[논문]Effects of Chenopodium album Linne on Gastritis and Gastric Cancer Cell Growth

Kim, Pitna; Jeong, Choon-Sik

doi:10.4062/biomolther.2011.19.4.487

Effects of Chenopodium album Linne on Gastritis and Gastric Cancer Cell Growth 원문보기

Biomolecules & therapeutics, v.19 no.4, 2011년, pp.487 - 492

Kim, Pitna (College of Pharmacy, Duksung Women's University) , Jeong, Choon-Sik (College of Pharmacy, Duksung Women's University)

Abstract ▼ AI-Helper

In our previous study, we investigated Chenopodium album Linne (CAL) ethanol extract and its fractions on anti-gastritic actions using the HCl/ethanol and indomethacin induced gastric lesion model and Helicobacter pylori (H. pylori). Based on the results, butanol fraction was most effective among fractions obtained from CAL. This study aims to elucidate the mechanisms of butanol fraction, and betaine as a constituent of the butanol fraction, on gastritis and anti-gastric cancer cell growth. First, we examined antioxidant properties using hydrogen peroxide and superoxide radical, and we found that butanol fraction and betaine may be good antioxidants. Second, cytotoxicity was assessed by measuring cell viability and 4,6-diamidino-2-phenylinodole dihydrochloride (DAPI) staining of human gastric cancer cells (AGS cells). We also examined the relationship between the cytotoxicity and intracellular $Ca^{2+}$ signaling mechanism. The butanol fraction demonstrated cell viability 71.49% at the concentration of 100 ${\mu}g/ml$ and increased intracellular $Ca^{2+}$ concentration in a dose dependent manner. Finally, we observed the mucus content as a defensive factor and gastric secretion as an aggressive factor, and found that the mucus content noticeably increased when treated with butanol fraction and betaine and gastric secretion decreased when treated with betaine in vivo study. From these results, we suggest that CAL butanol fraction and betaine may have protective effects on gastritis.

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문제 정의

We have found that butanol fraction was the most effective in inhibiting HCl/ethanol and indomethacin induced gastric lesions among fractions obtained from CAL leaves. The aim of this study is to elucidate the protective mechanisms of butanol fraction and betaine as a constituent (Fig. 1). Betaine is widely distributed in micro-organisms, plants, and animals (Craig, 2004) and also called trimethylglycine, glycine betaine, lycin or oxyneurine.

제안 방법

And then, we investigated cytotoxicity on butanol fraction in gastric cancer cells. First, we performed the MTT assay using AGS human gastric cancer cells, and visualized it through the DAPI staining method. And to elucidate the relationship between cytotoxicity and concentration of intracellular Ca²⁺, we measured the intracellular Ca²⁺infl ux.
In this study, we chose CAL, as it known for its protective function of stomach and liver as a folk remedy. In the hydrogen peroxide scavenging activity test, both butanol fraction and betaine showed higher scavenging activities than L-ascorbic acid, which well-known as an antioxidant.
The aim of this study was to investigate the mechanism of anti-gastritis and anti-gastric cancer cell growth of the CAL butanol fraction and betaine by measuring their anti-oxidant activities, cytotoxicity to the gastric cancer cells, intracellular Ca²⁺concentration, the mucus content, and gastric secretion.

대상 데이터

AGS gastric cancer cells were obtained from the Korean Cell Line Bank (KCLB, Seoul, Korea). These cells were cultured with RPMI-1640 containing 10% FBS, penicillin (100 units/ml), and streptomycin (100 lg/ml) in a 5% CO₂ humidified incubator at 37℃.
All experiments were performed in triplicate. Data was analyzed using the Student’s t-test.
Dimethyl sulfoxide (DMSO), fura-2AM, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), trypan blue, probenecid, dantrolene sodium, sodium bicarbonate, dimethyl sulfoxide, 4,6-diamidino-2-phenylinodole dihydrochloride (DAPI), cimetidine were obtained from Sigma (Sigma-Aldrich Inc., MO, USA). Cell culture media and reagents, including Hank's balanced salt solution, RPMI 1640, fetal bovine serum (FBS), penicillin/streptomycin, and trypsin-EDTA were obtained from GIBCO (Invitrogen Inc.

데이터처리

Data was analyzed using the Student’s t-test.

이론/모형

So, we examined the relationship between the cytotoxic action and intracellular Ca²⁺ signaling mechanisms. For this, we measured the change of intracellular Ca²⁺ concentration using Fura-2 fluorescence technique. Gastric ulcers and gastritis seem to be caused by over-secretion of gastric juice.
Internal Ca²⁺ release mechanism mediates increased intracellular Ca²⁺ concentration. Intracellular Ca²⁺ concentration was assessed by Fura-2 fl uorescence technique. The data represent intracellular Ca²⁺changes with time.
Intracellular Ca²⁺ concentrations were measured using a modifi ed version of the method described by Kim et al. (2003). Aliquots of the AGS cells were washed in PBS.
The superoxide radical scavenging activity was determined according to the method of Nishikimi (1972). 0.

참고문헌 (24)

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상세보기
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Effects of Chenopodium album Linne on Gastritis and Gastric Cancer Cell Growth 원문보기

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Effects of Chenopodium album Linne on Gastritis and Gastric Cancer Cell Growth 원문보기

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