Objective: Low dose radiation may stimulate the growth and development of animals, increase life span, enhance fertility, and downgrade the incidence of tumor occurrence.The aim of this study was to investigate the antitumor effect and hormesis in an erythrocyte system induced by low-dose radiation....
Objective: Low dose radiation may stimulate the growth and development of animals, increase life span, enhance fertility, and downgrade the incidence of tumor occurrence.The aim of this study was to investigate the antitumor effect and hormesis in an erythrocyte system induced by low-dose radiation. Methods: Kunming strain male mice were subcutaneously implanted with S180 sarcoma cells in the right inguen as an experimental in situ animal model. Six hours before implantation, the mice were given 75mGy whole body X-ray radiation. Tumor growth was observed 5 days later, and the tumor volume was calculated every other day. Fifteen days later, all mice were killed to measure the tumor weight, and to observe necrotic areas and tumor-infiltration-lymphoreticular cells (TILs). At the same time, erythrocyte immune function and the level of 2,3-diphosphoglyceric acid (2,3-DPG) were determined. Immunohistochemical staining was used to detect the expression of EPO and VEGFR of tumor tissues. Results: The mice pre-exposed to low dose radiation had a lower tumor formation rate than those without low dose radiation (P < 0.05). The tumor growth slowed down significantly in mice pre-exposed to low dose radiation; the average tumor weight in mice pre-exposed to low dose radiation was lighter too (P < 0.05). The tumor necrosis areas were larger and TILs were more in the radiation group than those of the group without radiation. The erythrocyte immune function, the level of 2,3-DPG in the low dose radiation group were higher than those of the group without radiation (P < 0.05). After irradiation the expression of EPO of tumor tissues in LDR group decreased with time. LDR-24h, LDR-48h and LDR-72h groups were all statistically significantly different from sham-irradiation group. The expression of VEGFR also decreased, and LDR-24h group was the lowest (P < 0.05). Conclusion: Low dose radiation could markedly increase the anti-tumor ability of the organism and improve the erythrocyte immune function and the ability of carrying $O_2$. Low-dose total body irradiation, within a certain period of time, can decrease the expression of hypoxia factor EPO and VEGFR, which may improve the situation of tumor hypoxia and radiosensitivity of tumor itself.
Objective: Low dose radiation may stimulate the growth and development of animals, increase life span, enhance fertility, and downgrade the incidence of tumor occurrence.The aim of this study was to investigate the antitumor effect and hormesis in an erythrocyte system induced by low-dose radiation. Methods: Kunming strain male mice were subcutaneously implanted with S180 sarcoma cells in the right inguen as an experimental in situ animal model. Six hours before implantation, the mice were given 75mGy whole body X-ray radiation. Tumor growth was observed 5 days later, and the tumor volume was calculated every other day. Fifteen days later, all mice were killed to measure the tumor weight, and to observe necrotic areas and tumor-infiltration-lymphoreticular cells (TILs). At the same time, erythrocyte immune function and the level of 2,3-diphosphoglyceric acid (2,3-DPG) were determined. Immunohistochemical staining was used to detect the expression of EPO and VEGFR of tumor tissues. Results: The mice pre-exposed to low dose radiation had a lower tumor formation rate than those without low dose radiation (P < 0.05). The tumor growth slowed down significantly in mice pre-exposed to low dose radiation; the average tumor weight in mice pre-exposed to low dose radiation was lighter too (P < 0.05). The tumor necrosis areas were larger and TILs were more in the radiation group than those of the group without radiation. The erythrocyte immune function, the level of 2,3-DPG in the low dose radiation group were higher than those of the group without radiation (P < 0.05). After irradiation the expression of EPO of tumor tissues in LDR group decreased with time. LDR-24h, LDR-48h and LDR-72h groups were all statistically significantly different from sham-irradiation group. The expression of VEGFR also decreased, and LDR-24h group was the lowest (P < 0.05). Conclusion: Low dose radiation could markedly increase the anti-tumor ability of the organism and improve the erythrocyte immune function and the ability of carrying $O_2$. Low-dose total body irradiation, within a certain period of time, can decrease the expression of hypoxia factor EPO and VEGFR, which may improve the situation of tumor hypoxia and radiosensitivity of tumor itself.
