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난소제거된 Shiba 염소에서 다른 농도의 Progesterone 처치에 의한 FSH Surge 및 Pulse 분비에 미치는 영향
Patterns of Pulsatile and Surge Modes of Follicle-Stimulating Hormone Treated with Different Progesterone Levels in Ovariectomized Goats 원문보기

Journal of veterinary clinics = 한국임상수의학회지, v.28 no.2 = no.79, 2011년, pp.225 - 231  

Kim, Seung-Joon (경북대학교 수의과대학 수의임상번식학교실) ,  Tanaka, Tomomi (일본 동경농공대학 수의과대학 수의임상번식학교실) ,  Kamomae, Hideo (일본 동경농공대학 수의과대학 수의임상번식학교실)

초록
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본 연구의 목적은 FSH 분비 조절에 있어서 각각 다른 progesterone (P) 농도가 Pulse 또는 Surge 형태의 분비 패턴에 어떤 영향을 미치는가를 탐구한다. 선행된 연구에서 LH surge는 난포기 (follicular phase)에서 보이는 P 농도에서만 발현되었고 황체기(luteal phase) 또는 아황체기(subluteal phase) P 농도에서는 완전히 억제되었다. 또한 LH pulse 분비는 황체기 P농도에서만 시간 의존적으로 감소되었으나 난포기 또는 아황체기 농도에서는 높은 빈도로 유지되었다. 즉 아황체기 P농도 (스트레스, 황체형성부전에서 발생)는 LH surge는 억제시키나 LH pulse 조절에는 아무런 영향을 마치지 않았다. 이 결과를 바탕으로 FSH surge 및 pulse 분비패턴을 알아보기 위해 난소가 제거된 Shiba 염소를 이용하여 인위적으로 난포기, 아황체기, 기능성황체기의 P 농도 및 estradiol(E) 농도를 유도하였다. 스테로이드 호르몬 농도의 변화를 알아보기 위해 매일 혈액을 채취하였으며, LH pulse 용 혈액은 10분 간격으로 8시간 동안 Day 0, Day 3, Day 7 (Day 0: P packet 이식 직전)에 연속채혈을 실시하고, LH surge 분비패턴은 Day 8에 16시간 estradiol를 3 ${\mu}g/h$ 농도로 주사하고, 52시간 동안 2시간 간격으로 채혈을 실시했다. 그 결과 유도된 P 농도는 염소의 정상적 발정주기에서 볼 수 있는 난포기(<0.1 ng/ml), 아황체기($1.1{\pm}0.1$ ng/ml), 황체기 ($3.3{\pm0.1}$ ng/ml) 수준의 농도로 유도되었다. FSH pulse 분비는 3그룹에서 모두 높은 빈도로 유지되었고, FSH surge는 LH surge와 같은 시간대에서 난포기 그룹에서만 확인되었다. 본 연구의 결과는 FSH pulse는 LH pulse에서 보이는 progesterone에 의한 억제 효과와 다르게 나타났으며, 이러한 결과는 FSH 분비 조절에 있어서 LH 분비와 다른 기전 및 요소들이 관여하는 것으로 판단되었다.

Abstract AI-Helper 아이콘AI-Helper

The objective of the present study was to determine the progesterone levels that effects on the pulsatile and surge modes of FSH secretion. In previous studies we have shown that LH surge occurred in the follicular levels of progesterone, whereas there was no surge mode secretion of LH in either the...

주제어

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제안 방법

  • The goats were ovariectomized at least 3 mo before the start of the experiment and were maintained outdoors with a diet of hay-cubes given twice daily and water given ad libitum. Basically, the animals in this study were divided into three groups in a 3 x 3 Latin square design (finally, n = 4 or 5 for each group), and at least 4 wk were allowed to elapse after each treat­ ment. All animals were housed and loose-tied at the start of the experiment.
  • Fig 2. Patterns of pulsatile follicle-stimulating hormone (FSH) secretion in representative animals from the control (Non-P) group (No. 14, left panels), low progesterone (Low P) group (No. 18, miolc panels) and high progesterone (High P) group (No. 9, right panels) on the day just before the progesterone treatment (Day 0, upper), and on the third day (Day 3, middle) and seventh day (Day 7, bottom) of progesterone treatment. Arrowheads indicate the FSH pulses.
  • The purpose of the present study was to determine the effects of different progesterone levels similar to follicular phase, sub­ luteal phase, and luteal phase, on secretion patterns of FSH in ovariectomized goats treated with subcutaneous implants of progesterone.

대상 데이터

  • Long-term ovariectomized Shiba goats (n = 9) weighing bet­ ween 20 and 30 kg (mean ± SD, 24.6 ± 1.9 kg) were used. Shiba goats are nonseasonal breeders under natural daylight (25).

데이터처리

  • Data were analyzed using the Scheflfe method of analysis of variance with the StatView computer program (StatView 4.5; Abacus Concepts Inc., Berkeley, CA). During the implantation of steroids, one-way analysis of variance was also used to deter­ mine the significance of differences among the mean concentra­ tions of progesterone and estradiol among the three groups.
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