참나무시들음 병원균 Raffaelea quercus-mongolicae에 대한 항균미생물 분리 Antifungal Property of Microorganisms against Korea Oak Wilt Pathogen, Raffaelea quercus-mongolicae원문보기
Raffaelea quercus-mongolicae에 대한 항균작용을 하는 미생물을 분리하기 위하여 200개의 균주를 분리하였으며 이 중 SG 1-9, 1-12와 SG 2-8, 2-10, 2-17 5개의 균주에서 항균활성을 확인하였다. 5개의 균주는 확인을 위하여 16S rDNA 염기서열 분석을 하였으며, SG 1-9는 Streptomyces cinnamoneus와 98%의 homology가 SG 1-12는 Burkholderia cepacia와 98% homology가 같은 것으로 나타났다. 또한 SG 2-8은 Streptomyces fradiae와 99%의 homology를 SG 2-10은 Staphylococcus epidermidis와 97%를 SG 2-17은 Staphylococcus epidermidis와 99%의 homology를 나타내었다. 위 5개의 균주 중 활성이 가장 강한 SG 2-17의 유기용매추출물과 단백질추출물을 분리하여 활성을 조사하였으며 유기용매추출물에서 강한 활성을 확인하였다. 3개의 유기용매 중 benzene 추출물이 가장 높은 Raffaelea quercus-mongolicae의 균사성장을 억제하였다.
Raffaelea quercus-mongolicae에 대한 항균작용을 하는 미생물을 분리하기 위하여 200개의 균주를 분리하였으며 이 중 SG 1-9, 1-12와 SG 2-8, 2-10, 2-17 5개의 균주에서 항균활성을 확인하였다. 5개의 균주는 확인을 위하여 16S rDNA 염기서열 분석을 하였으며, SG 1-9는 Streptomyces cinnamoneus와 98%의 homology가 SG 1-12는 Burkholderia cepacia와 98% homology가 같은 것으로 나타났다. 또한 SG 2-8은 Streptomyces fradiae와 99%의 homology를 SG 2-10은 Staphylococcus epidermidis와 97%를 SG 2-17은 Staphylococcus epidermidis와 99%의 homology를 나타내었다. 위 5개의 균주 중 활성이 가장 강한 SG 2-17의 유기용매추출물과 단백질추출물을 분리하여 활성을 조사하였으며 유기용매추출물에서 강한 활성을 확인하였다. 3개의 유기용매 중 benzene 추출물이 가장 높은 Raffaelea quercus-mongolicae의 균사성장을 억제하였다.
Five strains out of 200 candidate strains (SG 1-9, 1-12, SG 2-8, 2-10, and 2-17) were selected to determine their antifungal activity against Raffaelea quercus-mongolicae. The 16S rDNA sequences of the five strains were determined by sequencing analysis and analyzed by the homology of the blast prog...
Five strains out of 200 candidate strains (SG 1-9, 1-12, SG 2-8, 2-10, and 2-17) were selected to determine their antifungal activity against Raffaelea quercus-mongolicae. The 16S rDNA sequences of the five strains were determined by sequencing analysis and analyzed by the homology of the blast program at NCBI. The homology search showed that SG 1-9 and 1-12 had a 98% homology with Streptomyces cinnamoneus and 98% homology with Burkholderia cepacia, while SG 2-8, 2-10, and 2-17 had a 99% homology with Streptomyces fradiae, a 97% homology with Staphylococcus epidermidis, and a 99% homology with Staphylococcus epidermidis. Out of the five selected strains, organic extract and protein extracts of SG2-17 strain broth were employed to determine antifungal activity against Raffaelea quercus-mongolicae. The organic extract exhibited antifungal activity, but the protein extracts did not demonstrate such an activity. Three organic solvents, butanol, benzene, and ethyl acetate, were also used for determination of antifungal activities. The activity measurements revealed that benzene extract possessed the greatest inhibitory effect on the growth of Raffaelea quercus-mongolicae, with the next highest being butanol extract, and ethyl acetate extract being the lowest.
