$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

조직배양 유래 자생나리 소인경의 온탕 및 저온처리가 맹아 및 비대에 미치는 효과

Effects of Hot Water and Chilling Treatments of Bulblets Propagated by Tissue Culture on Sprouting and Bulb Development in Korean Native Lilies

초록

본 연구에서는 관상가치가 높은 5종의 자생나리에 있어서 조직배양을 통해 획득한 소인경의 휴면타파 및 비대를 위한 저온 및 온탕처리 효과를 구명하기 위해 실시되었다. 솔나리(L. cernuum), 섬말나리(L. hansonii), 민섬말나리(L. hansonii for. mutatum), 중나리(L. leichtlinii), 하늘말나리(L. tsingtauense)의 소인경을 $4^{\circ}C$에서 0, 4, 8주 저온처리하거나 $35^{\circ}C$의 증류수 또는 $100mg{\cdot}L^{-1}$$GA_3$$GA_{4+7}$ 용액으로 2시간 온탕처리를 하였는데, 저온과 온탕처리는 처리의 선후를 달리하면서 순차적으로 처리한 후 $20^{\circ}C$의 유리온실에서 맹아 및 비대 양상을 조사하였다. 자생나리 5종 모두 기내 소인경은 모두 휴면에 돌입하여 저온 또는 온탕처리 없이는 맹아하지 않았다. 휴면타파를 위한 온탕처리에는 5종 모두 $GA_{4+7}$이 55-96%까지 맹아를 유도한 반면, 증류수나 $GA_3$ 용액은 거의 효과가 없었다. 솔나리와 중나리는 4주간의 저온처리만으로도 50-70%의 맹아를 유도한 반면, 섬말나리, 민섬말나리의 맹아에는 8주간의 저온처리가 필요했다. 온탕과 저온의 연속처리는 맹아 유도에 상승효과를 주었는데, 솔나리와 섬말나리에서 $GA_{4+7}$ 온탕처리 후 4주 저온처리한 것이 역순의 처리보다 맹아를 35-45% 촉진시켰으며, 맹아소요일수도 3-9일까지 앞당겼다. 구근의 비대는 맹아가 촉진된 처리구에서 증가된 경향이었다. 신초는 솔나리에서만 출현하였는데, $GA_{4+7}$ 온탕처리와 저온처리에서 효과적이었으며, 특히 $GA_{4+7}$ 온탕처리 후 저온 8주 처리에서 신초 출현률이 가장 높게 나타났다. 이상의 결과를 종합할 때, 맹아 및 인경비대를 위해 가장 효과적인 휴면타파법은 섬말나리, 민섬말나리와 중나리의 경우에는 $GA_{4+7}$온탕처리 후 4주 저온처리였고, 솔나리와 하늘말나리에서는 $GA_{4+7}$ 온탕처리 후 8주 저온처리구였다.

