세계적 멸종위기종인 따오기(Nipponia nippon)는 2008년 10월에 중국에서 1쌍이 도입된 후 한국최초로 인공번식에 성공하였다. 본 연구는 따오기의 sex-related gene과 Chromodomain Helicase DNA Binding Protein gene (CHD gene)을 가지고 polymerase chain reaction (PCR)을 수행하여 새로 태어난 따오기 유조의 성별을 확인하고자 하였다. 본 연구에서는 따오기의 성별 확인을 위해 PCR후 제한효소의 처리 방법과 P2과 P8를 이용한 PCR 방법을 실시하였을 때 더 정확한 결과가 나타남을 알 수 있었다. 그리고 CHD gene의 염기서열을 선행연구와 비교해 본 결과, 암컷의 염기서열에서 1~2 base pairs 차이가 나타남을 알 수 있었다.
세계적 멸종위기종인 따오기(Nipponia nippon)는 2008년 10월에 중국에서 1쌍이 도입된 후 한국최초로 인공번식에 성공하였다. 본 연구는 따오기의 sex-related gene과 Chromodomain Helicase DNA Binding Protein gene (CHD gene)을 가지고 polymerase chain reaction (PCR)을 수행하여 새로 태어난 따오기 유조의 성별을 확인하고자 하였다. 본 연구에서는 따오기의 성별 확인을 위해 PCR후 제한효소의 처리 방법과 P2과 P8를 이용한 PCR 방법을 실시하였을 때 더 정확한 결과가 나타남을 알 수 있었다. 그리고 CHD gene의 염기서열을 선행연구와 비교해 본 결과, 암컷의 염기서열에서 1~2 base pairs 차이가 나타남을 알 수 있었다.
In October 2008, a pair of Crested ibis Nipponia nippon, an endangered avian species in the world, was donated to Korea from China. They have since been the subject of a successful program to incubate chicks for the first time in South Korea. This study was carried out to determine the sex of chicks...
In October 2008, a pair of Crested ibis Nipponia nippon, an endangered avian species in the world, was donated to Korea from China. They have since been the subject of a successful program to incubate chicks for the first time in South Korea. This study was carried out to determine the sex of chicks from the Crested ibis through polymerase chain reaction (PCR) using the sex-related gene and the chromodomain helicase DNA binding protein (CHD) gene. The result of the CHD gene, which was used with a single set of primers and a restriction enzyme treatment after the PCR process, was more accurate in identifying the gender of the Crested ibis. In addition, we compared the CHD gene sequences with the previously reported sequences and found 1~2 different bases between females (CI2, CI4, CI5, and CI6) than in studies previously reporting female sequences.
In October 2008, a pair of Crested ibis Nipponia nippon, an endangered avian species in the world, was donated to Korea from China. They have since been the subject of a successful program to incubate chicks for the first time in South Korea. This study was carried out to determine the sex of chicks from the Crested ibis through polymerase chain reaction (PCR) using the sex-related gene and the chromodomain helicase DNA binding protein (CHD) gene. The result of the CHD gene, which was used with a single set of primers and a restriction enzyme treatment after the PCR process, was more accurate in identifying the gender of the Crested ibis. In addition, we compared the CHD gene sequences with the previously reported sequences and found 1~2 different bases between females (CI2, CI4, CI5, and CI6) than in studies previously reporting female sequences.
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가설 설정
A: CI2, CI4, CI5, and CI6, which showed a band at about 307 bp, were considered female, and CI1 and CI3, which did not show a band, were considered male. B: The band at about 262 bp was amplified from both the male and female. PCR products contain two primers.
제안 방법
The aim of current study was to determine the gender of each young progeny from the parents mentioned above by detecting the sex-related gene and chromodomain helicase DNA binding protein (CHD) gene, and by analyzing the sequences of the amplicons via polymerase chain reaction (PCR) of the Crested ibis for the restoration of the species in South Korea. In addition, this study recommend which is the more accurate and efficient method to identifying the gender of Crested ibis.
The aim of current study was to determine the gender of each young progeny from the parents mentioned above by detecting the sex-related gene and chromodomain helicase DNA binding protein (CHD) gene, and by analyzing the sequences of the amplicons via polymerase chain reaction (PCR) of the Crested ibis for the restoration of the species in South Korea. In addition, this study recommend which is the more accurate and efficient method to identifying the gender of Crested ibis.
In October 2008, a pair of Crested ibis donated from China had successfully incubated chicks for the first time in South Korea. This study used the Sex-related gene [9] and CHD gene [7] in order to identify the gender of Crested ibis, which were the first incubated chicks in South Korea. The first method, a one-off PCR was able to identify the gender of Crested ibis, was very simple, but in this study the results did not always have an expected outcome.
대상 데이터
The bird materials for the present study included a pair of parent Crested ibis (CI1: adult male, CI2: adult female) introduced from China and their four chicks (CI3, CI4, CI5, and CI6, respectively) domestically born in South Korea, all of which were bred at the Upo Crested ibis Restoration Center in captivity. Tissue specimens were collected from the feathers of the parent and chicks.
성능/효과
1A). By the result, the W chromosome-specific DNA band did not occur in the mature male Crested ibis (CI1), while the mature female (CI2) was showed the band. The first chick (CI3) was equal to the paternal parent CI1, hence the second (CI4), third (CI5) and fourth chick (CI6) were as same as the maternal parent CI2.
This study used the Sex-related gene [9] and CHD gene [7] in order to identify the gender of Crested ibis, which were the first incubated chicks in South Korea. The first method, a one-off PCR was able to identify the gender of Crested ibis, was very simple, but in this study the results did not always have an expected outcome.
The first chick (CI3) was equal to the paternal parent CI1, hence the second (CI4), third (CI5) and fourth chick (CI6) were as same as the maternal parent CI2. Therefore, it was considered that CI3 is male progeny, while CI4, CI5, and CI6, implicate CI2, were considered to be females. Moreover, the PCR was performed with the new single set of primers, designed from the W chromosome-specific DNA sequences of Crested ibis, but it was amplified from both the male and female (Fig.
후속연구
The DNA sequences of males (CI1 and CI3) and M1 consisted with each other, however the sequences of females (CI2, CI4, CI5, and CI6) and F1 were different in 1~2 bases. It is believed that this study will provide the basic data for the genetic diversity of the Korean population of the Crested ibis and for establishing an effective species restoration strategy. The third method, which used the CHD gene with P2 and P8, was quite a bit inconvenient too.
참고문헌 (15)
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