[논문]매실 혼합물이 DSS로 유도된 염증성 장질환 동물모델의 면역조절에 미치는 활성

김해란; 이보람; 임경직; 신흥묵; 임병우

doi:10.7783/kjmcs.2011.19.1.016

매실 혼합물이 DSS로 유도된 염증성 장질환 동물모델의 면역조절에 미치는 활성
Anti-Inflammatory Effects of Prunus mume Mixture in Colitis Induced by Dextran Sodium Sulfate 원문보기

韓國藥用作物學會誌 = Korean journal of medicinal crop science, v.19 no.1, 2011년, pp.16 - 23

김해란 (건국대학교 충주캠퍼스 의료생명대학 응용생명과학과) , 이보람 (건국대학교 충주캠퍼스 의료생명대학 응용생명과학과) , 임경직 (충주 고등학교) , (건국대학교 충주캠퍼스 의료생명대학 응용생명과학과) , 신흥묵 (동국대학교 한의대 생리학과) , 임병우 (건국대학교 충주캠퍼스 의료생명대학 응용생명과학과)

Abstract ▼ AI-Helper

This study was conducted to investigate the anti-inflammatory effects of Pruns mume, Schisandra chinensis, Chaenomeles sinensis-- Prunus mume mixtrue (PM) treatment on colitis induced in mice by dextran sodium sulfate (DSS) treatment. A total of 25 male BALB/c mice (average weight $20.7\;{\pm}\; 1.6 \;g$) were divided into 5 treatment groups and fed a commercial diet (A), PM administration (B), commercial diet + induced colitis by DSS (C), PM administration + induced colitis by DSS (D) and sulfasalazine + induced colitis by DSS (E). We found that PM treatment (D) and sulfasalazine (E) decreased the expression of $TNF-{\alpha}$ and COX-2 compared to the DSS-induced colitis group (C). The expression of IL-4, STAT6, $IFN-{\gamma}$, STAT1 was decreased in group D and group E compared to the colitis group (C), COX-2 and STAT1 were more decreased in group D. The serum IgE levels decreased in the PM treatment groups (C and D) compared to the non-PM treatment groups (A and B) although there was no significant difference between the PM treatment groups. It is notable that a therapeutic application of the PM extracts ameliorated DSS-induced colitis in mice.

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문제 정의

The purpose of this study was to investigate the immunological effect of dietary PM treatment in mice with inflammatory bowel disease (IBD) caused by DSS treatment. We demonstrate that PM mixture has anti-inflammatory effects in DSS-inducedmice.
Thus, the purpose of this study was to evaluate the ability of PM mixture to inhibit IBD, especially colitis in the mice model.

제안 방법

Group C was administrated PM (50 ㎎/㎏), group D was administrated PM (50 ㎎/㎏) with colitis induced by DSS, and group E was administrated sulfasalazine (3 ㎎/㎏) with colitis induced by DSS (Ye and Lim, 2010). After 7 days of DSS administration, the mice resumed drinking plain water, and mice were administered oral doses of PM (50 ㎎/㎏) for 15 days.
The surface molecules of T cells were observed in spleens by the mAb directed to CD4, CD8 and a control isotope conjugated with fluorochromes (FITC, PE). All antibodies were purchased from BD Pharmingen (San Diego, CA, USA), and sample analyses were carried out on a FACS Vantage flow cytometer (BD Bioscience, San Jose, CA, USA). Absolute cell numbers were calculated by multiplying the flow cytometry percentage by the total numbers of viable cells.
Because weight gain and total food intake can be indicators of intestinal health, we monitored body weight and food intake for each of the 5 groups of mice, before and after treatment (Table 1). The average body weight of the DSS-induced group was less than the average body weight of the control group and the PM group has higher average body weight than the DSS-induced group.

대상 데이터

Diet and sterilized water were provided ad libitum throughout the experiment. After 1 week of adaptation, 25 animals were randomly divided into 5 groups. Group A was fed a commercial diet, and group B was fed a commercial diet with colitis induced by DSS.
Dulbecco's Modified Eagle's medium (DMEM), and 3-(4,5 dimethythiazol2-yl)-2,5-diphenytetrazoleum (MTT) were obtained from Wako Chemical Co. (Tokyo, Japan).

데이터처리

Differences between the means of the individual groups were assessed by one-way analysis of variance with Duncan’s multiple range tests.

이론/모형

Antibodies IgE and IgA were measured by using sandwich ELISA methods, as reported previously by Lim et al. (2005).

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