Abstract

This study was carried out to investigate the effects of hot solution soaking and chilling treatment on sprouting and enlargement of bulblets obtained through in vitro culture in Korean native lilies with ornamental values. In vitro bulblets of Lilium cernuum, L. hansonii, L. hansonii for. mutatum, L. leichtlinii, and L. tsingtauense were soaked in distilled water or 100 $mg{\cdot}L^{-1}$ $GA_3$ and $GA_{4+7}$ solution maintained at $35^{\circ}C$ for two hours (hot water treatment) and/or exposed at $4^{\circ}C$ for 0, 4, or 8 weeks (chilling treatment) and then planted in plastic trays filled with media and grown in a greenhouse at $20^{\circ}C$ and under 16 h photoperiod. In all species, no bulblet propagated by tissue culture sprouted without chilling or hot water treatment due to dormancy. For dormancy breaking, $GA_{4+7}$ hot solution treatment increasing sprouting by 55-96%, whereas distilled water or GA was not effective in sprouting. Chilling treatment for 4 weeks induced sprouting by 50-70% in L. cernuum and L. leichtlinii, whereas 8 weeks was needed for sprouting of L. hansonii and L. hansonii for. mutatum. Combined treatment of hot water and chilling treatments synergistically promoted sprouting. Especially, in L. cernuum and L. hansonii, $GA_{4+7}$ hot solution soaking prior to chilling for 4 weeks promoted sprouting by 35-45% compared with the reverse order. Enlargement of bulblets resulted from increase in fresh weight and diameter was promoted by the treatments that increased the sprouting percentage of bulblets. Only in L. cernuum, shoots emerged from bulblets soaked in hot $GA_{4+7}$ solution or chilled at $4^{\circ}C$ and shoot emergence rate was highest in bulblets soaked in hot $GA_{4+7}$ solution and then chilled for 8 weeks. From these results, the most effective method for bulblet sprouting and enlargement was to soak in hot $GA_{4+7}$ solution and then chill for 4 weeks in L. hansonii, hansonii for. mutatum, and leichtlinii, and to soak in hot $GA_{4+7}$ solution and then chill for 4 weeks in L. cernuum and tsingtauense.

참고문헌 (27)

