[논문]개 말초혈액 다형핵백혈구의 유주활성에 있어 fucoidan의 효과

전춘진; 김수현; 김성수; 강지훈; 양만표

[국내논문] 개 말초혈액 다형핵백혈구의 유주활성에 있어 fucoidan의 효과
Fucoidan Upregulates Chemotactic Activity of Canine Peripheral Blood Polymorphonuclear Cells Through Interleukin-8 from Peripheral Blood Mononuclear Cells in vitro 원문보기

Journal of veterinary clinics = 한국임상수의학회지, v.29 no.3, 2012년, pp.207 - 212

전춘진 (충북대학교 수의과대학) , 김수현 (충북대학교 수의과대학) , 김성수 (충북대학교 수의과대학) , 강지훈 (충북대학교 수의과대학) , 양만표 (충북대학교 수의과대학)

초록
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개 말초혈액 다형핵백혈구(PMNs)의 유주활성에 대한 fucoidan의 면역증효과를 검토하였다. Fucoidan 그 자체는 PMNs에 대해 직접적인 유주활성 효과를 보이지 않았다. 그러나 recombinant IL-8에 대한 PMNs의 유주활성과 유사하게 fucoidan으로 배양한 말초혈액 단핵구세포(PBMCs)의 배양상층액은 PMNs에 대해 유주활성을 증가시켰다. 또한, fucoidan으로 배양한 PBMCs의 배양상층액에 대한 PMNs의 유주활성 증가효과는 anti-IL-8 pAb를 처리했을 때 억제되었다. PBMCs 배양 상층액 속의 IL-8의 양을 정량한 결과 fucoidan무처치 대조군에 비해 증가하였다. 이상의 결과로부터, fucoidan은 개 PMNs의 유주능에 대하여 면역증가 작용을 가지고 있으며, 이것은 fucoidan의 자극에 의해 PBMCs에서 생산되어 분비되는 IL-8에 의해 나타나는 것으로 사료되었다.

Abstract ▼ AI-Helper

Fucoidan has been shown to enhance immune function. The objective of this study was to examine the in vitro effect of fucoidan on the chamotactic activity of canine peripheral blood polymorphonuclear cells (PMNs). The chemotactic activity of PMNs was evaluated by method of a modified Boyden chamber assay. The amount of interleukin (IL)-8 in the culture supernatants from peripheral blood mononuclear cells (PBMCs) treated with fucoidan was determined by means of ELISA. Fucoidan itself could not have chemoattract effects for PMNs. However, the chemotaxis of PMNs was remarkably enhanced by culture supernatant from PBMCs treated with fucoidan. Similarly, it was also increased by recombinant canine (rc) IL-8. These chemotactic activities of PMNs were inhibited by addition of anti-rcIL-8 polyclonal antibody (pAb). The amount of IL-8 in the culture supernatant from PBMCs was shown to increase upon treatment of fucoidan as compared with that of untreated PBMCs culture supernatant. These results suggest that fucoidan upregulates the chemotaxis of PMNs, which is mainly mediated by IL-8 released from fucoidanstimulated PBMCs.

주제어

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문제 정의

The aim of this study is to examine the in vitro effect of fucoidan on the chemotaxis of PMNs. We examined the migration distances of PMNs chemoattracted by culture supernatant of PBMCs treated with fucoidan.

가설 설정

It induces lysosomal enzyme release from neutrophils, expression of adhesion molecules on neutrophils (11). Consequently, we hypothesized that the soluble factor(s) in the culture supernatant of fucoidan-treated PBMCs may be associated with IL-8-like chemotactic factor. We tested the effect of IL-8 on the chemotactic activity of canine PMNs.

제안 방법

To examine whether the enhanced chemotactic acitvity of PMNs to the culture supernatant from PBMCs treated with fucoidan is due to IL-8, the neutralization effect of anti-rcIL-8 pAb on chemotactic activity of PMNs in response to culture supernatant of PBMCs was examined. The enhanced chemotactic activity of PMNs to culture supernatant from PBMCs treated with fucoidan was inhibited by addition of anti-rcIL-8 pAb at concentration of 10 and 100 µg/ml in a dose-dependent manner when compared with that of culture supernatant (75%) from PBMCs treated with fucoidan (p < 0.

대상 데이터

For the blood donor, we used clinically healthy five male Beagle dogs. Their mean age was 10 months old.

데이터처리

One-way analysis of variance (ANOVA) was used to investigate differences between the control and treatment groups, followed by Dunnett's post hoc test.
One-way analysis of variance (ANOVA) was used to investigate differences between the control and treatment groups, followed by Dunnett's post hoc test. Comparison of two groups was made by t-test. P value of under 0.
One-way ANOVA was used to investigate differences between control and treatments, followed by a Dunnett’s test.
One-way ANOVA was used to investigate differences between rIL-8 treated groups and vehicle control, followed by a Dunnett’s test.
One-way ANOVA was used to investigate differences between control and treatments, followed by a Dunnett’s test. And, comparison of two groups was made by t-test. Data represent the means ± SD (n = 3).
One-way ANOVA was used to investigate differences between fucoidan-treated PBMCs culture supernatant (75%) alone and other treatments, followed by a Dunnett’s test.
One-way ANOVA was used to investigate differences between rIL-8 (10 nM) treated group and other treatments, followed by a Dunnett’s test.

성능/효과

To the clinical application, the co-administration of fucoidan will be able to augment the host defense in animals with diseases. In conclusion, this study suggested that fucoidan-treated PBMCs culture supernatant enhances the chemotactic activity of canine PMNs, potentially through the increased IL-8.

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