손바닥선인장 보충이 고지방식이 흰쥐 골격근의 PPAR-γ 와 PGC-1α 단백질 발현 증가에 미치는 영향 Opuntia humifusa Supplementation Reduces Fat Weight by Increasing PPAR-γ and PGC-1α Protein Expression in the Skeletal Muscle of Rats원문보기
본 연구는 안정 시 고지방식이 흰쥐의 골격근에서 PPAR-${\delta}$, PPAR-${\gamma}$ 그리고 PGC-$1{\alpha}$ 단백질 발현에 손바닥선인장 보충이 미치는 효과에 대하여 연구하였다. SD계 수컷 흰쥐 16마리를 무작위로 대조군(CG, n=8)과 실험군(EG, n=8)으로 분류하였다. 8주 동안 대조군은 고지방식이를 부하하였으며, 실험군은 5% 손바닥선인장을 보충식이하였다. 본 실험결과, 복부지방과 고환부 지방 중량은 EG군이 CG군에 비해 유의하게 낮게 나타났다(p<0.01). 또한 혈당, 중성지방, 총콜레스테롤의 농도도 EG군이 CG군에 비해 유의하게 낮게 나타났다(p<0.01). 한편, 골격근에서 PPAR-${\gamma}$와 PGC-$1{\alpha}$ 단백질 발현은 EG군이 CG군에 비해 유의하게 높게 나타났다(p<0.05). 이상의 결과로부터 손바닥선인장 보충이 고지방식이 흰쥐의 혈당과 중성지방 농도의 감소와 골격근에서 PPAR-${\gamma}$와 PGC-$1{\alpha}$ 단백질 발현을 증가시킴으로서 체지방을 감소시켜 체중증가 억제에 긍정적인 영향을 미치는 것으로 나타났다.
본 연구는 안정 시 고지방식이 흰쥐의 골격근에서 PPAR-${\delta}$, PPAR-${\gamma}$ 그리고 PGC-$1{\alpha}$ 단백질 발현에 손바닥선인장 보충이 미치는 효과에 대하여 연구하였다. SD계 수컷 흰쥐 16마리를 무작위로 대조군(CG, n=8)과 실험군(EG, n=8)으로 분류하였다. 8주 동안 대조군은 고지방식이를 부하하였으며, 실험군은 5% 손바닥선인장을 보충식이하였다. 본 실험결과, 복부지방과 고환부 지방 중량은 EG군이 CG군에 비해 유의하게 낮게 나타났다(p<0.01). 또한 혈당, 중성지방, 총콜레스테롤의 농도도 EG군이 CG군에 비해 유의하게 낮게 나타났다(p<0.01). 한편, 골격근에서 PPAR-${\gamma}$와 PGC-$1{\alpha}$ 단백질 발현은 EG군이 CG군에 비해 유의하게 높게 나타났다(p<0.05). 이상의 결과로부터 손바닥선인장 보충이 고지방식이 흰쥐의 혈당과 중성지방 농도의 감소와 골격근에서 PPAR-${\gamma}$와 PGC-$1{\alpha}$ 단백질 발현을 증가시킴으로서 체지방을 감소시켜 체중증가 억제에 긍정적인 영향을 미치는 것으로 나타났다.
This study was conducted to investigate the effects of supplementation with Opuntia humifusa on the expression of peroxisome proliferator-activated receptor-delta (PPAR-${\delta}$), peroxisome proliferator-activated receptor-gamma (PPAR-${\gamma}$) and peroxisome proliferator-a...
This study was conducted to investigate the effects of supplementation with Opuntia humifusa on the expression of peroxisome proliferator-activated receptor-delta (PPAR-${\delta}$), peroxisome proliferator-activated receptor-gamma (PPAR-${\gamma}$) and peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-$1{\alpha}$) in the skeletal muscle of rats fed a high-fat diet. Sixteen Sprague-Dawley male rats at 6 weeks of age were randomly divided into 2 groups: a control diet group (CG, n=8) and an experimental diet group (EG, n=8). The rats were fed a high-fat diet (CG) or a high-fat diet supplemented with 5% O. humifusa (EG) for 8 weeks. The results showed that the abdominal fat pad and epididymal fat pad weights were significantly lower in the EG than in the CG (p<0.01). In the blood, serum glucose, triglycerides, and total cholesterol in the EG group were lower than in the CG (p<0.01). The expression of PPAR-${\gamma}$ and PGC-$1{\alpha}$ protein in the skeletal muscle of the EG was increased compared with that of the CG (p<0.05). These results indicate that 8 weeks of O. humifusa supplementation lowers serum glucose and triglyceride levels and suppresses weight gain by reducing fat weight through an increase in the expression of PPAR-${\gamma}$ and PGC-$1{\alpha}$ in the muscle tissue of rats.
