선택한 단어 수는 입니다.
최소 단어 이상 선택하여야 합니다.
최소 단어 이상 선택하여야 합니다.
선택한 단어 수는 30입니다.
최대 10 단어까지만 선택 가능합니다.
최대 10 단어까지만 선택 가능합니다.
제안 방법
- In order to examine further the antioxidant properties of hwanggeumchal sorghum, glutinous sorghum, and barnyard millet grains, the 80% ethanol extracts of the three miscellaneous cereal grains were sequentially fractionated with n-hexane, methylene chloride, ethyl acetate and n-butanol, and then individual organic solvent fractions were tested for the DPPH and ABTS radical scavenging activities. As shown in Table 2, the ethyl acetate and butanol fractions exhibited more potent DPPH radical- and ABTS radical-scavenging activities as compared to other organic solvent fractions of hwanggeumchal sorghum, glutinous sorghum, and barnyard millet.
- Ferric thiocyanate (FTC) assay and thiobarbituric acid (TBA) assay, which are frequently used as antioxidant tests for plant and food components, are known to specifically monitor hydroperoxide and malonaldehyde (MA) as the products of lipid peroxidation, respectively. In order to examine whether the antioxidant properties of glutinous sorghum, hwanggeumchal sorghum and barnyard millet grains, which were detectable by DPPH radical- and ABTS radical-scavenging activities, could also prevent lipid peroxidation, we decided to investigate the effects of the 80% ethanol extracts, ethyl acetate fractions, and butanol fractions from hwanggeumchal sorghum, glutinous sorghum and barnyard millet grains on linoleic acid peroxidation by employing both FTC and TBA methods.
- The 80% ethanol extracts of hwanggeumchal sorghum, glutinous sorghum and barnyard millet grains, which appeared to possess more potent free radical-scavenging activities compared with those of other grains, were sequentially fractionated by n-hexane, methylene chloride, ethyl acetate and n-butanol. Since the DPPH radical- and ABTS radical-scavenging activities were mainly detected in the ethyl acetate fraction followed by the butanol fraction, the antioxidant activity of the ethyl acetate fractions have been examined further by investigating not only their inhibitory effect on lipid peroxidation using ferric thiocyanate (FTC) and thiobarbituric acid (TBA) methods, but also their cytoprotective effect on tertiary-butyl hydroperoxide (TBHP)-induced apoptotic cell death in human HL-60 cells.
- To evaluate antioxidant properties of miscellaneous cereal grains, which were harvested in Korea, including proso millet, yellow glutinous proso millet, hwanggeumchal sorghum, glutinous sorghum, white glutinous sorghum, yellow glutinous foxtail millet, non-glutinous foxtail millet, green glutinous foxtail millet, golden foxtail millet, barnyard millet and adlay, the free radical-scavenging activities of 80% ethanol extracts prepared from the individual grains were measured using DPPH and ABTS methods, both of which have widely been employed to evaluate free radical-scavenging activities of natural compounds in foods and biological systems [34, 43]. As shown in Table 1, the 80% ethanol extract of hwanggeumchal sorghum grains exhibited the highest DPPH radical-scavenging activity followed by those of glutinous sorghum and barnyard millet grains.
대상 데이터
- Dimethyl sulfoxide (DMSO), Folin-ciocaleau reagent, 1,1-diphenyl-2-picryl-hydrazl (DPPH), 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), tertiary-butyl hydroperoxide (TBHP), ferric thiocyanate (FTC), thiobarbituric acid (TBA), α-tocopherol, linoleic acid, ammonium thiocynate, thiobarbituric acid, and tannic acid were purchased from Sigma (St, Louis, MO, USA).
- Eleven miscellaneous cereal grains, including proso millet (polished grains), yellow glutinous proso millet (unpolished grains), hwanggeumchal sorghum (unpolished grains), glutinous sorghum (polished grains), white glutinous sorghum (unpolished grains), yellow glutinous foxtail millet (polished grains), non-glutinous foxtail millet (polished grains), green glutinous foxtail millet (polished grains), golden foxtail millet (unpolished grains), barnyard millet (unpolished grains), and adlay (polished grains), harvested in Korea were provided by National Institute of Crop Science, Rural Development Administration, Miryang, Gyeongnam 627-803, Korea. Individual dried grains were milled on a Blender 7012 (Dynamics Corporation, USA), and then extracted with 80% ethanol for 3 h at 80°C.
- The percent of inhibition of lipid peroxidation = 100–(A1–A0)×10, where A0 is the absorbance of the negative control and A1 is the absorbance of the extract samples [8]. The experiment was performed in triplicates.
데이터처리
- The statistical significance was calculated with Student’s t-test.
이론/모형
- Previously many studies have demonstrated a high correlation between the contents of total phenolic compounds in plants and their antioxidant capacity [7, 18, 33, 35, 47]. To investigate the correlation between antioxidant activities and total phenolic contents, the ethanol extracts and their organic solvent fractions were analyzed for total phenolic contents using Folin-Ciocalteau method.
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