Gangliosides are a ubiquitous component of the membranes of mammalian cells that have been suggested to play important roles in various cell functions such as cell-cell interaction, adhesion, cell differentiation, growth control and signaling. However, the role that gangliosides play in the immune r...
Gangliosides are a ubiquitous component of the membranes of mammalian cells that have been suggested to play important roles in various cell functions such as cell-cell interaction, adhesion, cell differentiation, growth control and signaling. However, the role that gangliosides play in the immune rejection response in xenotransplantation is not yet clearly understood. In this study, differential expression patterns of gangliosides in HEK293 (human embryonic kidney cells), PK15 (porcine kidney cells), NIH-kd (NIH-mini pig kidney cells, primary cultured) and the cortex, medulla and calyx of the NIH-mini pig kidney were investigated by high-performance thin-layer chromatography (HPTLC). The results revealed that HEK293, PK15 and NIH-kd contained GM3, GM2 and GD3 as major gangliosides. Moreover, GM3, which are the gangliosides of NIH-kd, were expressed at higher levels than HEK293 and PK15. Especially, GT1b were expressed in HEK293 and NIH-kd but not in PK15. Finally, GM1 and GD1a were expressed in NIH-kd, but not in HEK293 or PK15. These results suggest that differential expression patterns of gangliosides from HEK293, PK15 and NIH-kd are related to the immune rejection response in xenotransplantation.
Gangliosides are a ubiquitous component of the membranes of mammalian cells that have been suggested to play important roles in various cell functions such as cell-cell interaction, adhesion, cell differentiation, growth control and signaling. However, the role that gangliosides play in the immune rejection response in xenotransplantation is not yet clearly understood. In this study, differential expression patterns of gangliosides in HEK293 (human embryonic kidney cells), PK15 (porcine kidney cells), NIH-kd (NIH-mini pig kidney cells, primary cultured) and the cortex, medulla and calyx of the NIH-mini pig kidney were investigated by high-performance thin-layer chromatography (HPTLC). The results revealed that HEK293, PK15 and NIH-kd contained GM3, GM2 and GD3 as major gangliosides. Moreover, GM3, which are the gangliosides of NIH-kd, were expressed at higher levels than HEK293 and PK15. Especially, GT1b were expressed in HEK293 and NIH-kd but not in PK15. Finally, GM1 and GD1a were expressed in NIH-kd, but not in HEK293 or PK15. These results suggest that differential expression patterns of gangliosides from HEK293, PK15 and NIH-kd are related to the immune rejection response in xenotransplantation.
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대상 데이터
Eight MAbs specific for one of the following gangliosides, GM3, GM2, GM1, GD3, GDla, GDlb, GTlb and GQlb, were used in this study. HEK293, PK15, NIH-kd and various sections of the NIH-mini pig kidney were appreciably positive for MAbs against GM3, GM2, GM1, GD3, GDlb and GTlb, which correspond to GMR6, MK1-16, GMB16, GMR19, GGR12 and GMR5, respectively.
데이터처리
All data are expressed as the means+ S.E. Statistical differences were determined using a Studenfs unpaired Z-test. Differences were considered significant at p <0.
성능/효과
kidneys. The results suggest that GM3, GM1, GD3 and GTlb are the major gangliosides in the cortex, GM3, GM2, GM1, GD3, GDlb and GTlb are the major gangliosides in the medulla and GM2, GD3, GDlb and GTlb are the major gangliosides in the calyx. Additionally, GM2 and GDlb were not expressed in the cortex, while GM3 and GM1 were not expressed in the calyx.
and PK15 cells. The results suggest that GM3, GM2, GD3 and GTlb are the major gangliosides in HEK293 cells, that GM3, GM2, GM1, GD3, GDlb and GTlb are the major gangliosides in NIH-kd cells and that GM3, GM2 and GD3 are the major gangliosides in PK15 cells. Additionally, GM1 and GDlb were not expressed in the HEK293 cells, while GM1, GDlb and GTlb were not expressed in PK15.
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