전복내장추출물의 항산화 및 human dermal fibroblasts에 대한 항피부노화 효과 Anti-oxidant and Anti-skin-aging Effects of Abalone Viscera Extracts in Human Dermal Fibroblasts원문보기
전복가공부산물인 내장의 이용가치를 향상시키고자 열수추출, 에탄올추출 등의 방법으로 유효성분을 추출하고 항산화 및 항피부노화 활성을 측정하였다. DPPH 라디칼소거능 분석결과 Tris-HCl 추출물은 $58.60{\pm}0.88%$으로 높은 라디칼소거능을 보였으며, 다음으로 에탄올추출물은 $55.40{\pm}0.62%$의 라디칼소거능을 보였다. Anti-elastase활성을 분석한 결과 에탄올추출물이 다른 시험구에 비하여 현저하게 높은 활성을 보였다. 전복내장추출물의 세포독성을 확인하기 위하여 human dermal fibroblast 세포에 대하여 MTT 시험결과 세포독성은 전혀 나타나지 않았다. 에탄올 추출물의 농도를 달리하여 human dermal fibroblast 세포에 대해pro-collagen type I 생합성능시험결과 10.0 ${\mu}g/mL$에서 $705.30{\pm}3.06$ ng/mL로 retinoic acid$453.60{\pm}2.82$ ng/mL보다 우수한 결과를 보였다. MMP-1 활성은 10 ${\mu}g/mL$에서 $45.30{\pm}0.80$ ng/mL를 보여 control의 $62.37{\pm}0.56$ ng/mL 보다 감소정도가 낮았다. 본 연구는 전복내장추출물의 항산화와 항노화활성에 관하여 최초로 시도되었으며 향후 전복내장을 이용한 다양한 연구의 기초자료로서 중요한 의미가 있다.
전복가공부산물인 내장의 이용가치를 향상시키고자 열수추출, 에탄올추출 등의 방법으로 유효성분을 추출하고 항산화 및 항피부노화 활성을 측정하였다. DPPH 라디칼소거능 분석결과 Tris-HCl 추출물은 $58.60{\pm}0.88%$으로 높은 라디칼소거능을 보였으며, 다음으로 에탄올추출물은 $55.40{\pm}0.62%$의 라디칼소거능을 보였다. Anti-elastase활성을 분석한 결과 에탄올추출물이 다른 시험구에 비하여 현저하게 높은 활성을 보였다. 전복내장추출물의 세포독성을 확인하기 위하여 human dermal fibroblast 세포에 대하여 MTT 시험결과 세포독성은 전혀 나타나지 않았다. 에탄올 추출물의 농도를 달리하여 human dermal fibroblast 세포에 대해pro-collagen type I 생합성능시험결과 10.0 ${\mu}g/mL$에서 $705.30{\pm}3.06$ ng/mL로 retinoic acid $453.60{\pm}2.82$ ng/mL보다 우수한 결과를 보였다. MMP-1 활성은 10 ${\mu}g/mL$에서 $45.30{\pm}0.80$ ng/mL를 보여 control의 $62.37{\pm}0.56$ ng/mL 보다 감소정도가 낮았다. 본 연구는 전복내장추출물의 항산화와 항노화활성에 관하여 최초로 시도되었으며 향후 전복내장을 이용한 다양한 연구의 기초자료로서 중요한 의미가 있다.
In this study, the anti-oxidant and anti-elastase activities of four abalone viscera extracts were investigated to screen the most promising extract. This extract was further studied in terms of its anti-skin-aging properties. In the DPPH-scavenging assay, the Tris-HCl extract showed a $58.60{\...
In this study, the anti-oxidant and anti-elastase activities of four abalone viscera extracts were investigated to screen the most promising extract. This extract was further studied in terms of its anti-skin-aging properties. In the DPPH-scavenging assay, the Tris-HCl extract showed a $58.60{\pm}0.88%$ radical-scavenging activity, which was followed closely by the ethanol extract that had a $55.40{\pm}0.62%$ scavenging activity. In the anti-elastase assay, however, the ethanol extract showed the significantly highest elastase inhibition activity. Furthermore, none of the extracts had a harmful effect on the human dermal fibroblast, as revealed in the MTT assay. In the cell study, the effect of the ethanol extract at various concentrations on the human dermal fibroblast was investigated. At the 10 ${\mu}g/mL$ concentration, the ethanol extract boosted the pro-collagen type I synthesis to $705.30{\pm}3.06$ ng/mL and reduced the MMP-1 to $54.30{\pm}0.80$ ng/mL, which was considered the optimum concentration. This is the first study that focused on the anti-oxidant and anti-skin-aging effects of abalone viscera extract. Its results may provide fundamental data for further study.
