기내배양 담배 식물에서 p-coumaric acid, benzoic acid 및 salicylic acid에 의해 유도되는 생장 및 glutathione reductase와 catalase의 활성 Effect of p-Coumaric Acid, Benzoic Acid, and Salicylic Acid on the Activity of Glutathione Reductase and Catalase in in vitro Grown Tobacco Plants원문보기
본 연구에서는 담배식물의 생장과 glutathione reductase와 catalase의 활성에 미치는 p-CA, BA 및 SA의 영향을 연구하였다. 담배식물의 생장에 미치는 p-CA, BA 및 SA의 영향을 알아보기 위하여, $10^{-5}$ mM p-CA, BA 및 SA가 각각 함유된 MS배지에서 배양한 후 20일부터 50일까지 10일 간격으로 담배의 생장을 비교하였다. 3가지 물질 모두에 의해 50일에서 생장이 가장 잘 되였다. Glutathione reductase의 활성에 미치는 효과를 측정한 결과, BA의 활성은 대조구보다 감소하였으며, p-CA 역시 활성이 감소하였고, SA의 활성은 증가하였다. 또한 모두 40일에서 가장 활성이 높았다. Catalase의 활성에 미치는 효과를 측정한 결과, SA의 활성은 증가하였으며, BA와 p-CA의 활성은 감소하였다. 또한 모든 실험구에서 40일에 활성이 가장 양호하였다. 결론적으로 p-CA와 BA는 담배식물의 생장을 촉진시켰으며, 50일에서 생장이 가장 양호하였으나 항산화 효소의 활성을 억제하였다. SA는 담배식물의 생장을 억제시켰으나 항산화 효소의 활성은 촉진시켰으며, 40일에서 가장 활성이 양호하였다.
본 연구에서는 담배식물의 생장과 glutathione reductase와 catalase의 활성에 미치는 p-CA, BA 및 SA의 영향을 연구하였다. 담배식물의 생장에 미치는 p-CA, BA 및 SA의 영향을 알아보기 위하여, $10^{-5}$ mM p-CA, BA 및 SA가 각각 함유된 MS배지에서 배양한 후 20일부터 50일까지 10일 간격으로 담배의 생장을 비교하였다. 3가지 물질 모두에 의해 50일에서 생장이 가장 잘 되였다. Glutathione reductase의 활성에 미치는 효과를 측정한 결과, BA의 활성은 대조구보다 감소하였으며, p-CA 역시 활성이 감소하였고, SA의 활성은 증가하였다. 또한 모두 40일에서 가장 활성이 높았다. Catalase의 활성에 미치는 효과를 측정한 결과, SA의 활성은 증가하였으며, BA와 p-CA의 활성은 감소하였다. 또한 모든 실험구에서 40일에 활성이 가장 양호하였다. 결론적으로 p-CA와 BA는 담배식물의 생장을 촉진시켰으며, 50일에서 생장이 가장 양호하였으나 항산화 효소의 활성을 억제하였다. SA는 담배식물의 생장을 억제시켰으나 항산화 효소의 활성은 촉진시켰으며, 40일에서 가장 활성이 양호하였다.
Effects of p-coumaric acid (p-CA), benzoic acid (BA), and salicylic acid (SA) on the activities of glutathione reductase and catalase were studied in in vitro grown tobacco plants. After culturing the tobacco plants in MS medium containing $10^{-5}$ mM of p-CA, BA, and SA, the increase in...
Effects of p-coumaric acid (p-CA), benzoic acid (BA), and salicylic acid (SA) on the activities of glutathione reductase and catalase were studied in in vitro grown tobacco plants. After culturing the tobacco plants in MS medium containing $10^{-5}$ mM of p-CA, BA, and SA, the increase in the activities of two enzymes, glutathione reductase and catalase, were compared from day 20 to day 50 day, with an interval of 10 days. The growth of the tobacco plants treated with p-CA, BA, and SA was the highest on day 50. Analysis of the effect of the three substances on the activity of glutathione reductase showed that BA and p-CA decreased the activity of the enzyme compared with a control, and SA increased the activity of the enzyme. All of them showed the highest activity on day 40. SA increased the activity of catalase, but BA and p-CA reduced the activity of this enzyme. In all the experimental groups, the activity was the highest on day 40. In conclusion, p-CA and BA appear to promote the growth of tobacco plants. The growth was the best on day 50, but the activity of the antioxidative enzyme was inhibited. On the contrary, SA seemed to inhibit the growth of the tobacco plants but to promote the activity of glutathione reductase and catalase. The growth of the plants treated with SA was best on day 40.
