[논문]Distinct Oxidative Damage of Biomolecules by Arrays of Metals Mobilized from Different Types of Airborne Particulate Matters: SRM1648, Fine (PM2.5), and Coarse (PM10) Fractions

Park, Yong Jin; Lim, Leejin; Song, Heesang

doi:10.4491/eer.2013.18.3.139

Distinct Oxidative Damage of Biomolecules by Arrays of Metals Mobilized from Different Types of Airborne Particulate Matters: SRM1648, Fine (PM2.5), and Coarse (PM10) Fractions 원문보기

Environmental engineering research, v.18 no.3, 2013년, pp.139 - 143

Park, Yong Jin (Department of Emergency Medicine, Chosun University School of Medicine) , Lim, Leejin (Department of Biomaterials, Chosun University Graduate School) , Song, Heesang (Department of Biomaterials, Chosun University Graduate School)

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This study was performed to examine the in vitro toxicities which are incurred due to the mobilization metals from standard reference material (SRM) 1648, fine ($PM_{2.5}$), and coarse ($PM_{10}$) particulate matter collected in Seoul metropolitan area. DNA single strand breaks of approximately 74% and 62% for $PM_{2.5}$ and for $PM_{10}$, respectively, were observed in the presence of chelator (EDTA or citrate)/reductant (ascorbate), as compared to the control by 2% without chelator or reductant. $PM_{2.5}$ induced about 40% more carbonyl formation with proteins in the presence of EDTA/ascorbate than $PM_{10}$. Therefore, more damage to biomolecules was incurred upon exposure to $PM_{2.5}$ than to $PM_{10}$. The treatment of a specific chelator, desferrioxamine, to the reaction mixture containing chelator plus reductant decreased the extent of damage to DNA to the level of the control, but did not substantially decrease the extent of damage to proteins. This suggests that different arrays of metals were involved in the oxidation of DNA and proteins.

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Each experiment conducted in this study was repeated 3 times or more, and the results were reported as mean values ± standard deviation.
, San Leandro, CA, USA). The area obtained upon integration of the peaks for the representative forms of DNA (i.e., I and II) were then used to calculate the percentage of DNA with SSB in each lane. All results were reported as the percentage of SSB DNA ± standard deviation relative to the control DNA.
The goal of this study was to compare the metal-induced oxidative damages incurred by biomolecules through PM_2.5 exposure with those of PM₁₀, each of which was collected in Seoul metropolitan area. The oxidative damage caused by a standard reference material (SRM) 1648 was also investigated and compared with the results from PM_2.

성능/효과

All results were reported as the percentage of SSB DNA ± standard deviation relative to the control DNA.
In this study, the addition of a low molecular weight chelator (citrate or EDTA) greatly enhanced the reactivity of the particles with DNA and proteins (Tables 1–3).
The addition of PM2.5 and PM10 to the sample containing φX174 DNA resulted in the significant formation of DNA SSB of by 2% and 53%, respectively.
As has been observed with SRM1648, the incremental addition of PM, EDTA, and ascorbate incurred increasingly higher percentages of carbonylation to both of the proteins. The percentages of carbonylation in the presence of PM_2.5/EDTA/ascorbate were 494% and 776% for BSA and lysozyme, respectively, and those in the presence of PM₁₀/ EDTA/ascorbate were 339% and 580% for BSA and lysozyme, respectively. This result suggests that the presence of PM_2.
[17] reported that ultrafine particles, even if present in the atmosphere at very low mass concentrations, are involved in the observed pathologies because of their large numbers per unit volume and their extremely high surface/volume ratio. Therefore, it is expected that, considering both the present results and the previously known toxicities of the transition metals, PM_2.5is more damaging to various biomolecules than PM₁₀.

참고문헌 (17)

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Distinct Oxidative Damage of Biomolecules by Arrays of Metals Mobilized from Different Types of Airborne Particulate Matters: SRM1648, Fine (PM2.5), and Coarse (PM10) Fractions 원문보기

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Distinct Oxidative Damage of Biomolecules by Arrays of Metals Mobilized from Different Types of Airborne Particulate Matters: SRM1648, Fine (PM2.5), and Coarse (PM10) Fractions 원문보기

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