This study was conducted to investigate the effects of supplementation with Opuntia humifusa on the expression of peroxisome proliferator-activated receptor-delta (PPAR-${\delta}$), peroxisome proliferator-activated receptor-gamma (PPAR-${\gamma}$) and peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-$1{\alpha}$) in the skeletal muscle of rats fed a high-fat diet. Sixteen Sprague-Dawley male rats at 6 weeks of age were randomly divided into 2 groups: a control diet group (CG, n=8) and an experimental diet group (EG, n=8). The rats were fed a high-fat diet (CG) or a high-fat diet supplemented with 5% O. humifusa (EG) for 8 weeks. The results showed that the abdominal fat pad and epididymal fat pad weights were significantly lower in the EG than in the CG (p<0.01). In the blood, serum glucose, triglycerides, and total cholesterol in the EG group were lower than in the CG (p<0.01). The expression of PPAR-${\gamma}$ and PGC-$1{\alpha}$ protein in the skeletal muscle of the EG was increased compared with that of the CG (p<0.05). These results indicate that 8 weeks of O. humifusa supplementation lowers serum glucose and triglyceride levels and suppresses weight gain by reducing fat weight through an increase in the expression of PPAR-${\gamma}$ and PGC-$1{\alpha}$ in the muscle tissue of rats.
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제안 방법
After the acclimatization period during week one, sixteen 6-week-old male Sprague-Dawley rats (Samtaco Bio Korea, Hwaseong, Korea) were randomly divided into two groups: a control diet group (CG: high-fat diet group, n=8) and an experimental diet group (EG: 5% O. humifusa supplemented diet group, n=8), given free access to tap water and food for 8 weeks, and housed in groups of two per cage under controlled temperature (23±1℃) and relative humidity (50±5%).
In order to determine the effect of O. humifusa supple-mentation on the body weight gain and fat tissue weights, both body weight and fat tissue weights were measured by using a digital electronic balance. As shown in Table 3, body weight gain was slightly lower in the EG compared with the CG after 8 weeks of O.
The enzymatic kits were used to estimate the effect of O. humifusa supplementation on serum parameters such as glucose, TG, HDLC, and TC. After the 8 weeks of the experiment, as shown in Table 4, the glucose, TG and TC concentrations of the EG were significantly lower than those of the CG (p<0.
This study investigated the effect of O. humifusa supplementation on body weight and changes in fat weight as wel as PPAR- δ, γ and PGC-1α protein expression on fatty acid metabolism in muscle tissue.
대상 데이터
O. humifusa, which was harvested in Asan, Chungnam, was cleaned and blended using a HMF-3150S blender (Hanil Electronics, Seoul, Korea). After blending, the O.
데이터처리
The data are expressed as the mean ± SE, and values were analyzed with the independent samples t test.
이론/모형
, Gyeonggi, Korea). After freeze-drying, as shown in Table 1, a general component analysis of O. humifusa was performed using the Association of Official Analytical Chemists (AOAC) method for the following measurements: moisture, using an air-oven method; ash, using a dry-ashing method; carbohydrates, using calculation; crude protein, using a Keldahl method; crude fat, using a Soxhlet extraction method; and fiber, using an enzymatic-chemical method. Mineral component analysis was performed using plasma atomic emission spectrometry (ICP-AES) to determine the composition of Fe2+, Ca2+, Mg2+, K+, Na+, and P2+.
성능/효과
After the 8 weeks of the experiment, as shown in Table 4, the glucose, TG and TC concentrations of the EG were significantly lower than those of the CG (p<0.01).
These results show that supplementation with O. humifusa for 8 weeks plays a positive role in lowering serum glucose and TG levels and suppressing weight gain by reducing fat weight through increasing the expression levels of PPAR-γ and PGC-1α proteins in the muscle tissue of rats.
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