Scutellaria baicalensis $G_{EORGI}$ (Scutellariae Radix) has been used to clear heat and to dry dampness in the stomach or intestines, which manifests as diarrhea or dysenteric disorder. In this study, we investigated the effective preparation of active components in Scutellariae Radix us...
Scutellaria baicalensis $G_{EORGI}$ (Scutellariae Radix) has been used to clear heat and to dry dampness in the stomach or intestines, which manifests as diarrhea or dysenteric disorder. In this study, we investigated the effective preparation of active components in Scutellariae Radix using the methods of solvent extraction and absorption fractionation for the development of new functional food or pharmaceuticals. The marker substances, baicalin, baicalein, wogonoside, and wogonin were directly isolated from the Scutellariae Radix. There chemical structures were elucidated by spectroscopic analysis. The Scutellariae Radix was extracted with hot water. To enhance yield of flavonoids in Scutellariae Radix, the hot water extract was dissolved in ethanol with concentration dependent manner. The precipitates were separated using centrifugal techniques at 10,000 rpm. Supernatant liquid was applied to the HPLC for quantification of major compounds. Separately, the hot water extract was absorbed on Diaion HP-20 resin. And then, the absorbed fraction was eluted with methanol for HPLC. The contents of baicalin, baicalein, wogonoside and wogonin in different treatment methods were analyzed by HPLC. Total amount of four major components were 16.9% in 50% ethanol extract, 21.7% in 70% ethanol extract, 20.5% in 90% ethanol extract, and 39.3% in absorbed fraction of Diaion HP-20 resin. In these results, we found that resin absorption method is suitable for the extraction of enriched flavonoids from Scutellariae Radix.
Scutellaria baicalensis $G_{EORGI}$ (Scutellariae Radix) has been used to clear heat and to dry dampness in the stomach or intestines, which manifests as diarrhea or dysenteric disorder. In this study, we investigated the effective preparation of active components in Scutellariae Radix using the methods of solvent extraction and absorption fractionation for the development of new functional food or pharmaceuticals. The marker substances, baicalin, baicalein, wogonoside, and wogonin were directly isolated from the Scutellariae Radix. There chemical structures were elucidated by spectroscopic analysis. The Scutellariae Radix was extracted with hot water. To enhance yield of flavonoids in Scutellariae Radix, the hot water extract was dissolved in ethanol with concentration dependent manner. The precipitates were separated using centrifugal techniques at 10,000 rpm. Supernatant liquid was applied to the HPLC for quantification of major compounds. Separately, the hot water extract was absorbed on Diaion HP-20 resin. And then, the absorbed fraction was eluted with methanol for HPLC. The contents of baicalin, baicalein, wogonoside and wogonin in different treatment methods were analyzed by HPLC. Total amount of four major components were 16.9% in 50% ethanol extract, 21.7% in 70% ethanol extract, 20.5% in 90% ethanol extract, and 39.3% in absorbed fraction of Diaion HP-20 resin. In these results, we found that resin absorption method is suitable for the extraction of enriched flavonoids from Scutellariae Radix.
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제안 방법
Chromatographic separation was performed on an YMC ODS a reversed-phase column (250 × 4.6 ㎜, 4 ㎛, YMC, Japan).
5 M phosphoric acid 1: 1) and were applied to the HPLC for quantification of major compounds such as baicalin, baicalein, wogonoside and wogonin. HPLC analysis was conducted using a Waters system (Waters Corp. Milford, MA, USA) consisting of binary-solvent delivery pump (Waters 1525), UV/Visible detector (Waters 2487). UV absorbance was monitored at 280 ㎚.
In this study, we found effective fraction method of high flavonoids contents in S. baicalensis for commercial application using absorption method by Diaion-HP 20 resin. Diaion HP-20 used in reverse phase chromatography is a non-polar polyaromatic adsorbent resin.
대상 데이터
The plant inhabits Sìchuan, Shandong in Chinese, east Russia, and entire region in Korea (Korea National Arboretum, 2014).
이론/모형
05). To analyze the amounts of the four active compounds in the solution, linear regression analysis for each compound was performed by the external standard method. All marker substances showed good linearity (r > 0.
성능/효과
, 2006). In conclusion, the present study showed that resin absorption method is useful as an extraction method for high flavonoid fractionation in S. baicalensis. Furthermore, we expect that this method could be applicable to the industrialization of functional food and herbal drug.
후속연구
Furthermore, we expect that this method could be applicable to the industrialization of functional food and herbal drug. In spite of these, further studies are required to compare with resin absorption, microwave extraction and ultrasonic-assisted extraction methods for the effective flavonoids fraction from natural sources.
참고문헌 (29)
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