본 실험은 7가지의 칼슘제제; calcium sulfate dihydrate, calcium chloride, calcium nitrate, calcium oxide, calcium hydroxide, calcium carbonate, calcium hydride가 포함된 PDA배지에서 잿빛곰팡이 균의 방제효과를 알고자 실시되었다. 선발된 칼슘제제의 pH는 8.2-10으로 대조구인 pH 6.6보다는 높게 측정되었다. 7가지 칼슘제제가 포함된 PDA배지에서의 잿빛곰팡이병 방제 colony size(mm) 결과는 calcium carbonate, calcium oxide, calcium hydride, calcium hydroxide가 다른 칼슘제제들 보다 잿빛곰팡이병 억제에 효과가 좋았다. 선택된 4개의 칼슘제제를 식물체에 처리 한 후 잿빛곰팡이병의 포자를 살포한 실험과 희석된 4개의 칼슘제제)를 표준 양액재배에 첨부하여 한 달간 양액을 공급한 후 잿빛곰팡이병의 포자를 접종한 실험결과는 칼슘제제를 처리하지 않은 대조구보다는 칼슘제제를 처리한 실험구에서 잿빛곰팡이병의 발생률이 대체적으로 낮았다. 칼슘제제를 처리한 파프리카 식물체에서 잿빛곰팡이병 억제에 대하여서는 칼슘제제 처리에 따른 어느 정도의 염기스트레스의 영향 등으로 볼 수가 있겠다.
본 실험은 7가지의 칼슘제제; calcium sulfate dihydrate, calcium chloride, calcium nitrate, calcium oxide, calcium hydroxide, calcium carbonate, calcium hydride가 포함된 PDA배지에서 잿빛곰팡이 균의 방제효과를 알고자 실시되었다. 선발된 칼슘제제의 pH는 8.2-10으로 대조구인 pH 6.6보다는 높게 측정되었다. 7가지 칼슘제제가 포함된 PDA배지에서의 잿빛곰팡이병 방제 colony size(mm) 결과는 calcium carbonate, calcium oxide, calcium hydride, calcium hydroxide가 다른 칼슘제제들 보다 잿빛곰팡이병 억제에 효과가 좋았다. 선택된 4개의 칼슘제제를 식물체에 처리 한 후 잿빛곰팡이병의 포자를 살포한 실험과 희석된 4개의 칼슘제제)를 표준 양액재배에 첨부하여 한 달간 양액을 공급한 후 잿빛곰팡이병의 포자를 접종한 실험결과는 칼슘제제를 처리하지 않은 대조구보다는 칼슘제제를 처리한 실험구에서 잿빛곰팡이병의 발생률이 대체적으로 낮았다. 칼슘제제를 처리한 파프리카 식물체에서 잿빛곰팡이병 억제에 대하여서는 칼슘제제 처리에 따른 어느 정도의 염기스트레스의 영향 등으로 볼 수가 있겠다.
Plant diseases including gray mold caused by Botrytis cinerea are often reduced when calcium compounds are used as alternative materials in paprika. However, much less information is available about the effects of calcium compounds on controlling of $B.$$cinerea$. Seven calciu...
Plant diseases including gray mold caused by Botrytis cinerea are often reduced when calcium compounds are used as alternative materials in paprika. However, much less information is available about the effects of calcium compounds on controlling of $B.$$cinerea$. Seven calcium compounds such as calcium sulfate dihydrate, calcium chloride, calcium nitrate, calcium oxide, calcium hydroxide, calcium carbonate, and calcium hydride were evaluated for their effectiveness against $B.$$cinerea$ on potato dextrose agar medium. The pH of selected calcium compounds was higher (pH 8.2-10) than that of the control (pH 6.6). Calcium carbonate, calcium oxide, calcium hydride, and calcium hydroxide among seven calcium compounds were more effectively inhibited the growth of $B.$$cinerea$ than other calcium compounds. In the case of spraying the spore suspension on paprika applied with the selected four calcium compounds and supplied with the selected calcium supplements in a hydroponic culture system, the paprika treated with calcium compounds showed less severity of disease than those untreated plants. On the basis of our results, we propose that the suppressive effects of calcium compounds on $B.$$cinerea$ in paprika resulted from the supply of calcium and a certain degree of salt stress.
Plant diseases including gray mold caused by Botrytis cinerea are often reduced when calcium compounds are used as alternative materials in paprika. However, much less information is available about the effects of calcium compounds on controlling of $B.$$cinerea$. Seven calcium compounds such as calcium sulfate dihydrate, calcium chloride, calcium nitrate, calcium oxide, calcium hydroxide, calcium carbonate, and calcium hydride were evaluated for their effectiveness against $B.$$cinerea$ on potato dextrose agar medium. The pH of selected calcium compounds was higher (pH 8.2-10) than that of the control (pH 6.6). Calcium carbonate, calcium oxide, calcium hydride, and calcium hydroxide among seven calcium compounds were more effectively inhibited the growth of $B.$$cinerea$ than other calcium compounds. In the case of spraying the spore suspension on paprika applied with the selected four calcium compounds and supplied with the selected calcium supplements in a hydroponic culture system, the paprika treated with calcium compounds showed less severity of disease than those untreated plants. On the basis of our results, we propose that the suppressive effects of calcium compounds on $B.$$cinerea$ in paprika resulted from the supply of calcium and a certain degree of salt stress.
