$\require{mediawiki-texvc}$

연합인증

연합인증 가입 기관의 연구자들은 소속기관의 인증정보(ID와 암호)를 이용해 다른 대학, 연구기관, 서비스 공급자의 다양한 온라인 자원과 연구 데이터를 이용할 수 있습니다.

이는 여행자가 자국에서 발행 받은 여권으로 세계 각국을 자유롭게 여행할 수 있는 것과 같습니다.

연합인증으로 이용이 가능한 서비스는 NTIS, DataON, Edison, Kafe, Webinar 등이 있습니다.

한번의 인증절차만으로 연합인증 가입 서비스에 추가 로그인 없이 이용이 가능합니다.

다만, 연합인증을 위해서는 최초 1회만 인증 절차가 필요합니다. (회원이 아닐 경우 회원 가입이 필요합니다.)

연합인증 절차는 다음과 같습니다.

최초이용시에는
ScienceON에 로그인 → 연합인증 서비스 접속 → 로그인 (본인 확인 또는 회원가입) → 서비스 이용

그 이후에는
ScienceON 로그인 → 연합인증 서비스 접속 → 서비스 이용

연합인증을 활용하시면 KISTI가 제공하는 다양한 서비스를 편리하게 이용하실 수 있습니다.

Differentiation of Three Lactobacillus rhamnosus Strains (E/N, Oxy, and Pen) by SDS-PAGE and Two-Dimensional Electrophoresis of Surface-Associated Proteins 원문보기

Journal of microbiology and biotechnology, v.20 no.3, 2010년, pp.558 - 562  

Jarocki, P. (Department of Biotechnology, Human Nutrition and Food Commodities, University of Life Science in Lublin) ,  Podlesny, M. (Department of Biotechnology, Human Nutrition and Food Commodities, University of Life Science in Lublin) ,  Wasko, A. (Department of Biotechnology, Human Nutrition and Food Commodities, University of Life Science in Lublin) ,  Siuda, A. (Department of Biotechnology, Human Nutrition and Food Commodities, University of Life Science in Lublin) ,  Targonski, Z. (Department of Biotechnology, Human Nutrition and Food Commodities, University of Life Science in Lublin)

Abstract ▼ AI-Helper 아이콘AI-Helper

SDS-PAGE of extracted surface-associated proteins of Lactobacillus rhamnosus strains E/N, Oxy, and Pen, was performed. The obtained protein patterns allowed differentiation of the examined strains, which was not accomplished by the commonly used RAPD genotypic method. The differentiation by the SDS-...

주제어

#Lactobacillus   #polyacrylamide gel electrophoresis   #random amplification of polymorphic DNA   #surface-associated proteins  

AI 본문요약
AI-Helper 아이콘 AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

문제 정의

  • The objective of this study was to compare ttie discriminatory power of two rapid techniques (RAPD and SDS-PAGE) of surface-associated proteins for the identification of closely related lactobacilli species and strains. Additionally, we used SDS-PAGE and two-dimensional (2D) electrophoresis analysis of surface-associated proteins for the differentiation of L.
본문요약 정보가 도움이 되었나요?

참고문헌 (24)

  1. Alander, M., R. Satokari, R. Korpela, M. Saxellin, T. Vilpponen-Salmela, T. Mattila-Sandholm, and A. von Wright. 1999. Persistence of colonization of human colonic mucosa by a probiotic strain, Lactobacillus rhamnosus GG, after oral consumption. Appl. Environ. Microbiol. 65: 351-354. 

    상세보기

  2. Axelsson, L. 2004. Lactic acid bacteria: Classification and physiology, pp. 1-66. In S. Salminen, A. von Wright, and A. Ouwehand (eds.). Lactic Acid Bacteria. Microbiological and Functional Aspects, 3rd Ed. Marcel Dekker, New York. 

  3. Benito, M. J., M. J. Serradilla, S. Ruiz-Moyano, A. Martin, F. Perez-Nevado, and M. G. Cordoba. 2008. Rapid differentiation of lactic acid bacteria from autochthonous fermentation of Iberian dry-fermented sausages. Meat Sci. 80: 656-661. 

    상세보기 crossref

  4. Gatti, M., M. E. Fornasari, and E. Neviani. 2001. Differentiation of Lactobacillus delbrueckii subsp. bulgaricus and Lactobacillus delbrueckii subsp. lactis by SDS-PAGE of cell-wall proteins. Lett. Appl. Microbiol. 32: 352-356. 

    상세보기 crossref

  5. Goderska, K., M. Czarnecka, and Z. Czarnecki. 2002. Survival rate of chosen Lactobacillus bacteria type in media of different pH. EJPAU Food Sci. Technol. 5: 1-9. 

  6. Gu, R., Z. Yang, J. Cai, Z. Li, S. Chen, and Z. Luo. 2008. Analysis of different Lactobacillus rhamnosus by random amplified polymorphic DNA. Acta Microbiol. Sin. 48: 426-431. 

  7. Hebert, E. M., R. R. Raya, and G. S. de Giori. 2000. Use of SDS-PAGE of cell-wall proteins for rapid differentiation of Lactobacillus delbrueckii subsp. lactis and Lactobacillus helveticus. Biotechnol. Lett. 22: 1003-1006. 