Effects of p-coumaric acid (p-CA), benzoic acid (BA), and salicylic acid (SA) on the activities of glutathione reductase and catalase were studied in in vitro grown tobacco plants. After culturing the tobacco plants in MS medium containing $10^{-5}$ mM of p-CA, BA, and SA, the increase in the activities of two enzymes, glutathione reductase and catalase, were compared from day 20 to day 50 day, with an interval of 10 days. The growth of the tobacco plants treated with p-CA, BA, and SA was the highest on day 50. Analysis of the effect of the three substances on the activity of glutathione reductase showed that BA and p-CA decreased the activity of the enzyme compared with a control, and SA increased the activity of the enzyme. All of them showed the highest activity on day 40. SA increased the activity of catalase, but BA and p-CA reduced the activity of this enzyme. In all the experimental groups, the activity was the highest on day 40. In conclusion, p-CA and BA appear to promote the growth of tobacco plants. The growth was the best on day 50, but the activity of the antioxidative enzyme was inhibited. On the contrary, SA seemed to inhibit the growth of the tobacco plants but to promote the activity of glutathione reductase and catalase. The growth of the plants treated with SA was best on day 40.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
제안 방법
In this study, effects of p-CA, BA and SA on the activity of glutathione reductase were studied. It was identified that p-CA and BA inhibited its activity and inhibition degree of BA was higher than that of p-CA.
Relating to anti-oxidation, this study identified effects of p-CA, BA and SA on the activity of catalase. It was reported that p-CA and BA inhibited the activity of catalase and inhibition degree of BA was stronger than that of p-CA.
이론/모형
The activity of glutathione reductase was determined by the method of Foyer and Halliwell [13]. The assay mixtures consisted of 25 mM Tris-HCl (pH 7.
성능/효과
It was suggested that it was increased gradually from 20 day to 40 day, the highest on 40 day, and reduced on 50 day. As results of comparing them with those of the control group, it was shown that all of their values from 20 day to 40 day were higher, but it was lower on 50 day.
As results of studying effects of p-CA, BA and SA on in vitro cultured tobacco plants, it was found that the growth from p-CA and BA was better than control group, but the growth from SA was lower than the control group. In addition, it was found that all of the three materials showed the lowest growth on 20 day, the increase of their growth until 50 day, and the highest growth on 50 day (Fig.
From the results on the effects of p-CA, BA and SA on the growth of tobacco plant, it was identified that while all the growth of BA group were increased from 20 day to 50 day, the growth of SA group was reduced and the growth of p-CA was increased from 30 day to 50 day compared with that of the control group. Therefore, it was identified that while p-CA and BA promoted the growth of tobacco plant and showed the best growth on 50 day, SA inhibited its growth.
In conclusion, it was identified that although p-CA and BA promoted the growth of tobacco plant, they inhibited activity of glutathione reductase and catalase. SA inhibited growth of tobacco plant but promoted activity of glutathione reductase and catalase.
There was a report that ozone increased the activity of glutathione reductase in tobacco plants [29]. It was considered that as p-CA and BA have lower activity of glutathione reductase than that of SA, the precursor and the analogue of SA have no effect in increasing the activity of glutathione reductase.
The values of catalase activity in the p-CA group with those of the control group were composed and it was found that all of them were lower than those of the control group from 20 day to 50 day. In the BA group, it was found that the activity was 0.
참고문헌 (42)
Aebi, H. 1984. Catalase in vitro. Methods Enzymol 105, 121-126.
Alscher, R. G. 1989. Biosynthesis and antioxidant function of glutathione in plants. Physiol Plant 77, 457-464.
An, S. M., Lee, S. I., Choi, S. W., Moon, S. W. and Boo, Y. C. 2008. p-Coumaric acid, a constituent of Sasa quelpaertensis Nakai, inhibits cellular melanogenesis stimulated by a-melanocyte stimulating hormone. J Dermatol 159, 292-299.
Asada, K. 1999. The water-water cycle in chloroplasts: scavenging of active oxygens and dissipation of excess photons. Ann Rev Plant Physiol Plant Mol Biol 50, 601-639.
Bi, Y. M., Kenton, P., Murr, L., Darby, R. and Draper, J. 1995. $H_2O_2$ does not function downstream of salicylic acid in the induction of PR protein expression. Plant J 8, 235-241.
Byun, H. J. and Choi, S. J. 2003. Activation of disease resistance related enzymes by treatment of hydrogen peroxide and benzoic acid in cucumber (Cucumis sativus). J Korean Soc Hort Sci 44, 287-291.
Chance, B., Sies, H. and Boveris, A. 1979. Hydroperoxide metabolism in mammalian organs. Physiol Rev 59, 527-605.
Creissen, G. P., Edwards, E. A. and Mullineaux, P. M. 1994. Glutathione reductase G. Pscorbate peroxidase, In Foyer, C. H. and Mullineaux, P. M. (eds.), pp. 343-364, Cause of Photooxidative stress and amelioration of defense systems in plants. CRC Press, Boca Raton, FL.