  1. Choi, S.T. 1983. Effects of low temperature and hot water treatment to the dormant bulbs on the leaf emergences of bulblets during scale propagation of Easter lily (Lilium longiflorum Thunb.). J. Kor. Soc. Hort. Sci. 24:42-48. 
  2. Choi, S.T. 1998. Cut-flower production of bulbous plants. Kor. J. Hort. Ind. Sci. 2:63-74. 
  3. Higgins, W.S. and D.P. Stimart. 1990. Influence of in vitro generation temperature and post-in vitro cold storage duration on growth response of Lilium longiflorum bulblets. J. Amer. Soc. Hort. Sci. 115:930-933. 
  4. Isabelle, D., P. Annie, and G.J. Klerk. 1990. The development of dormancy in bulblet of Lilium speciosm generated in vitro. II. The effect of temperature. Physiol. Plant 80:431-436. 
  5. Kamerbeek, G.A., T.C.M. Beijersbergen, and P.K. Schenk. 1972. Dormancy in bulbs and corms. Proc. 18th Int. Hort. Congr. 5:233-239. 
  6. Kim, J.Y., S.T. Choi, M.S. Roh, and T.S. Ko. 1996. Production and detection of virus-free lily plants by shoot tip culture and virazole treatment of bulbils. J. Kor. Soc. Hort. Sci. 37:64-69. 
  7. Kim, K.S. 1991. The effect of growth regulators, temperature and sucrose on the dormancy in Lillium speciosum bulblets cultured in vitro. Kor. J. Plant Tissue Culture 18:103-111. 
  8. Kim, K.W. 1999. Control and manipulation of dormancy in Lilium bulblets regenerated in vitro. Proceedings of the First Joint Symposium between Hokkaido Univ. and Yeungnam Univ., Yeungnam Univ. Press, Korea p. 136-142. 
  9. Kim, K.W. and A.A. De Hertogh. 1997. Tissue culture of ornamental flowering bulbs (geophytes). Hort. Rev. 18:87-169. 
  10. Kim, K.W., J.S. Kim, E.Y. Kim, J.D. Choi, and K.I. Park. 1998. Effects of culture conditions on dormant status of in vitro regenerated Lilium Oriental Hybrid bulblets. J. Kor. Soc. Hort. Sci. 39:641-646. 
  11. Kim, K.W., K.A. Kim, and M.S. Byun. 1999. Physiological changes before and after breaking dormancy of Lilium hansonii bulblets regenerated in vitro. J. Kor. Soc. Hort. Sci. 40:751-754. 
  12. Kim, K.W., S.Y. Moon, and S.J. Eum. 2003. Shortening dormancy breaking period by $GA_{4+7}$ addition in hot-water treatment of Korea native Lilium bulblets regenerated in vitro. J. Kor. Soc. Hort. Sci. 44:744-747. 
  13. Kim, K.W., J.H. Shin, J.D. Choi, and J.A Kim. 2002. Difference between species of shoot emergence ability from lily bulblets regenerated in vitro. J. Kor. Soc. Hort. Sci. 43:226-230. 
  14. Lighty, R.W. 1969. The lilies of Korea. The Lily Year Book, Royal Hort. Soc., London. 
  15. Lin, W.C., H.F. Willins, and M. Angell. 1975. Exogenous gibberellins and abscisic acid effects on growth and development of Lillium longiflorum. J. Amer. Soc. Hort. Sci. 100:9-16. 
  16. Matsuo, E. and K. Arisumi. 1979. Differences in chilling effects in shoot emergence from the bulb and on leaf emergence from the scale bulblet in Lilium longiflorum Thunb. HortScience 14:68-69. 
  17. Matsuo, E., K. Arisumi, and H. Kawashima. 1982. Cultural practices influencing premature daughter leaf and/or shoot emergence in scale-propagated Easter lily. HortScience 17:196-198. 
  18. Niimi, Y., Y. Endo, and E. Arisaka. 1988. Effect of chilling and $GA_{3}$ treatments on breaking dormancy in Lilium rubellum Baker bulblets cultures in vitro. J. Jpn. Soc. Hort. Sci. 57: 250-257. 
  19. Park, K.I., J.D. Choi, M.S. Byun, J.J. Choi, K.H. Kwon, and K.W. Kim. 2000. Introduction and proliferation of callus in Liliium Oriental Hybrids in vitro. J. Kor. Soc. Hort. Sci. 41:297-300. 
  20. Stimart, D.P. and P.D. Ascher. 1981. Foliar emergence from bulblets of Lilium longiflorum Thunb. as related to in vitro generation temperatures. J. Amer. Soc. Hort. Sci. 106:446-450. 
  21. Stimart, D.P., P.D. Ascher, and H.F. Wilkins. 1982. Overcoming dormancy in Lilium longiflorum bulblets produced in tissue culture. J. Amer. Soc. Hort. Sci. 107:101-107. 
  22. Stimart, D.P., P.D. Ascher, and H.F. Wilkins. 1983. Axis elongation from tissue-culture-generated bulblets of Lilium longiflorum Thunb. J. Amer. Soc. Hort. Sci. 108:99-101. 
  23. Takayama, S. and M. Misawa. 1980. Differentiation in Lilium bulbscales grown in vitro. Effect of activated charcoal, physiological age of bulbs and sucrose concentrations on differentiation and scale leaf formation in vitro. Physiol. Plant 48: 121-125. 
  24. Takayama, T., T. Toyomasu, H. Tamane, N. Murofushi, and H. Yajima. 1993. Identification of gibberellins and abscisic acid in bulbs of Lilium ${\times}$ elegans Thunb. and their quantitative changes during cold treatment and the subsequent cultivation. J. Jpn. Soc. Hort. Sci. 62:189-196. 
  25. Tsukamoto, Y. 1971. Change of endogenous growth substances in Easter lily as affected by cooling. Acta Hort. 23:75-81. 
  26. Woo, J.H., Y.Y. Han, Y.G. Sim, H.S. Lee, K.B. Choi, J.D. Choi, and K.W. Kim. 2000. Effect of growth regulators and culture method on shoot formation from microscale in Lilium Oriental Hybrids 'Casa Blanca'. J. Kor. Soc. Hort. Sci. 41:297-300. 
  27. Yae, B.W., B.H. Han, and D.H. Goo. 2001. Dormancy breaking and in vitro growth of in vitro bulblets in Lilium Oriental hybrid 'Casa Blanca'. J. Kor. Soc. Hort. Sci. 42:99-102. 

이 논문을 인용한 문헌 (0)

  1. 이 논문을 인용한 문헌 없음

DOI 인용 스타일