[논문]The Beneficial Effect of Adenophorae Radix on DSS-induced Colitis in Mice

Jung, Ji-Wook; Oh, Sa-Rang; Ahn, Eun-Mi; Yang, Eun-Ju; Kim, Su-Jin

The Beneficial Effect of Adenophorae Radix on DSS-induced Colitis in Mice 원문보기

Journal of experimental & biomedical sciences = 대한의생명과학회지, v.19 no.3, 2013년, pp.188 - 194

Jung, Ji-Wook (Department of Herbal Medicinal Pharmacology, College of Herbal Bio-industry, Daegu Haany University) , Oh, Sa-Rang (College of Pharmacy, Keimyung University) , Ahn, Eun-Mi (Department of Herbal Foodceutical Science, College of Health and Welfare, Daegu Haany University) , Yang, Eun-Ju (Department of Clinical Laboratory Science, Daegu Haany University) , Kim, Su-Jin (Department of Cosmeceutical Science, Daegu Hanny University)

Abstract ▼ AI-Helper

Ulcerative colitis (UC) is an inflammatory bowel disease, which is a chronic gastrointestinal disorder. Adenophorae Radix (AR) has been used as a traditional medicine for various diseases including strengthening cardiac function, allaying a fever, and easing pain and cough. However, the regulatory effects of AR in intestinal inflammation are not yet understood. This study attempted to determine the effect of AR in dextran sulfate sodium (DSS) - induced colitis in mice. The colitis mice were induced by drinking water containing 5% DSS for 7 days. The results showed that mice treated with DSS showed remarkable clinical signs, including weight loss, and reduced colon length. Administration of AR attenuated weight loss, colon shortening and inhibited the levels of interleukin (IL) - 6 in DSS - treated colon tissues. These results provide experimental evidence that AR might be a useful therapeutic medicine for patients with UC.

주제어

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문제 정의

However, there has been no information on whether AR regulates intestinal inflammation. In this study, we were interested in determining whether AR has the effect in inflammatory diseases and chose the dextran sulfate sodium (DSS)‐induced mouse colitis model as the subject for this study. This model resembles human IBD, and is used for pharmacological analysis of potentially effective anti‐inflammatory agents (Copper et al.
Recently, traditional herbal medicine has been increased interest for the treatment of these disorders. This study investigated the effect of AR on DSS‐induced colitis. Treatment with AR reduced the weight loss and colon shortening caused by DSS.

제안 방법

The mice (n=6) were examined daily for weight loss, stool consistency, and the presence of gross bleeding. The mice were randomized into groups (n=6/ group) receiving AR (200 mg/kg), sulfasalazine (150 mg/kg) as a positive control, or water as a negative control. AR and sulfasalazine diluted with water (200 μl) were administered orally once a day from day 0 of DSS treatment.
To provide experimental evidence that AR might be a useful therapeutic drug for patients with UC, we examined the effects of AR on colitis induced by DSS. The specific aims were as follows: (I) to assay the effect of AR on clinical signs of colitis including weight loss, colon shortening, diarrhea, and occult/gross bleeding; (II) to investigate the effect of AR on IL‐6 levels in DSS‐treated colon tissues.

대상 데이터

Female BALB/c mice (six weeks old) were obtained from SamTaco animal facility (Gyeonggi, Korea). The mice were housed in a pathogen‐free environment for at least one week to allow them to adapt the environmental changes and were euthanized using CO2 inhalation at the end of the study.

데이터처리

of at least three experiments. The results were examined using an independent t‐ tests and ANOVA with a Tukey post hoc test. A P value < 0.

이론/모형

The supernatants were used as the cytokine containing extract. The levels of IL‐6 were measured using a modification of the enzyme‐linked immunosorbent assay (ELISA). Ninety‐six well plates were coated with 100 μlaliquots of anti‐mouse IL‐6 monoclonal Abs at 1.

성능/효과

In conclusion, we demonstrated at first that a treatment of AR could reduce significantly the clinical signs and the levels of inflammatory mediators in a colitis model caused by DSS treatment. Therefore, these results suggested that AR may be a useful therapeutic candidate for colitis.
Another common feature of the DSS‐induced model of colitis is an increase in DAI. Increased DAI score was remarkably inhibited in the group administered with AR compared to the group with DSS (Fig. 3).
In this study, we attempted to provide experimental evidence that AR might be a useful therapeutic drug for patients with UC. The finding of this study first demonstrated that AR inhibits inflammatory response and colon injury provoked by DSS treatment, and suggested an important effect by which AR attenuated intestinal inflammation.
The inhibitory effects of AR on the intestines of mice in DSS‐induced experimental colitis were evaluated. The physiological signs (weight loss, colon shortening, diarrhea, and occult/gross bleeding) induced by 5% DSS treatment for 7 days were observed, and their DAIs were calculated. Mice treated with DSS showed a significant weight loss compared to the control.

후속연구

Therefore, these results suggested that AR may be a useful therapeutic candidate for colitis. However, the further studies must be performed to elucidate the precise mechanism of AR for the treatment of intestinal inflammatory disorders.
, 2008). Therefore, further studies will be necessary in order to clarify more precisely the role of AR on the intestinal inflammation.

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