In this study, the anti-oxidant and anti-elastase activities of four abalone viscera extracts were investigated to screen the most promising extract. This extract was further studied in terms of its anti-skin-aging properties. In the DPPH-scavenging assay, the Tris-HCl extract showed a $58.60{\pm}0.88%$ radical-scavenging activity, which was followed closely by the ethanol extract that had a $55.40{\pm}0.62%$ scavenging activity. In the anti-elastase assay, however, the ethanol extract showed the significantly highest elastase inhibition activity. Furthermore, none of the extracts had a harmful effect on the human dermal fibroblast, as revealed in the MTT assay. In the cell study, the effect of the ethanol extract at various concentrations on the human dermal fibroblast was investigated. At the 10 ${\mu}g/mL$ concentration, the ethanol extract boosted the pro-collagen type I synthesis to $705.30{\pm}3.06$ ng/mL and reduced the MMP-1 to $54.30{\pm}0.80$ ng/mL, which was considered the optimum concentration. This is the first study that focused on the anti-oxidant and anti-skin-aging effects of abalone viscera extract. Its results may provide fundamental data for further study.
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문제 정의
The effect of abalone viscera ethanol extract on the pro-collagen type I synthesis ability of human dermal fibroblast was examined in this study. Human dermal fibroblast was treated with distilled water and retinoic acid (at the concentration of 0.
But the difference between the four extracts was not significant except the MeOH extract at 50 μg/mL which showed nearly the same cell viability compared with the control group. The study suggested that all the abalone extracts had no harmful effect on the human dermal fibroblast in the present experimental conditions. Since human dermal fibroblast is the main producer of collagen, increased dermal fibroblast may alleviate the skin aging.
Moreover, the ethanol extract stimulated the Pro-Collagen type I synthesis and inhibited MMP-1 activity in the human dermal fibroblast. This study may provide fundamental data about the anti-oxidant and anti-skin-aging ability of abalone.
제안 방법
The MMP-1 activity was examined with the MMP-1 immunoassay kit (R&D Systems, Minneapolis, MN, USA).
The experimental group was treated with abalone viscera ethanol at concentrations of 3.125, 8.250, 10.0, 12.5, 25.0, 50.0, 100.0, 200.0 μg/mL.
대상 데이터
Fresh abalone (Haliotis Discus HannaiIno) was bought from local aquatic market in Wando-gun, Jeonnam-do, which was harvested in the local mariculture farm in February 2011. After abalone was shucked and eviscerated, the viscera was gathered and homogenized and stored at -20℃ before frozen at -70℃ for 48 hr.
The human dermal fibroblast, CCD-1064SK (ATCC CRL-2076), was obtained from American Type Culture Collection (Manassas, VA, USA) and cultured in Iscove's Modified Dulbecco's Midium containing 10% fetal bovine serum (FBS).
데이터처리
To verify the statistical significance of the studied parameters, data are expressed as means and standard deviation (mean ± SD). Comparisons were made using the one-way analysis of variance (ANOVA). The p-values <0.
이론/모형
Moisture, crude protein, crude fat, and ash were determined by the AOAC official methods. Crude protein was estimated from the total nitrogen by multiplying by 6.
The cytotoxicity of extracts was evaluatedwith 3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazoli-um bromide (MTT) assay. The human dermal fibroblasts were trypsinized, centrifuged at 200×g for 5 min and resuspended in Iscove's Modified Dulbecco's Midium.
The cytotoxicity of the four abalone viscera extracts was evaluated by the MTT assay. The viability of human dermal fibroblast which was treated with various abalone viscera extracts was presented in the Table.
성능/효과
The result showed at 10.000 μg/mL the ethanol extract could significantly increase the pro-collagen type I synthesis and inhibit the MMP-1 activity.
후속연구
And from four extracts, we selected one which showed better result and employed it in the cell tests. With all the data obtained in this study, we hope to demonstrate the anti-skin-aging properties of abalone viscera and contribute to further exploitation in this field.
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