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Anthocyanins in Cowpea [Vigna unguiculata (L.) Walp. ssp. unguiculata

Food science and biotechnology v.19 no.3 , 2010년, pp.821 - 826  
Ha, Tae-Joung (Department of Functional Crop, National Institute of Crop Science (NICS), Rural Development Administration ) ;  Lee, Myoung-Hee ( Department of Functional Crop, National Institute of Crop Science (NICS), Rural Development Administration ) ;  Jeong, Yu-Na ( Department of Functional Crop, National Institute of Crop Science (NICS), Rural Development Administration ) ;  Lee, Jin-Hwan ( Department of Functional Crop, National Institute of Crop Science (NICS), Rural Development Administration ) ;  Han, Sang-Ik ( Department of Functional Crop, National Institute of Crop Science (NICS), Rural Development Administration ) ;  Park, Chang-Hwan ( Department of Functional Crop, National Institute of Crop Science (NICS), Rural Development Administration ) ;  Pae, Suk-Bok ( Department of Functional Crop, National Institute of Crop Science (NICS), Rural Development Administration ) ;  Hwang, Chung-Dong ( Department of Functional Crop, National Institute of Crop Science (NICS), Rural Development Administration ) ;  Baek, In-Youl ( Department of Functional Crop, National Institute of Crop Science (NICS), Rural Development Administration ) ;  Park, Keum-Yong ( Department of Funct)
Abstract

The aim of this study was to isolate and identify the anthocyanins in the black seed coated cowpea [Vigna unguiculata (L.) Walp. ssp. unguiculata] using reverse phase C-18 open column chromatography and high performance liquid chromatography (HPLC) with diode array detection and electro spray ionization/mass spectrometry (DAD-ESI/MS) analysis, respectively. Anthocyanins were extracted from the coat of black cowpea with 1% trifluoroacetic acid (TFA) in methanol, isolated by RP-C-18 column chromatography, and their structures elucidated by 1D and 2D nuclear magnetic resonance (NMR) spectroscopy. The isolated anthocyanins were characterized as delphinidin-3-O-glucoside (2) and cyanidin-3-Oglucoside (4). Furthermore, 5 minor anthocyanins were detected and identified as delphinidin-3-O-galactoside (1), cyanidin-3-O-galactoside (3), petunidin-3-O-glucoside (5), peonidin-3-O-glucoside (7), and malvidin-3-O-glucoside (8) based on the fragmentation patterns of HPLC-DAD-ESI/MS analysis.

주제어

#Vigna unguiculata   #cowpea   #anthocyanin   #high performance liquid chromatography  

참고문헌 (23)

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이 논문을 인용한 문헌 (3)

  1. Jung, Yang Sook ; Choi, Kyung-Jin ; Kang, Hang-Won ; Bae, Do-Gyu 2011. "Research on the Colorants Extraction from Black Cowpea Seed Coats and their Storage Stability" 韓國資源植物學會誌 = Korean journal of plant resources, 24(5): 499~506 
  2. Kim, Dong-Kwan ; Son, Dong-Mo ; Choi, Jin-Gyung ; Shin, Hae-Ryong ; Choi, Kyeong-Ju ; Lee, Jeongran ; Lee, Kyung-Dong ; Rim, Yo-Sup 2013. "Agronomic Characteristics and Seed Quality of Cowpea (Vigna unguiculata L.) Germplasm" Korean journal of crop science = 韓國作物學會誌, 58(1): 1~7 
  3. 2014. "" Fibers and polymers, 15(1): 138~144 

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