최소 단어 이상 선택하여야 합니다.
최대 10 단어까지만 선택 가능합니다.
다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
NTIS 바로가기韓國林學會誌 = Journal of Korean Forest Society, v.99 no.1=no.188, 2010년, pp.125 - 130
김선자 (국립과천과학관) , 박소영 (국립산림과학원 산림생명공학과) , 문흥규 (국립산림과학원 산림생명공학과) , 이위영 (국립산림과학원 산림생명공학과)
The application of bioreactor culture techniques for plant micropropagation is regarded as one of the ways to reduce production cost by scaling-up and automation. In an attempt to optimize mass proliferation systems in Eucalyptus pellita, four types of bioreator systems including temporary immersion...
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
핵심어 | 질문 | 논문에서 추출한 답변 |
---|---|---|
생물반응기를 이용한 액체배양 방법은 기존 고체배양 방법과 비교했을 때 어떤 장점을 가지고 있을까? | 생물반응기를 이용한 액체배양은 기존의 고체배양에 비해 배양규모를 쉽게 늘릴 수 있다(scale-up)는 점에서 무한한 가능성을 가지고 있다. 최근 생물반응기를 이용해 수백 개의 무균 절편체를 일시에 배양하는 대량배양 시스템 (large-scale culture system)이 보고되었다(Hahn과 Paek, 2005; Piao 등, 2003; Shohael 등, 2005). | |
본 연구에서 적용한 배양시스템은 무엇이 있는가? | 배양시스템이 생장에 미치는 영향을 구명하고자 Ebb & Flood 시스템이 가능하도록 7L 규모의 생물반응기를 주문제작하였다. 실험을 위해 적용된 배양시스템은 다음의 4가지이다: 1) TIX(Temporary immersion without net) -절편체를 간헐적으로 액체배지에 잠기게 하는 방법(30분/4시간, 1일 4회), 2) TIN(Temporary immersion with net) - net 위에 치상된 절편체를 간헐적으로 액체배지에 접하는 방법(30분/4시간, 1일 4회), 3) CIN(Continuous immersion with net) - net 위에 치상 된 절편체를 계속 액체배지에 접하게 하는 방법, 4) TINC(Temporary immersion with net following continuous immersion with net) - net 위에 치상 된 절편체를 간헐적(30분/4시간)으로 액체배지에 접하게 한 다음 배양 2주 후 계속 액체배지를 공급하는 방법. | |
열대조림의 중요성이 증가한 이유는 무엇인가? | 최근 기후변화에 따라 탄소배출권을 확보하고 안정적인 목재생산량을 확보하기위해서 인도네시아를 중심으로 한 열대조림의 중요성이 증가하고 있다. 그 일환으로 우수한 형질을 가진 열대 선발목의 클론증식에 대한 관심 또한 날로 증대되고 있다(Park 등, 2008). |
Avila, A., Pereyra S.M., and Arguello, J.A. 1998. Nitrogen concentration and proportion of ${NH_4}^+$ -N affect potato cultivar reaponse in solid and liquid media. HortScience 33: 336-338.
Bandyopadhyay, S., Cane, K., Rasmussen, G., and Hamill, J.D. 1999. Efficient plant regeneration from seedling explants of two commercially important temperate Eucalyptus species- Eucalyptus nitens and E. globulus. Plant Science 140: 189-198.
Chaves, M.M. 1994. Environmental constraints to photosynthesis in ex vitro-90-plants p. 1-18. In: P.J. Lumsden, J.R. Nicholas, and W.J. Davies (eds.). Physiology, growth and development of plants in culture. Kluwer Academic Publishers, Dordrecht, The Netherlands.
Dencso, I. 1987. Factors influencing vitrification of carnation and conifers. Acta Horticulturae 212: 167-176.
Delaporte, K. and Sedgley, M. 2004. Selection and breeding of eucalyptus for ornamental horticulture. Acta Horticulturae 630: 77-84.
Desjardins, Y., Gosselin, A., and Yelle, S. 1987. Acclimatization of in vitro strawberry plantlets under $CO_2$ enriched environment and supplemental lighting. Journal of American Society of Horticulture Science 112: 846-851.
Driver, J.A. and Kuniyuki, A.H. 1984. In vitro propagation of Paradox walnut rootstock. Horticultural Science 19: 507-509.
Eun, J.S. 1998. Acclimatization of in vitro plantlets of Wasabia japonica (Miq.) Matsum. derived from the apical meristem culture. Korean Journal of Plant Tissue Culture. 25: 257-261.
Hahn, E.J. and Paek, K.Y. 2005. Multiplication of Chrysanthemum shoots in bioreactors as affected by culture method and inoculation density of single node stems. Plant Cell, Tissue and Organ Culture 81: 301-306.
Hajari, E., Watt, M.P., Mycock, D.J., and McAlister, B. 2006. Plant regeneration from induced callus of improved Eucalyptus clones. South African Journal of Botany 72: 195-201.
Harwood, C.E., Alloysius D., Pomroy, P., and Robson, K.W. 1997. Early growth and survival of Eucalyptus pellita provenances in a range of tropical environments, compared with E. grandis, E. urophylla and Acasia mangium. New Forests 14: 203-219.
Kim, J.A. and Moon, H.K. 2004. Effect of light-emitting diodes (LED) and ventilation on the in vitro shoot growth of Eucalyptus pellita. Journal of Korean Forrest Society 95: 716-722.