Five strains out of 200 candidate strains (SG 1-9, 1-12, SG 2-8, 2-10, and 2-17) were selected to determine their antifungal activity against Raffaelea quercus-mongolicae. The 16S rDNA sequences of the five strains were determined by sequencing analysis and analyzed by the homology of the blast program at NCBI. The homology search showed that SG 1-9 and 1-12 had a 98% homology with Streptomyces cinnamoneus and 98% homology with Burkholderia cepacia, while SG 2-8, 2-10, and 2-17 had a 99% homology with Streptomyces fradiae, a 97% homology with Staphylococcus epidermidis, and a 99% homology with Staphylococcus epidermidis. Out of the five selected strains, organic extract and protein extracts of SG2-17 strain broth were employed to determine antifungal activity against Raffaelea quercus-mongolicae. The organic extract exhibited antifungal activity, but the protein extracts did not demonstrate such an activity. Three organic solvents, butanol, benzene, and ethyl acetate, were also used for determination of antifungal activities. The activity measurements revealed that benzene extract possessed the greatest inhibitory effect on the growth of Raffaelea quercus-mongolicae, with the next highest being butanol extract, and ethyl acetate extract being the lowest.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
제안 방법
2) for 2 hours at 4℃, following two times washing with distilled water. Samples were dehydrated in a graded ethanol series (30,50, 70, 80, 90, 100, 100, and 100%), and critical point dried in liquid CO2 using the Balzers CPD 010 (Balzers Instruments, Liechtenstein) after the treatment of isoamyl acetate for 15 minutes two times, and mounted on aluminum stubs,and coated with gold using the Polaron SEM Coating UnitE5100 (Thermo VG Scientific, Beverly, MA).
성능/효과
Three organic solvents, butanol, benzene, and ethyl acetate,were used for the determination. The determination showed that benzene extract was highest in the inhibition of growth of Raffaelea quercus-mongolicae, and next was butanol extract, and ethyl acetate extract was lowest (Table 1, Fig. 2).
The 16S rDNA sequences were analyzed by the homology of BLAST program at NCBI. The homology search showed that SG 1-9 and 1-12 had 98% homology with Streptomyces cinnamoneus and 98% homology with Burkholderia cepacia, SG 2-8, 2-10, and 2-17 had 99% homology with Streptomyces fradiae, 97% homology with Staphylococcus epidermidis, and 99% homology with Staphylococcus epidermidis.
The another attempt was to use the antagonistic properties of mushroom strain against Korean oak wilt pathogen, Raffaelea quercus-mongolicae[1]. The results showed that the species of mushrooms living on different host plants had the antagonistic properties of the pathogen. As a result of the above attempt,microbes have been used as good resources for biological control to inhibit the growth of pathogen.
참고문헌 (9)
Jeon, S. M., K. H. Ka, and K. H. Kim. 2010. Antagonistic properties of mushroom strains to Korean oak wilt pathogen, Raffaelea quercus-mongolicae. Kor J. Mycol. 38: 62-68.
Keiko, K. 2001. Responses of Quercus sapwood to infection with the pathogenic fungus of a new wilt disease vectored by the ambrosia beetle Platypus quercivorus. J. Wood Sci. 47: 425-429.
Kim, S. W., K. S. Kim, K. Lamsal, Y. J. Kim, S. B. Kim, M. Jung, S. J. Sim, H. S. Kim, S. J. Chang, J. K. Kim, and Y. S. Lee. 2009. An in vitro study of the antifungal effect of silver nanoparticles on oak wilt pathogen Raffaelea sp. J. Microbial. Biotechnol. 9: 760-764.
Kinuura, H. 2002. Relative dominance of the model fungus, Raffaelea sp., in the mycangium and proventriculus in relation to adult stages of the oak playpodid beetle, Platypus quercivorus (Coleoptera; Platypodidae). J. For. Res. 7: 7-12.
Lee, S. H., K. H. Kim, S. C. Shin, J. Kim, and Y. S. Yi. 2007. Soil Microorganisms against Cryphonectria parasitica. J. Appl. Biol. Chem. 50: 173-174.
Seo, S. T., K. H. Kim, S. H. Lee, Y. N. Kwon, C. H. Shin, H. J. Kim, and S. Y. Lee. 2010. Genotypic characterization of oak wilt pathogen Raffaelea quercus-mongolicae and R. quercivora strains. Res. Plant Dis. 16: 219-223
Watve, M. G., R. Tickoo, M. M. Jog, and B. D. Bhole. 2001). "How many antibiotics are produced by the genus Streptomyces?". Arch. Microbiol. 176: 386-390.
Williams, S. T., M. Goodfellow, and G. Alderson. 1989. Genus Streptomyces pp. 2452-2492. In Bergey's Manual of Systematic Bacteriology. vol. 4.
Williams, S.T. and F. L. Davis. 1967. Use of a scanning electron microscope for the examination of actinomycetes. Journal of General Microbiology. 48: 171-177.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.