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제안 방법
Only the fully expanded leaf and stem from each plant was inoculated with 50 mL of the suspension using a low-pressure sprayer. All of the treatments were applied as post-inoculation foliar sprays into plants on the 25th of October and arranged in a randomized block design with 10 replicate plants per treatment. Three replicates were performed per treatment.
For the purpose of this investigation, we assessed the mycelial growth of B. cinerea occurring in paprika plants, depending on different calcium compounds on PDA media, together with the role of calcium for controlling B. cinerea. Our data showed that the colony sizes of B.
The first objective was to study the ability of calcium compounds to enhance the control activity against B. cinerea on potato dextrose agar (PDA) media containing calcium compounds as well as the infection of paprika plants by B. cinerea. The second objective was to examine the responses of the amending nutrient solutions with calcium supplements against B.
cinerea. The second objective was to examine the responses of the amending nutrient solutions with calcium supplements against B. cinerea during the periods of occurrence on paprika grown in nutrient solutions.
, Korea) on 16 March, 2008. Thereafter, seedlings were managed in the same manners as described previously and the standard nutrient solution was amended with the selected four calcium compounds, which were diluted 10 times more than the containing of calcium compounds on PDA media, separately and supplied each nutrient system line for one month, especially in the period of occurrence of B. cinerea between September and October in 2008, as an alternative method to control of B. cinerea. To measure the pH of each amending nutrient solution, we used the portable pH meter (Argus, Sentron, Netherlands) and tried to maintain between 5.
대상 데이터
All of the treatments were applied as post-inoculation foliar sprays into plants on the 25th of October and arranged in a randomized block design with 10 replicate plants per treatment. Three replicates were performed per treatment. The existing disease for each infected site per plant was measured one month later, on the 25th of November in 2008.
데이터처리
All of the data was analyzed by analysis of variance, and mean separation done with Duncan’s multiple range test (DMRT) at P = 0.05 using Statistical Analysis System (SAS, 2003, Institute Inc., Cary, NC, USA) and figure program (Sigma Plot 2001, Phil Science Co., Korea).
성능/효과
The disease occurrence of the plants grown in calcium hydroxide showed 20% and 40% in ‘Cupra’ and ‘Boogie’; also, the disease occurrence of the plants grown in calcium carbonate were presented as 20% and 0% in ‘Cupra’ and ‘Boogie’, respectively.
The greatest decrease in disease occurrence was recorded in the calcium oxide treatment, which showed 0% disease incidence in ‘Cupra’ and ‘Boogie’, respectively, compared with control (standard nutrient solution) as 66.7% and 70.0% in ‘Cupra’ and ‘Boogie’, respectively.
1 and comparison of seedling test in Table 2. The results showed that the increased pH of calcium compounds was more effective to inhibit the growth of B. cinerea than the lowered pH of calcium compounds.
후속연구
It is necessary to determine effective levels of calcium for disease suppression in the field or in the nutrient solutions. Moreover, further research is needed on the central role of the mechanisms involved in disease reduction to the application of calcium.
참고문헌 (12)
Chardonnet, C.O., C.E. Sams, and W.S. Conway. 1999. Calcium effect on the mycelial cell walls of Botrytis cinerea. Phytochem. 52:967-973.
Charlotte, C. and B. Doneche. 2002. Purification and characterization of two isozymes of polygalacturonase from Botrytis cinerea. Effect of calcium ions on polygalacturonase activity. Microbiol. Res. 157:183-189.
Elad, Y., H. Yunis, and H. Volpin. 1993. Effect of nutrition on susceptibility of cucumber, eggplant, and pepper crops to Botrytis cinerea. Can. J. Botany 71:602-608.
Guillem, S., C. Eva, B. Celia, A. Mannuel, and T. Isabel. 2007. The suppressive effects of composts used as growth media against Botrytis cinerea in cucumber plants. J. Eur. Plant Pathol. 117:393-402.
McLaughlin, R.J., M.E. Wisniewski, C.L. Wilson, and E. Chalutz. 1990. Effect of inoculum concentration and salt solutions on biological control of postharvest diseases of apple with Candida sp. J. Phytopathol. 80:456-461.
Mikani, A., H.R. Etebarian, P.L. Sholberg, D.T.O. Gorman, S. Stokes, and A. Alizadeh. 2008. Biological control of apple gray mold caused by Botrytis mali with Pseudomonas fluorescens strains. Posthar. Biol. and Technol. 48:107-112.
Raposo, R., J. Delcan, V. Gomez, and P. Melgarejo. 1996. Distribution and fitness of isolates of Botrytis cinerea with multiple fungicides resistance in Spanish greenhouses. Plant Pathol. 45:497-505.
Stanghellini, M.E., S.L. Rasmussen, D.H. Kim, and P.A. Rorabaugh. 1996. Efficacy of nonionic surfactant in the control of zoospore spread of Pythium aphanidermatum in a recirculating hydroponic system. Plant Dis. 80:422-428.
Sugimoto, T., M. Anio, M. Sugimoto, and K. Watanabe. 2005. Reduction of Phytophthora stem rot disease soybeans by the application of $CaCl_{2}$ and $Ca(NO_{3})_{2}$ . J. Phytopathol. 153:536-543.
Yoon, C.S., E.H. Ju, Y.R. Yeoung, and B.S. Kim. 2008. Survey of fungicide resistance for chemical control of Botrytis cinerea on Paprika. J. Plant Pathol. 24:447-452.
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