Kim, J.A., Moon, H.K., and Kang, H.D. 2005. Effect of BA and NAA on adventitious bud induction from in vitro germinant Eucalyptus pellita. Korean Journal of Plant Biotechnology 32: 201-207.
Kozai, T., Kubota, C., Zobayed, S.M.A., Nguyen, T.Q., Afreen-Zobayed, F., and Heo, J. 2000. Developing a masspropagation system of woody plants. In: Watanabe, K., Komamine, A. eds. Challenge of plant and agriculture sciences to the crisis of biosphere on the Earth in the 21st century. Georgetown, Landes Biosphere, pp293-306.
Kozai, T. and Kubota, C. 2001. Developing a photoautotrophic micropropagation system for woody plants. Journal of Plant Research 114: 525-537.
Laine, E. and David, A. 1994. Regeneration of plants from leaf explants of micropropagated clonal Eucalyptus grandis. Plant Cell Reports 13: 473-476.
McAlister, B., Finnie, J., Watt, M.P., and Blakeway, F. 2005. Use of the temporary immersion bioreactor system ( $RITA^{\circledR}$ ) for production of commercial Eucalyptus clones in Mondi Forests (SA). Plant Cell, Tissue and Organ Culture 81: 347-358.
McComb, J.A., Bennett, I.J., and Tonkin, C. 1996. In vitro propagation of Eucalyptus species. In: Taji, A.; Williams, R., eds. Tissue culture of Australian plants. Armidale, NSW: University of New England, 112-156.
Merchant, A., Richter, A., Popp, M., and Adams, M. 2006. Targeted metabolite profiling provides a functional link among Eucalyptus taxonomy, physiology and evolution. Phytochemistry 67: 403-408.
Murali, T.P. and Duncan, E.J. 1995. The effects of in vitro gardening using triazoles on growth and acclimatization of banana. Scientia Horticulturae 64: 243-251.
Nugent, G., Chandler, S.F., Phil, W., and Stevenson, T.W. 2001. Adventitious bud induction in Eucalyptus globulus Labill. In Vitro Cellular & Developmental Biology-Plant 37: 388-391.
Paques, M. and Boxus, P.H. 1987. Vitrification: A phenomenon related to tissue water content. Acta Horticulturae 212: 245-252.
Park, S.Y., Moon, H.K., Kim, Y.W., Kim, S.J., and Lee, J.S. 2008. Application of open-type liquid culture for largescale production of mature plus tree of Eucalyptus pellita. Journal of Korean Forestry Society. 97: 650-655.
Paek, K.Y. and Han, B.H. 1989. Physiological, biochemical and morphological characteristics of vitrified shoot regenerated in vitro. Korean Society of Plant Tissue Culture 18: 151-162.
Piao, X.C., Charkrabarty, D., Hahn, E.J., and Paek, K.Y. 2003. A simple method for mass production of potato microtubers using a bioreactor system. Current Science 8: 1129-1232.
Phillips, I.D.I. 1964. Root-shoot hormone relation. Annals of Botany 28: 17-35.
Prasad, V.S.S. and Gupta, E.S.D. 2006. In vitro shoot regeneration of gladiolus in semi-solid agar versus liquid cultures with support systems. Plant Cell, Tissue and Organ Culture 87: 263-271.
Riek, J.D. 1995. Interaction between sucrose uptake and photosynthesis in micropropagated Rosa multiflora L. Ph.D. thesis. Gent University, Belgium.
Schwambach, J., Fadanelli, C., and Fett-Neto, A.G. 2005. Mineral nutrition and adventitious rooting in microcuttings of Eucalyptus globules. Tree Physiology 25: 487-494.
Shohael, A.M., Chakrabarty, D., Yu, K.W., Hahn, E.J., and Paek, K.Y. 2005. Application of bioreactor system for large-scale production of Eleutherococcus sessiliflorus somatic embryos in an air-lift bioreactor and production of eleutherosides. Journal of Biotechnology 120: 228-236.
Takayama, S. and Akita, M. 1994. The types of bioreactors used for shoots and embryos. Plant Cell, Tissue and Organ Culture 39: 147-156.
Tibok, A., Blackhall, N.W., Power, J.B., and Davey, M.R. 1995. Optimized plant regeneration from calls derived from seedling hypocotyls of Eucalyptus urophylla. Plant Science 110: 139-145.
Van Huylenbroeck, J.M., Piqueras, A., and Debergh, P.C. 2000. The evolution of photosynthetic capacity and the antioxidant enzymatic system during acclimatization of micropropagated Calathea plants. Plant Science 155: 59-66.
Warrang, E., Lesney, M.S., and Rockwood, D.J. 1991. Nodule culture and regeneration of Eucalyptus grandis hybrids. Plant Cell Reports 9: 586-589.
Yang, J.C., Chung, J.D., and Chen, Z.Z. 1995. Vegetative propagation of adult Eucalyptus grandis X urophylla and comparison of growth between micropropagated plantlets and rooted cuttings. Plant Cell Reports 15: 170-173.
Zobayed, S.M. A., Afreen F., and Kozai, T. 2001. Physiology of Eucalyptus plantlets grown photoautotrophically in a scaled-up vessel. In Vitro Cellular & Developmental Biology-Plant 37: 807-813.
Zobayed, S.M.A., Afreen, F., Xiao, Y., and Kozai, T. 2004. Recent advancement in research on photoautotrophic micropropagation using large culture vessels with forced ventilation. In Vitro Cellular & Developmental Biology- Plant 40: 450-458.
*원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.