleaning products specially designed for SiHy lenses are needed to develop.
Purpose: An in vitro study was conducted to compare the lipid cleaning efficacy of care solutions on balafilcon A silicone hydrogel (SiHy) lens. Methods: Lipid spoilation was performed by incubating balafilcon A SiHy lenses in phosphate buffered saline (PBS) containing oleic acid, oleic acid methyl ester and cholesterol. Spoiled contact lenses rinsed with PBS were cleaned with surfactant cleaner, alcohol containing cleaner and multipurpose solution (MPS) respectively and repetitive spoilation and cleaning was conducted up to 14 times. To observe the cleaning effect of ultrasonic wave on the lipid deposit, each spoiled lens was ultrasonicated and then compared with non-sonicated lens. Lipids deposit on the contact lenses was extracted by methanol:chloroform (1:1, v/v) solution. High performance liquid chromatography was used to analyze and quantify lipid deposit extracts. Results: The effectiveness of alcohol containing surfactant cleaner on the lipid deposits was better than that of surfactant cleaner and MPS, and the cleaning efficacy was significantly higher in the ultrasonic wave treated group. Lipid deposits were not removed completely by contact lens care solutions so that lipid deposits increased continuously and cumulatively. Conclusions: The cleaning efficacy of contact lens care solutions was not satisfactory to remove lipid deposits on the SiHy lens that new cleaning products specially designed for SiHy lenses are needed to develop.
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제안 방법
Lens parameters were measured with Base Curve Screen (for base curve and total diameter, JCF, OPTIMEC), Electronic Thickness Gauge (for center thickness, ET-3, Rehder Development Company), and Magnon (for lens power, LM-770, NIDEC) before and after ultrasonic wave treatment respectively.
1. Representative high-performance liquid chromatogram using the HPLC method from a 1 mg/mL standard sample mixture of oleic acid, oleic acid methyl ester, and cholesterol having retention times of 4.7, 9.0, and 26.1 minutes, respectively.
To observe the effect of ultrasonic wave on the lipid deposited contact lens, each lens was placed in saline containing 3 drops of care solutions in the lens case, and was then ultrasonicated for 1 minute, 5 times in a water bath (Branson 5210 ultrasonic cleaner, 40 KHz) and then digitally cleaned.
대상 데이터
Table 1 details some of the characteristics of these lens. Five lenses of each group were used for the analysis.
Lens material used in this study was balafilcon A (PureVision, Bausch & Lomb). Table 1 details some of the characteristics of these lens.
참고문헌 (23)
Jones L., Mann A., Evans K., Franklin V., and Tighe B., "An in vivo comparison of the kinetics of protein and lipid deposition on group II and group IV frequentreplacement contact lenses", Optom. Vis. Sci., 77(10):503-510(2000).
Carney F. P., Nash W. L., and Sentell K. B., ''The adsorption of major tear film lipids in vitro to various silicone hydrogels over time", Invest. Ophthalmol. Vis. Sci., 49(1):120-124 (2008).
Garrett Q., Laycock B., and Garrett R. W., "Hydrogel lens monomer constituents modulate protein sorption", Invest. Ophthalmol. Vis. Sci., 41(7):1687-1695(2000).
Gellatly K. W., Brennan N. A., and Efron N., "Visual decrement with deposit accumulation on HEMA contact lenses", Am. J. Optom. Physiol. Opt., 65(12):937-941(1988).
Fonn D., ''Targeting contact lens induced dryness and discomfort: what properties will make lenses more comfortable", Optom. Vis. Sci., 84(4):279-285(2007).
McCulley J. P. and Shine W., "A compositional based model for the tear film lipid layer", Trans. Am. Ophthalmol. Soc., 95(1):79-88(1997).
Greiner J. V., Glonek T., Korb D. R., Booth R., and Leathy C. D., "Phospholipids in meibomian gland secretion", Ophthalmic Res., 28(1):44-49(1996).
Sweeney D., "Silicone hydrogel materials", 1st Ed. Butterworth-Heinemann, London, UK, pp. 1-21(2000).
Sweeney D., "Silicone hydrogel materials", 2nd Ed. Butterworth- Heinemann, London, UK, pp. 1-27(2004).
Lopez-Alemany A., Compan V., and Refojo M. F., "Porous structure of Purevision versus Focus Night & Day and conventional hydrogel contact lenses", J. Biomed. Mate. Res., 63(3):319-325(2002)
Jones L., Senchyna M., and Glasier M., "Lysozyme and lipid deposition on silicone hydrogel contact lens materials", Eye Contact Lens, 29(1S):S75-S79(2003).
You-sun K. and Koon-ja L., "Evaluation of lipids adsorbed on silicone hydrogel contact lenses using high performance liquid chromatography analytical method", Korean J. Vis. Sci., 12(2):127-138(2010).
Bontempo A. R. and Rapp J., "Protein and lipid deposition onto hydrophilic contact lenses in vivo", CLAO J., 27(1):75-80(2001).
Subbaraman L. N., Glasier M. A., Senchyna M., Sheardown H., and Jones L., "Kinetics of in vitro Iysozume deosition on silicone hydrogel, PMMA, and FDA groups I, II and IV contact lens materials", Curr. Eye Res., 31(10):787-796(2006).
Maissa C., Guillon M., Girared-Claudon K., and Cooper P., "Tear lipid composition of hydrogel contact lens wearers", Journal of Cornea and External Disease, 19(6): p.S103(2000).
Maziarz P. E., Stachowski M. J., and Liu M. S., "Lipid deposition on silicone hydrogel lenses, Part I: Quantification of oleic acid, oleic acid methyl ester, and cholesterol", Eye Contact Lens, 32(6):300-307(2006).
Efron N., Lowe R., Vallas V., and Grusiner E., "Clinical efficacy of standing wave and ultrasound for cleaning and disinfecting contact lenses", Int. Contact Lens Clinic, 18(1-2):24-29(1991).
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