[논문]Investigation of Ectomycorrhizal Fungal Colonization in Pinus thunbergii Seedlings at a Plantation Area in Gangneung, using Morphotyping and Sequencing the rDNA Internal Transcribed Spacer Region

Obase, Keisuke; Cha, Joo-Young; Lee, Jong-Kyu; Lee, Sang-Yong; Lee, Jin-Ho; Chun, Kun-Woo

[국내논문] Investigation of Ectomycorrhizal Fungal Colonization in Pinus thunbergii Seedlings at a Plantation Area in Gangneung, using Morphotyping and Sequencing the rDNA Internal Transcribed Spacer Region 원문보기

韓國林學會誌 = Journal of Korean Forest Society, v.99 no.2=no.189, 2010년, pp.172 - 178

Obase, Keisuke (College of Forest and Environmental Sciences, Kangwon National University) , Cha, Joo-Young (Field Science Center for Northern Biosphere, Hokkaido University) , Lee, Jong-Kyu (College of Forest and Environmental Sciences, Kangwon National University) , Lee, Sang-Yong (College of Forest and Environmental Sciences, Kangwon National University) , Lee, Jin-Ho (College of Forest and Environmental Sciences, Kangwon National University) , Chun, Kun-Woo (College of Forest and Environmental Sciences, Kangwon National University)

Abstract ▼ AI-Helper

The status of ectomycorrhizal (ECM) fungal colonization in Pinus thunbergii seedlings was investigated 2 years after planting in an eastern coastal area of Korea. We established three $10{\times}10$ m plots at a P. thunbergii plantation in Gangneung and sampled lateral roots from 10 seedlings in each plot. ECMs were classified into morphological groups and the number of root tips of each morphotype was counted. In total, 8 ECM morphotypes were observed and fungal species that form each morphotype were identified by sequencing of the internal transcribed spacer (ITS) region of the nuclear rDNA. Suillus granulatus was the most abundant species (44.1-65.7% of relative abundance) in all plots, followed by Tomentella ellisii (14.0-37.8%) and unidentified fungus belonged to Atheliaceae (10.6-20.1%). These 3 fungal species accounted for almost all of the ECM abundance in each plot (94.9-99.8%). The remaining 5 fungal species were uncommon and rare. There was no clear difference in ECM fungal communities among plots. Community structure of ECM fungi in the young P. thunbergii plantation was simple and composed of fungal species that were also observed in mature coastal pine forests.

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가설 설정

1, (g) Tomentella sp. 2, (h) unidentified species belonged to Atheliaceae. Bars indicated 1 mm.

대상 데이터

This study was carried out with the support of “‘Forest Science & Technology Projects (Project No. S210809L010110)'provided by Korea Forest Service. We acknowledge special support from the members of Laboratory of “Forest Resources Development” and “Resources Protection” at Kangwon National University.

데이터처리

Species-accumulation curves were drawn by plotting the mean of the accumulated number of expected species in pooled samples after 50 randomizations without replacement using EstimateS program version 8.0 (Colwell, 2005) for estimating whether sampling effort was enough to describe ECM fungal flora in plots. Estimated measurement of ECM fungal species richness using bootstrap, Jackknife 1 and Jackknife 2 estimators and similarity of fungal flora using Morishita-Horn index between plots were calculated by using EstimateS program version 8.
0 (Colwell, 2005) for estimating whether sampling effort was enough to describe ECM fungal flora in plots. Estimated measurement of ECM fungal species richness using bootstrap, Jackknife 1 and Jackknife 2 estimators and similarity of fungal flora using Morishita-Horn index between plots were calculated by using EstimateS program version 8.0 (Colwell, 2005). ECM fungal diversity (Shannon's and Simpson's index) was also estimated using EstimateS program version 8.

이론/모형

(Daejeon, South Korea). Fungal sequences were compared with the GenBank database at the National Center for Biotechnology Information (NCBI: http://www.ncbi. nlm.nih.gov) and UNITE (http://unite.ut.ee), using the nucleotide-nucleotide basic local alignment search tool algorithm (BLAST program). Sequences were aligned with closely matched reference sequences and submitted to neighbor-joining analysis at NCBI for taxonomic interpretation at the species, genus or family level.

성능/효과

and Tomentella ellisii (Sacc.) Jülich & Stalpers) and 3 fungal species (Inocybe sp., Tomentella sp. 1 and Tomentella sp. 2) to species and genus level by matching sequences with those of known sporocarps.
These 3 fungal species were observed in 27 to 29 out of 30 soil samples. The remaining 5 fungal species were uncommon and showed low relative abundance (less than 0.1 to 3.8%) and frequencies (1 to 3 out of 30 soil samples).

후속연구

Therefore, it should be advantageous to inoculate site-adaptable and effective fungal strains on seedlings before planting in plantation site in coastal areas. Further investigations on the effects of naturally inhabiting ECM fungi on the establishment of P. thunbergii seedlings in coastal areas are needed for understanding what kinds of ECM fungal strains are effective for establishment of seedlings and applicable in reforestation program in coastal areas.

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