[논문]Evaluation of vitrification for cryopreservation of teeth

Dissanayake, Surangi C.; Che, Zhong-Min; Choi, Seong-Ho; Lee, Seung-Jong; Kim, Jin

doi:10.5051/jpis.2010.40.3.111

Evaluation of vitrification for cryopreservation of teeth 원문보기

Journal of periodontal & implant science, v.40 no.3, 2010년, pp.111 - 118

Dissanayake, Surangi C. (Oral Cancer Research Institute, Department of Oral Pathology, Yonsei University College of Dentistry) , Che, Zhong-Min (Oral Cancer Research Institute, Department of Oral Pathology, Yonsei University College of Dentistry) , Choi, Seong-Ho (Department of Periodontology, Yonsei University College of Dentistry) , Lee, Seung-Jong (Department of Conservative Dentistry, Yonsei University College of Dentistry) , Kim, Jin (Oral Cancer Research Institute, Department of Oral Pathology, Yonsei University College of Dentistry)

Abstract ▼ AI-Helper

Purpose: The aim of this study was to investigate whether vitrification in the cryopreservation of periodontal ligament (PDL) cells could be useful for tooth banking. Methods: In step 1, primary cultured human PDL cells were cryopreserved in 100% conventional cryopreservation media and 100% vitrification media (ESF40 media) in different temperatures for 2 weeks. In step 2, a series of modified vitrification formulae named T1 (75% vitrification media + 25% F-media), T2 (50% vitrification media + 50% F-media) and T3 (25% vitrification media + 75% F-media) were used to store PDL cells for 2 weeks and 4 weeks in liquid nitrogen. MTT assay was performed to examine the viability of PDL cells. Results: Maximum cell viability was achieved in cells stored in 100% conventional cryopreservation media at $-196^{\circ}C$ (positive control group) in step 1. Compared to the positive control group, viability of the cells stored in 100% vitrification media was very low as 10% in all test conditions. In step 2, as the percentage of vitrification media decreased, the cell viability increased in cells stored for 2 weeks. In 4-week storage of cells in step 2, higher cell viability was observed in the T2 group than the other vitrification formulae while the positive control group had the highest viability. There was no statistically significant difference in the cell viability of 2-week and 4-week stored cells in the T2 group. Conclusions: These observations indicate 100% vitrification media is not successful in PDL cell cryopreservation. Conventional cryopreservation media is currently the most appropriate media type for this purpose while T2 media would be interesting to test for long-term storage of PDL cells.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

문제 정의

As vitrification has shown cell functional and survival recovery superior to conventional cryopreservation methods, in this study we examined the effects of vitrification on PDL cells. For successful tooth transplantation, the integrity of PDL cells is an utmost need; therefore, the purpose of this study was to evaluate whether vitrification can be useful for tooth cryopreservation.
For successful tooth transplantation, the integrity of PDL cells is an utmost need; therefore, the purpose of this study was to evaluate whether vitrification can be useful for tooth cryopreservation. In this study we tested and compared the viability of PDL cells after vitrification or conventional cryopreservation, each for two and four weeks.
[3] reported the occurrence of a longitudinal fracture in 25% of conventionally cryopreserved teeth during a hardness test. Therefore, this study was designed to develop a new freezing method for tooth preservation.

제안 방법

The storage cell retrieval method was similar to that of step 1. Cell retrieval was done after 2 weeks and 4 weeks of cryopreservation.
For successful tooth transplantation, the integrity of PDL cells is an utmost need; therefore, the purpose of this study was to evaluate whether vitrification can be useful for tooth cryopreservation. In this study we tested and compared the viability of PDL cells after vitrification or conventional cryopreservation, each for two and four weeks.
The aim was to compare the viability of stock cells stored in these two different cryoprofiles, namely the vitrification method and conventional method at a temperature of -i96℃. Cells stocked in conventional media stored at -ig6℃ were used as the positive control.

대상 데이터

08% ethylenediamine tetraacetic acid (EDTA), and harvested in F-media. Five cryotubes were prepared for each experimental group. One mL of resuspended cells was aliquoted into each cryotube, and immediately placed on ice after proper tightening of the caps.

이론/모형

Louis, USA). Vitrification media was prepared according to the formula developed by Kasai and Mukaida [6] named ESF40. This comprises 40% ethylene glycol (EG) (WAKO Pure Chemical Industries Ltd.

성능/효과

1. According to the results in step 1, the positive control group or the conventional cryopreservation group stored at -196℃ showed maximum cell viability while all the other groups showed much decreased cell survival closer to 10% when compared to the positive control group.

