[논문]파라-쿠마린산의 자외선B 차단 효과

송교선; 부용출

doi:10.15230/scsk.2012.38.3.263

파라-쿠마린산의 자외선B 차단 효과
UVB-Shielding Effects of para-Coumaric Acid 원문보기

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.38 no.3, 2012년, pp.263 - 273

송교선 (경북대학교 의학전문대학원 분자의학교실 세포기질연구소) , 부용출 (경북대학교 의학전문대학원 분자의학교실 세포기질연구소)

초록
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최근 연구에서 잠재적 피부 색소침착 경감제로서 파라-쿠마린산(PCA)의 주목되는 특성이 발견되었다. 본 연구의 목적은 이 물질의 자외선 차단 효과를 탐구하는 것이다. 자외선에 노출된 HaCaT 세포의 생존율에 대한 PCA의 영향을 in vitro에서 조사하고, 자외선 흡수 스펙트럼이 유사한 방향성 아미노산 대사물들의 작용과 비교하였다. In vivo시험으로는 PCA 크림(1.5 %)과 크림 베이스를 SKH-1 무모 쥐의 등 피부에 도포하고 UVB에 의한 염증 반응으로 나타나는 피부색(홍반) 및 두께 변화(부종)를 측정하였다. 크림 도포-자외선 조사는 2일 간격으로 총 12회 반복하였다. HaCaT 세포를 UVB에 노출시켰을 때 광량 의존적으로 세포 생존율이 감소하였다. 자외선 노출(10 mJ $cm^{-2}$)에 의한 세포 생존율감소는 100 ${\mu}M$의 PCA, cinnamic acid, urocanic acid, 그리고 indole acrylic acid에 의해 각각 39, 27, 39, 31 %가 억제되었다. 무모 쥐의 등 부위에 도포된 PCA크림(10 ${\mu}g\;cm^{-2}$)은 자외선(150 mJ $cm^{-2}$)-노출 피부의 색 지수, 즉 $L^*$, $a^*$ 및 $b^*$ 값, 그리고 두께의 변화를 각각 59, 50, 58, 53 %씩 억제하였다. 본 연구의 결과는 PCA의 멜라닌 생성 억제 작용을 밝힌 선행 연구와 함께 PCA가 자외선에 노출된 피부의 색소 이상 침착과 염증 반응을 막아줄 수 있음을 시사하였다.

Abstract ▼ AI-Helper

Recent studies have uncovered attractive properties of para-coumaric acid (PCA) as a potential skin hywhitening agent. The purpose of the current study was to examine its UVB-shielding effects. Effects of PCA on the viability of HaCaT cells exposed to UVB were assessed in vitro in comparison with other aromatic amino acid metabolites that have similar UV absorption spectra. For in vivo test, PCA cream (1.5 %) and cream base were topically applied to the dorsal skin of SKH-1 hairless mice and the inflammatory responses due to UVB exposure were monitored by changes in skin color (erythema) and thickness (edema). The cream application-UVB exposure regimen was repeated every other day for a total of 12 sessions. When HaCaT cells were irradiated with UVB, there was a dose-dependent decline in cell viability. The cell viability decline due to UVB exposure (10 mJ $cm^{-2}$) was significantly prevented by 100 ${\mu}M$ PCA, cinnamic acid, urocanic acid, or indole acrylic acid by 39, 27, 39, or 31 %, respectively. Topical application of PCA cream onto the dorsal skin of hairless mice (10 ${\mu}g\;cm^{-2}$) attenuated the changes of color parameters, $L^*$, $a^*$, $b^*$ values, and thickness of the UVB (150 mJ $cm^{-2}$)-exposed skin by 59, 50, 58, and 53 %, respectively. The current study, together with the previous studies that demonstrated the antimelanogenic effects of PCA, suggested that PCA may prevent not only dyspigmentation but also inflammatory reactions in the UVB-exposed skin.

주제어

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문제 정의

However, UVB-shielding effect of PCA has been relatively less investigated compared to its antimelanogenic effect. Thus, the present study was aimed at the assessment of the UVB-shielding effect of PCA in vitro in HaCaT human keratinocytes and in vivo in SKH-1 hairless mice.

제안 방법

Figure 3. Transmittance spectra of PCA cream and cream base used in this study. The transmittance spectra of cream base (A) and PCA cream (B) diluted in ethanol were recorded in the 200 ∼ 400 nm range.

대상 데이터

Figure 4. A mouse-housing device used in this study. The housing device has roof windows for the irradiation of dorsal skin sites of the mice (A).
After an acclimation period, mice were randomly divided into 3 groups of 10 ∼ 11 animals.
Animal experiments were performed in accordance with the guideline of Kyungpook National University, intramural Animal Use and care Committee. Five-week-old female SKH-1 hairless mice were purchased from Orient Bio, INC. (Gyeonggido, Korea). The animals were maintained under controlled environmental conditions (23 ± 1 ℃, 55 ± 5 % humidity, 12 h light/dark cycle) with free access to water and an ad libitum standard laboratory diet (Superfeed Co, Wonju, Kangwon, Korea).
This device was designed for convenient and accurate UVB irradiation on the dorsal skin areas of the mice without anesthesia usage. The device was constructed with Plexiglas (3 mm thick), by putting together one roof board, one ground board, and 6 lateral boards, hence allowing simultaneous housing of five mice. For UVB exposure of the mice dorsal skin areas, 5 windows (20 mm × 20 mm) were drilled through the roof board.

데이터처리

Data were presented as the means ± SEM of three or more independent experiments. Significant differences among the groups were determined by one-way ANOVA. Duncan’s multiple-range test was done if differences were identified between the groups at p < 0.

성능/효과

The present study demonstrated that PCA attenuates UVB-induced injury at cellular levels as effectively as UCA and the other aromatic amino acid metabolites tested (Figure 2). This study also demonstrated that PCA topically applied in the form of cream can have beneficial effects mitigating UVB-induced inflammatory responses and injury (Figures 5 and 6), probably due to its strong absorption of UVB radiation (Figures 1 and 3). Together with previous studies that demonstrated the antimelanogenic effects of PCA by in vitro and in vivo experiments [10-16], it is now suggested that PCA may attenuate not only dyspigmentation but also inflammatory reactions due to overexposure of UVB.

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