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제안 방법
Randomly prepared each sample into 5 slices, each of which was observed 10 high-power fields (400X) under microscope to count the expression rates of EPO and VEGFR (expression rate=positive cells/all counting cell×100%).
is not reported. This study explored the effect of low dose radiation on tumor growth and erythrocyte immune function, the ability of carrying O2, and illuminates further the mechanism of hormesis of low dose radiation.
But there have been few reports about the effects of low dose radiation on erythrocyte immune function. This study explored the effects of low dose radiation on tumor growth and changes of erythrocyte immune function and the level of 2,3-diphosphoglyceric acid (2,3-DPG) in tumor-bearing mice. The study may provide theoretical evidence for the clinical use of low dose radiation.
대상 데이터
BeiJing F34-1 deep X-ray machine (BeiJing, China), voltage 200kv, electric current 10mA, filter 0.5mm Cu+1.0mmAl was used. The mice in the LDR group and pre-LDR group were packed in self-made paper box, and given whole body radiation, 75 mGy (total dosage), 12.
The materials of erythrocyte immune were provided by professor GuoFeng in Affilitated CangHai Hospital of the second military Medical University. 15 days after the implantation, blood was taken from the venous sinus of the right eye of the mice, anticoagulated by heparin, and then detected the erythyocyte immune function, the level of 2,3-DPG.
이론/모형
15 days after the implantation, blood was taken from the venous sinus of the right eye of the mice, anticoagulated by heparin, and then detected the erythyocyte immune function, the level of 2,3-DPG. Erythyocyte immune function was detected by the method of GuoFeng (Mauriello et al., 2013). The red blood cell’s surface’s C3b receptor rate (RBC-C3bRR) and red blood cell’s immune compound rate (RBC- IcR) were determined.
T-test was used to compare two groups. Student-Newman-Keuls test was used to analyze multi-group data. Numeration data was analyzed by X2-test.
성능/효과
All these indicated that low dose radiation could activate mice’s defensive system and improve anti-tumor ability, as a result, implanted tumor cells were inhibited and tumor growth was slowed down.
Analysis of EPO-positive index showed there were significant differences in statistics compared LDR-24h group with S group (p<0.05), LDR-48h and LDR-72h with S group (p<0.01), LDR-24h with LDR-48h (p<0.05, Table 2).
High expression of VEGFR could lead to curative effect of lowering and clinical treatment failure, and ultimately promote tumor development (Huang &Chen, 2008). The study found that after low-dose total body irradiation the expression of EGFR increased after a reduction. The reduction reached the minimum at 12h after irradiation, which is statistical significantly different from the S group (p<0.
This experiment found that after low-dose total body irradiation to mice bearing S180 sarcoma, the expressions of EPO and VEGF were decreased. This on the one hand restrained tumor growth, metastasis and recurrence, on the other hand when tumor cells were hypoxic the activity of HIF-1 increased, and also improved the expressions of EPO and VEGF.
Many experimental studies and clinical data showed that when malignant tumor was hypoxic erythropoietin (EPO) was in high expression, the hypoxic status could activate the vascular endothelial growth factor receptor (VEGFR) which promote the metastasis and recurrence of tumor, reduce the efficacy of radiotherapy and chemotherapy (Huang &Chen,2008). This study found that after low-dose total body irradiation on mice bearing S180 sarcoma, the EPO in tumor tissues was reduced, which indirectly reflect the hypoxic state in tumor tissues was improved.
후속연구
Tumor inhibition effects induced by low dose radiation are closely related to the improvement of erythrocyte immune function. But its mechanism is still not very clear, which needs further investigation.
This study explored the effects of low dose radiation on tumor growth and changes of erythrocyte immune function and the level of 2,3-diphosphoglyceric acid (2,3-DPG) in tumor-bearing mice. The study may provide theoretical evidence for the clinical use of low dose radiation.
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