[논문]20S-dihydroprotopanaxadiol, a ginsenoside derivative, boosts innate immune responses of monocytes and macrophages

Kim, Mi-Yeon; Cho, Jae Youl

doi:10.5142/jgr.2013.37.293

20S-dihydroprotopanaxadiol, a ginsenoside derivative, boosts innate immune responses of monocytes and macrophages 원문보기

Journal of ginseng research = 高麗人參學會誌, v.37 no.3, 2013년, pp.293 - 299

Kim, Mi-Yeon (School of Systems Biological Science, Soongsil University) , Cho, Jae Youl (Department of Genetic Engineering, Sungkyunkwan University)

Abstract ▼ AI-Helper

20S-dihydroprotopanaxadiol (2H-PPD) is a derivative of protopanaxadiol, a glycone of ginsenosides prepared from Panax ginseng. Although ginsenosides and acidic polysaccharides are known to be major active ingredients in ginseng, the immunopharmacological activities of their metabolites and derivatives have not been fully explored. In this study, we aimed to elucidate the regulatory action of 2H-PPD on the function of monocytes and macrophages in innate immune responses. 2H-PPD was able to boost the phagocytic uptake of fluorescein isothiocyanate-dextran in macrophages and enhance the generation of radicals (reactive oxygen species) in sodium nitroprusside-treated RAW264.7 cells. The surface levels of the costimulatory molecules such as CD80 and CD86 were also increased during 2H-PPD treatment. In addition, this compound boosted U937 cell-cell aggregation induced by CD29 and CD43 antibodies, but not by cell-extracellular matrix (fibronectin) adhesion. Similarly, the surface levels of CD29 and CD43 were increased by 2H-PPD exposure. Therefore, our results strongly suggest that 2H-PPD has the pharmacological capability to upregulate the functional role of macrophages/monocytes in innate immunity.

주제어

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문제 정의

Because its source compound, PPD, is known to exhibit various biological activities, including anti-cancer, pro-apototic, and anti-inflammatory effects [15- 17], exploring the pharmacological actions of 2H-PPD could be beneficial for developing novel ginseng-derived compounds. Therefore, in this study, we investigated the immunomodulatory role of 2H-PPD on the innate immune responses of macrophages and monocytes.

제안 방법

Briefly, U937 cells were preincubated with 2H-PPD (0 to 40 µM) for 1 h at 37℃, and they were further incubated with function-activating (agonistic) anti-CD29 or anti-CD43 antibodies (1 μg/ mL each) in a 96-well plate. After a 30 min incubation, cell-cell clusters were identified by a homotypic cell-cell adhesion assay using a hemocytometer [4,5] and photographed with an inverted light microscope equipped with a COHU high-performance CCD (Diavert) video camera. For a cell-fibronection adhesion assay, U937 cells (5×10⁵ cells/well) were seeded on a fibronectin (50 mg/mL)-coated plate and incubated for 3 h [23].

대상 데이터

2H-PPD was obtained from Ambo Institute (Daejeon, Korea). The purity of the compound was greater than 98% according to HPLC analysis.
Louis, MO, USA). RAW264.7 and U937 cells were purchased from American Type Culture Collection (Manassas, VA, USA). Cell–cell-adhesion-inducing antibodies to CD43 and CD29 were used as reported previously [18,19].

데이터처리

Student’s t-test and one-way ANOVA were used to determine the statistical significance of the differences between values for the various experimental and control groups.

성능/효과

In conclusion, we found that 2H-PPD can boost the cellular functions of macrophages/monocytes such as phagocytic uptake, ROS generation, expression of costimulatory molecules, and cell-cell adhesion.

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