Tyrosinase is one of the important enzymes in the mammalian melanin synthesis. In the process of melanin synthesis, tyrosine is oxidized to DOPA (3,4-dihydroxyphenylalanine), and DOPA is further oxidized to dopaquinone. Tyrosinase is an enzyme catalyzing this oxidation of tyrosine, so chemicals that...
Tyrosinase is one of the important enzymes in the mammalian melanin synthesis. In the process of melanin synthesis, tyrosine is oxidized to DOPA (3,4-dihydroxyphenylalanine), and DOPA is further oxidized to dopaquinone. Tyrosinase is an enzyme catalyzing this oxidation of tyrosine, so chemicals that inhibit the activity of tyrosinase can be used as skin whitening agents. In this study, we isolated five constituents from the 80% MeOH extract of Morus bombycis cortex by bioactivity-guided fractionation. We performed mushroom tyrosinase inhibition assay. As a result, 7,2',4'-trihydroxyflavanone (1), 2',4',2,4,-tetrahydroxychalcone (2), and oxyresveratrol (3) showed the more potent inhibitory effect compared to kojic acid, a well-known skin whitening agent with antityrosinase effect. Moracinoside M (4) and moracin M-3'-O-${\beta}$-D-glucopyranoside (5) also showed the moderate tyrosinase inhibitory activities.
Tyrosinase is one of the important enzymes in the mammalian melanin synthesis. In the process of melanin synthesis, tyrosine is oxidized to DOPA (3,4-dihydroxyphenylalanine), and DOPA is further oxidized to dopaquinone. Tyrosinase is an enzyme catalyzing this oxidation of tyrosine, so chemicals that inhibit the activity of tyrosinase can be used as skin whitening agents. In this study, we isolated five constituents from the 80% MeOH extract of Morus bombycis cortex by bioactivity-guided fractionation. We performed mushroom tyrosinase inhibition assay. As a result, 7,2',4'-trihydroxyflavanone (1), 2',4',2,4,-tetrahydroxychalcone (2), and oxyresveratrol (3) showed the more potent inhibitory effect compared to kojic acid, a well-known skin whitening agent with antityrosinase effect. Moracinoside M (4) and moracin M-3'-O-${\beta}$-D-glucopyranoside (5) also showed the moderate tyrosinase inhibitory activities.
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제안 방법
For the isolation of bio-active compounds from Morus bombycis cortex, the inhibitory effects of total extract and the fractions (including n-Hexane, EtOAc, BuOH and water fractions) of this plant on tyrosinase were evaluated. As shown in Fig.
, 2002). In this study, we isolated a number of constituents from Morus bombycis cortex by bioactivity-guided fractionation, and evaluated the inhibitory effects of the isolated compounds on mushroom tyrosinase.
Ultrasonication was performed using a Branson 5200 sonicator. MPLC was performed using Teledyne CombiFLASH RETRIEVE using Teledyne Redisep Rf C18 reverse phase columns. Preparative HPLC was performed using HITACHI L-7100 pump with HITACHI L-7420 UV detector and Phenomenex Hemini C18 5 µm column (150 × 10 mm).
대상 데이터
Preparative HPLC was performed using HITACHI L-7100 pump with HITACHI L-7420 UV detector and Phenomenex Hemini C18 5 µm column (150 × 10 mm). ESI MS data were obtained on Agilent 1100 series LC/MSD trap LC/MS. NMR spectra were recorded on JEOL GSX 400 Spectrometer (400 MHz), JEOL LA 300 Spectrometer (300 MHz), and Bruker AMX 500 Spectrometer (500 MHz).
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