참고문헌 (19)

1 Kristerson L Johansson LA Kisch J Stadler LE Autotransplantation of third molars as treatment in advanced periodontal disease J Clin Periodontol 1991 18 521 528 1894746

상세보기
2 Schwartz O Andreasen JO Greve T Cryopreservation before replantation of mature teeth in monkeys. (II). Effect of preincubation, different freezing and equilibration rates and endodontic treatment upon periodontal healing Int J Oral Surg 1985 14 350 361 3928511
3 Oh YH Che ZM Hong JC Lee EJ Lee SJ Kim J Cryopreservation of human teeth for future organization of a tooth bank: a preliminary study Cryobiology 2005 51 322 329 16297377

상세보기
4 Temmerman L De Pauw GA Beele H Dermaut LR Tooth transplantation and cryopreservation: state of the art Am J Orthod Dentofacial Orthop 2006 129 691 695 16679211
5 Temmerman L Dermaut LR De Mil M Van Maele G Beele H De Pauw GA Influence of cryopreservation on human periodontal ligament cells in vitro Cell Tissue Bank 2008 9 11 18 17541731

상세보기
6 Kasai M Mukaida T Cryopreservation of animal and human embryos by vitrification Reprod Biomed Online 2004 9 164 170 15333245

상세보기
7 Rall WF Fahy GM Ice-free cryopreservation of mouse embryos at -196 degrees C by vitrification Nature 1985 313 573 575 3969158

상세보기
8 Takahashi T Hirsh A Erbe EF Bross JB Steere RL Williams RJ Vitrification of human monocytes Cryobiology 1986 23 103 115 3698640

상세보기
9 Decherchi P Lammari-Barreault N Cochard P Carin M Rega P Pio J CNS axonal regeneration with peripheral nerve grafts cryopreserved by vitrification: cytological and functional aspects Cryobiology 1997 34 214 239 9160994
10 Song YC Khirabadi BS Lightfoot F Brockbank KG Taylor MJ Vitreous cryopreservation maintains the function of vascular grafts Nat Biotechnol 2000 18 296 299 10700144

상세보기
11 Pegg DE The role of vitrification techniques of cryopreservation in reproductive medicine Hum Fertil (Camb) 2005 8 231 239 16393823

상세보기
12 Fahy GM MacFarlane DR Angell CA Meryman HT Vitrification as an approach to cryopreservation Cryobiology 1984 21 407 426 6467964

상세보기
13 Kasai M Komi JH Takakamo A Tsudera H Sakurai T Machida T A simple method for mouse embryo cryopreservation in a low toxicity vitrification solution, without appreciable loss of viability J Reprod Fertil 1990 89 91 97 2374136

상세보기
14 Pegg DE The history and principles of cryopreservation Semin Reprod Med 2002 20 5 13 11941530

상세보기
15 Isachenko V Lapidus I Isachenko E Krivokharchenko A Kreienberg R Woriedh M Human ovarian tissue vitrification versus conventional freezing: morphological, endocrinological, and molecular biological evaluation Reproduction 2009 138 319 327 19439559

상세보기
16 Armitage WJ Survival of corneal endothelium following exposure to a vitrification solution Cryobiology 1989 26 318 327 2766779

상세보기
17 Bourne WM Nelson LR Human corneal studies with a vitrification solution containing dimethyl sulfoxide, formamide, and 1,2-propanediol Cryobiology 1994 31 522 530 7835051

상세보기
18 Armitage WJ Hall SC Routledge C Recovery of endothelial function after vitrification of cornea at -110 degrees C Invest Ophthalmol Vis Sci 2002 43 2160 2164 12091411
19 Makarevich AV Chrenek P Olexikova L Popelkova M Turanova Z Ostro A Post-thaw survival, cell death and actin cytoskeleton in gene-microinjected rabbit embryos after vitrification Theriogenology 2008 70 675 681 18539321

상세보기

저자의 다른 논문 :

원문 URL 링크

DOI : 10.5051/jpis.2010.40.3.111 [무료]
대한치주과학회 : 저널 > 논문 [무료]
PubMed Central : 저널 > 논문 [무료]
Synapse : 저널
Korea Open Access Journals : 저널
AccessON : 저널

*원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다.

오픈액세스(OA) 유형

GOLD

오픈액세스 학술지에 출판된 논문

내보내기 메뉴

내보내기 구분

파일저장
인쇄
메일전송

구성항목

기본정보
상세정보

관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관

저장형식

Text(ASCII format)
Excel format
RefWorks Direct Export
RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley

메일정보

받는사람 (필수): @
보내는사람 (선택): @
제목
내용: KISTI 검색결과 이메일 서비스

안내

총 건의 자료가 검색되었습니다.

다운받으실 자료의 인덱스를 입력하세요. (1-10,000)

검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다.

데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요)

다운로드 파일은 UTF-8 형태로 저장됩니다.
파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오.

Text(ASCII format)
Excel format

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

AI-Helper ※ AI-Helper는 을 사용합니다.

AI-Helper

안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

연합인증