[논문]Regulation of Nrf2 Mediated Phase II Enzymes by Luteolin in human Hepatocyte

Park, Chung Mu

Regulation of Nrf2 Mediated Phase II Enzymes by Luteolin in human Hepatocyte 원문보기

Biomedical science letters = 대한의생명과학회지, v.20 no.2, 2014년, pp.56 - 61

Park, Chung Mu (Department of Clinical Laboratory Science, Dong-Eui University)

Abstract ▼ AI-Helper

This study attempted to confirm the antioxidative potential of luteolin against tert-butyl hydroperoxide (t-BHP) induced oxidative damage and to investigate its molecular mechanism related to glutathione (GSH)-dependent enzymes in HepG2 cells. Treatment with luteolin resulted in attenuation of t-BHP induced generation of reactive oxygen species (ROS) and oxidative stress-mediated cell death. In addition, accelerated expression of GSH-dependent antioxidative enzymes, glutathione peroxidase (GPx) and glutathione reductase (GR), and heme oxygenase (HO)-1, as well as strengthened GSH content was induced by treatment with luteolin, which was in accordance with increased nuclear translocation of nuclear factor-erythroid 2 p45-related factor 2 (Nrf2), a transcription factor for phase 2 enzymes, in a dose-dependent manner. These results suggest that the cytoprotective potential of luteolin against oxidative damage can be attributed to fortified GSH-mediated antioxidative pathway and HO-1 expression through regulation of Nrf2 in HepG2 cells.

주제어

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가설 설정

The data are representative of three independent expreiments. (B) The relative induction of Nrf2 translocation was quantified by densitometry. PARP was used as an internal control.
The HepG2 cell line was obtained from the American Type Culture Collection (HB-8065; Rockville, MD, USA) and cultured in DMEM supplemented with 10% FBS and 2 mM L-glutamine. To investigate the question of whether luteolin can attenuate t-BHP induced oxidative damage, optimized conditions for oxidative damage-mediated cell death in HepG2 cells should be determined. Cells were treated with various concentrations of t-BHP for 0, 1, 3, 6, 12, and 24 hr.

제안 방법

The aim of this study was to analyze the protective activity of luteolin against oxidative damage and its underlying molecular mechanism related to nuclear factorerythroid 2 p45-related factor 2 (Nrf2)-mediated antioxidative cascade in HepG2 cells.
, 2006). Therefore, this study attempted to verify the protective activity of luteolin against t-BHP induced oxidative damage and to further investigate the molecular mechanism of the Nrf2-mediated GSH pathway and HO-1 expression in HepG2 cells.

대상 데이터

Luteolin (purity ≥98.0%), 2',7'-dichlorofluorescin diacetate (DCFH-DA), and dimethyl sulfoxide (DMSO) were purchased from Sigma (St. Louis, MO, USA).
The HepG2 cell line was obtained from the American Type Culture Collection (HB-8065; Rockville, MD, USA) and cultured in DMEM supplemented with 10% FBS and 2 mM L-glutamine. To investigate the question of whether luteolin can attenuate t-BHP induced oxidative damage, optimized conditions for oxidative damage-mediated cell death in HepG2 cells should be determined.

데이터처리

Significant differences between untreated control and luteolin treated groups were evaluated by unpaired Student's t-test.
Significant differences between untreated control and t-BHP treated groups were evaluated by unpaired Student's t-test.
The values were compared with the control using analysis of variance (ANOVA) followed by unpaired Student's t-test.

이론/모형

GSH was measured using an enzymatic recycling procedure described by Tietze (Tietze, 1969), in which GSH is sequentially oxidized by 5,5U-dithiobis (2-nitrobenzoic acid) and reduced by NADPH in the presence of GSH reductase.

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