    상세보기 crossref

  8. Kwon, O. S. 2000. Characterization of isolated Lactobacillus spp. and classification by RAPD-PCR analysis. J. Microbiol. 38: 137-144. 

    원문보기 상세보기

  9. Ma, D., P. Forsythe, and J. Bienenstock. 2004. Live Lactobacillus reuteri is essential for the inhibitory effect on tumor necrosis factor alpha-induced interleukin-8 expression. Infect. Immun. 72: 5308-5314. 

    상세보기 crossref

  10. Nguyen, T. D. T., J. H. Kang, and M. S. Lee. 2007. Characterization of Lactobacillus plantarum PH04 a potential probiotic bacterium with cholesterol-lowering effects. Int. J. Food. Microbiol. 113: 358-361. 

    상세보기 crossref

  11. Nigatu, A., S. Ahrne, and G. Molin. 2001. Randomly amplified polymorphic DNA (RAPD) profiles for the distinction of Lactobacillus species. Antonie van Leeuwenhoek 79: 1-6. 

    상세보기 crossref

  12. Ouwehand, A. C., S. Salminen, and E. Isolauri. 2002. Probiotics: An overview of beneficial effects. Antonie van Leeuwenhoek 82: 279-289. 

    상세보기 crossref

  13. Pot, B., P. Vandamme, and K. Kersters. 1994. Analysis of electrophoretic whole-organism protein fingerprints, pp. 493-521. In M. Goodfellow and A. G. O'Donnel (eds.). Chemical Methods in Prokaryotic Systematics. Wiley, Chichester, U.K. 

  14. Rodtong, S. and G. W. Tannock. 1993. Differentiation of Lactobacillus strains by ribotyping. Appl. Environ. Microbiol. 59: 3480-3484. 

    상세보기

  15. Ruszczy ski, M., A. Radzikowski, and H. Szajewska. 2008. Clinical trial: Effectiveness of Lactobacillus rhamnosus (strains E/N, Oxy and Pen) in the prevention of antibiotic-associated diarrhoea in children. Aliment. Pharm. Therap. 28: 154-161. 

    상세보기 crossref

  16. Sanchez, B., P. Bressollier, S. Chaignepain, J. M. Schmitter, and M. C. Urdaci. 2008. Identification of surface-associated proteins in the probiotic bacterium Lactobacillus rhamnosus GG. Int. Dairy J. 19: 85-88. 

  17. Sanchez, I., S. Sesena, and L. L. Palop. 2004. Polyphasic study of the genetic diversity of lactobacilli associated with "Almagro" eggplants spontaneous fermentation, based on combined numerical analysis of randomly amplified polymorphic DNA and pulsedfield electrophoresis patterns. J. Appl. Microbiol. 97: 446-458. 

    상세보기 crossref

  18. Singh, S., P. Goswami, R. Singh, and K. J. Heller. 2009. Application of molecular identification tools for Lactobacillus, with a focus on discrimination between closely related species: A review. LWT Food Sci. Technol. 42: 448-457. 

    상세보기 crossref

  19. Stiles, N. E. 1996. Biopreservation by lactic acid bacteria. Antonie van Leeuwenhoek 70: 331-345. 

    상세보기 crossref

  20. Tilsala-Timisjarvi, A. and T. Alatossava. 1998. Strain-specific identification of probiotic Lactobacillus rhamnosus with randomly amplified polymorphic DNA-derived PCR primers. Appl. Environ. Microbiol. 64: 4816-4819. 

    상세보기

  21. Tynkkynen, S., R. Satokari, M. Saarela, T. Mattila-Sandholm, and M. Saxelin. 1999. Comparison of ribotyping, randomly amplified polymorphic DNA analysis, and pulsed-field gel electrophoresis in typing of Lactobacillus rhamnosus and L. casei strains. Appl. Environ. Microbiol. 65: 3908-3914. 

    상세보기

  22. Varmanen, P., T. Rantanen, A. Palva, and S. Tynkkynen. 1998. Cloning and characterization of a prolinase gene (pepR) from Lactobacillus rhamnosus. Appl. Environ. Microbiol. 64: 1831-1836. 

    상세보기

  23. Wiatrzyk, A., U. Czajka, and B. Buholc. 2007. Resistance to antibiotics of Lactobacillus and Bifidobacterium strains present in probiotics preparations. Ped. Pol. 82: 910-915. 

    상세보기 crossref

  24. Wright, A., R. Wait, S. Begum, B. Crossett, J. Nagy, K. Brown, and N. Fairweather. 2005. Proteomic analysis of cell surface proteins from Clostridium difficile. Proteomics 5: 2443-2452. 

    상세보기 crossref

관련 콘텐츠

오픈액세스(OA) 유형

GOLD

오픈액세스 학술지에 출판된 논문

이 논문과 함께 이용한 콘텐츠

섹션별 컨텐츠 바로가기

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

AI-Helper 아이콘
AI-Helper
안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

선택된 텍스트

맨위로