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Abstract ▼ AI-Helper

A new selectable marker system has been adapted for use in Agrobacterium mediated transformation of Chinese cabbage. This selection system utilizes the pmi gene encoding for phosphomannose-isomerase that converts mannose-6-phosphate to fructose-6-phosphate. Only transgenic plants were able to metabo...

A new selectable marker system has been adapted for use in Agrobacterium mediated transformation of Chinese cabbage. This selection system utilizes the pmi gene encoding for phosphomannose-isomerase that converts mannose-6-phosphate to fructose-6-phosphate. Only transgenic plants were able to metabolize the selection agent, mannose, into a usable source of carbon, fructose. Cotyledon explants from 5-day-old seedlings with 2 days pre-culture were infected with Agrobacterium tumefaciens strain LBA4404 harboring a new constructed vector pNWBHSP101 containing the heat tolerance responsible heat shock protein 101 (HSP101) gene with full codon modification. After culture and selection on MS medium supplemented with 3 mg/L BAP, 1 mg/L NAA, 0.6% mannose, 2.0% sucrose. Polymerase chain reaction, Southern blot analysis and Northern blot analysis were used to identify and characterize the transgenic plants with the integrated HSP101 gene. Transgenic plants have been established in soil and flowered in the greenhouse.