In order to facilitate the quality control of the fruits of Crataegus pinnatifida, a simple, accurate and reliable HPLC method was developed for the simultaneous determination of the three bioactive compounds: chlorogenic acid (1), rutin (2), and hyperin (3), which were selected as the chemical mark...
In order to facilitate the quality control of the fruits of Crataegus pinnatifida, a simple, accurate and reliable HPLC method was developed for the simultaneous determination of the three bioactive compounds: chlorogenic acid (1), rutin (2), and hyperin (3), which were selected as the chemical markers of C. pinnatifida. Separation was achieved on an Agilent Eclipse XDB-C18 column with a gradient solvent system of 0.1% trifluoroacetic acid aqueous-acetonitrile at a flow-rate of 1.0 mL/min and detected at 254nm. All three calibration curves showed good linearity ($R^2$ > 0.998). The recoveries of three marker compounds were in the range of 94.87~111.52 %. The contents of chlorogenic acid (1), rutin (2), and hyperin (3) of the fruits of C. pinnatifida collected from 23 district markets in Korea, Japan, and China were 0.16~0.65 mg/g, 0.07~1.24 mg/g, and 0.03~0.62 mg/g, respectively. The results demonstrated that this method is simple and reliable for the quality control of the fruits of C. pinnatifida.
In order to facilitate the quality control of the fruits of Crataegus pinnatifida, a simple, accurate and reliable HPLC method was developed for the simultaneous determination of the three bioactive compounds: chlorogenic acid (1), rutin (2), and hyperin (3), which were selected as the chemical markers of C. pinnatifida. Separation was achieved on an Agilent Eclipse XDB-C18 column with a gradient solvent system of 0.1% trifluoroacetic acid aqueous-acetonitrile at a flow-rate of 1.0 mL/min and detected at 254nm. All three calibration curves showed good linearity ($R^2$ > 0.998). The recoveries of three marker compounds were in the range of 94.87~111.52 %. The contents of chlorogenic acid (1), rutin (2), and hyperin (3) of the fruits of C. pinnatifida collected from 23 district markets in Korea, Japan, and China were 0.16~0.65 mg/g, 0.07~1.24 mg/g, and 0.03~0.62 mg/g, respectively. The results demonstrated that this method is simple and reliable for the quality control of the fruits of C. pinnatifida.
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제안 방법
In order to achieve a complete extraction of the studied components from the fruits of C. pinnatifida four solvent systems, including methanol, 70% methanol, ethanol, and 70% ethanol, were tested. The extraction efficiencies of all of the components from each of the solvent extraction systems were obtained and compared.
pinnatifida. In this study, a simple, accurate and reliable analytical method for simultaneous quantification of the three active components in the fruits of C. pinnatifida were developed using high-performance liquid chromatography. Separation was achieved on an Agilent Eclipse XDB-C18 column (5 µm, 150 × 4.
Recovery test was used to evaluate the accuracy of the assay. Accurate amounts of the three standards were added into a sample of C.
The chromatographic separation of analyses was performed carried out on an Agilent Eclipse XD8-C18 (Agilent Technologies, USA; 5 µm, 4.6 × 150 mm) performed at ambient temperature using a MetaTherm (Varian, USA).
The established analytical method was then applied to quantitatively analyze three compounds 1 - 3 in various samples of C. pinnatifida, using the regression equation as described above. Their contents were summarized in Table 4.
대상 데이터
HPLC grade MeOH and acetonitrile were purchased from Merck K GaA (Darmstadt, Germany). Distilled and deionized water were obtained from the central instrument center (Catholic University of Daegu, Daegu, Korea) and used throughout the study. Trifluoroacetic acid (TFA) was obtained from Sigma-Aldrich (Missouri, USA).
The chromatographic system for quantitative analysis consisted of a 306 pump (Gilson, USA), 811C dynamic mixer (Gilson, USA), UV/VIS-156 detector (Gilson, USA), 231 XL sample injector (Gilson, USA), and GILSON UniPoint data processor (Gilson, USA). The chromatographic separation of analyses was performed carried out on an Agilent Eclipse XD8-C18 (Agilent Technologies, USA; 5 µm, 4.
pinnatifida were collected from Korea, Japan, and China markets: 11D1001 (purchased from Jecheon, cultivated in Korea), 11D1002 (purchased from Jecheon, cultivated in Korea), 11D1003 (purchased from Sancheong, cultivated in Korea), 11D1004 (purchased from Gyeongju, cultivated in Korea), 11D1005 (purchased from Gyeongju, cultivated in Korea), 11D1006 (purchased from Gyeongju, cultivated in Korea), 11D1007 (purchased from Jecheon, cultivated in Korea), 11D1008 (purchased from Gyeongju, cultivated in Korea), 11D1009 (purchased from Gyeongju, cultivated in Korea), 11D1010 (purchased from Gyeongju, cultivated in Korea), 11D1011 (purchased from Ulsan, cultivated in Korea), 11D1012 (purchased from Gyeongju, cultivated in Korea), 11D1013 (purchased from Gyeongju, cultivated in Korea), 11D1014 (purchased from Gyeongju, cultivated in Korea), 11D1015 (purchased from Gyeongju, cultivated in Korea), 11D1016 (purchased from Ulsan, cultivated in Korea), 11D1017 (purchased from Tokyo, cultivated in China), 11D1018 (purchased from Sandong, cultivated in China), 11D1019 (purchased from Seomseo, cultivated in China), 11D1020 (purchased from Sandong, cultivated in China), 11D1021 (purchased from Habuk, cultivated in China), 11D1022 (purchased from Habuk, cultivated in China), and 11D1023 (purchased from Oklim, cultivated in China). The origin of sample was identified by Prof. Je Hyun Lee, Dongguk University, Korea and voucher specimens were deposited in Catholic University of Daegu, Korea.
Distilled and deionized water were obtained from the central instrument center (Catholic University of Daegu, Daegu, Korea) and used throughout the study. Trifluoroacetic acid (TFA) was obtained from Sigma-Aldrich (Missouri, USA). Others solvents and reagents were of analytical grade.
이론/모형
pinnatifida, which was quantified previously. The mixture was extracted and compounds using the above-established method. Each sample was analyzed in triplicate.
성능/효과
The extraction efficiencies of all of the components from each of the solvent extraction systems were obtained and compared. The results indicated that, for chlorogenic acid (1), rutin (2), and hyperin (3), the 70% methanol and 70% ethanol solvent systems were demonstrated to be more efficient than the methanol and ethanol solvent systems (Table 3). From compounds, aqueous solvent system was exhibited to be more efficient than organic solvent system.
참고문헌 (18)
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