Durner, J. and Klessig, D. F. 1995. Inhibition of ascorbate peroxidase by salicylic acid and 2,6 dichloroisonicotinic acid, two inducers of plant defence responses. Proc Natl Acad Sci USA 92, 11312-11316.
Ferguson, L. R., Zhu, S. T. and Harris, P. J. 2005. Antioxidant and antigenotoxic effect of plant cell wall hydroxycinnamic acids in cultured HT-29 cell. Mol Nutr Food Res 49, 585-593.
Foyer, C. H. and Halliwell, B. 1976. the presence of glutathione and glutathione reductase in chloroplasts: A proposed role in ascorbic acid metabolism. Planta 133, 21-25.
Foyer, C. H., Lopez-Delgado, H., Dat, J. F. and Scott, I. M. 1997. Hydrogen peroxide and glutathione associated metabolism of acclimatory stress tolerance and signalling. Physiol Plant 100, 241-254.
Ganesan, V. and Thomas, G. 2001. Salicylic acid response in rice: influence of Salicylic acid on H2O2 accumulation and oxidative stress. Plant Science 160, 1095-1106.
Goodman, R. N. and Novacky, A. J. 1994. The hypersensitive reaction in plants to pathogens. APS Press., St. Paul MN USA.
Guan, L. and Scandalios, J. G. 1995. Developmentally related responses of maize catalase genes to salicylic acid. Proc Natl Acad Sci USA 92, 5930-5934.
Gutierrez-Coronado, M. A., Trejo-Lopez, C. and Larque-Saavedra, A. 1998. Effects of salicylic acid on the growth of roots and shoots in soybean. Plant Physiol Biochem 36, 563-565.
Hudson, E. A., Dinh, P. A., Kokubun, T., Simmonds, M. S. and Gescher, A. 2000. Characterization of potentially chemopreventive phenols in extracts of brown rice that inhibit the growth of human breast and colon cancer cells. Cancer Epidemiol Biomarkers Prev 9, 1163-1170.
Inze, D. and Van Montagu, M. 1995. Oxidative stress in plants. Curr Opin Biotech 6, 153-158.
Janda, T., Szalai, G., Tari, I. and Pa'ldi, E. 1999. Hydroponic treatment with salicylic acid decrease the effects of chilling injury in maize (Zea mays L.) plants. Planta 208, 175-180.
Leon, J., Yalpani, N., Raskin, I. and Lawton, M. A. 1993. Induction of benzoic acid 2-hydroxylase in virus-inoculated tobacco. Plant Physiol 103, 323-328.
Murphy, A. M., Chivasa, S., Singh, D. P. and Carr, J. P. 1999. Salicylic acid-induced resistance to viruses and other pathogens: A parting of the ways? Trends Plant Sci 4, 155-160.
Park, S. K. and Park, J. C. 1994. Antimicrobial activity of extracts and coumaric acid isolated from Artemisia princeps var. orientalis. Korean J Biotech Bioeng 5, 506-511.
Pasqualini, S., Batini, P., Ederli, L., Porceddu, A., Piccioni, C., De Marchis, F. and Antonielli, M. 2001. Effects of short-term ozone fumigation on tobacco plants: response of the scavenging system and expression of the glutathione reductase. Plant Cell Environ 24, 245-252.
Sanchez-Casas, P. and Klessig, D. F. 1994. A salicylic acidbinding activity and a salicylic acid-inhibitable catalase activity are present in a variety of plant species. Plant Physiol 106, 1675-1679.
Shakirova, F. M., Sakhabutdinova, A. R., Bezrukova, M. V., Fatkhutdinova, R. A. and Fatkhutdinova, D. R. 2003. Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant Science 164, 317-322.
Shin, D. H., Yu, S. R. and Choi, K. S. 1995. Effect of salicylic acid on anthocyanin synthesis in cell suspension cultures of Vitis vinifera L. Korean J Plant Tissue Culture 22, 59-64.
Smiri, M., Chaoui, A., Rouhier, N. and Gelhaye, E. 2010. Redox regulation of glutathione reductase/iso-glutaredoxin system in germinating pea seed exposed to cadmium. Plant Sci 179, 423-436.
Strobel, N. E. and Kuc, A. 1995. Chemical and biological inducers of systemic acquired resistance to pathogens protect cucumber and tobacco from damage caused by paraquat and cupric chloride. Phytopathol 85, 1306-1310.
Summermatter, K., Sticher, L. and Metraux, J. P. 1995. Systemic response in Arabidopsis thaliana infected and challenged with Pseudomonas syringae pv syringe. Plant Physiol 108, 1379-1385.
Tanaka, T., Naganuma, A. and Imura, N. 1990. Role of ${\gamma}$ -glutamyl-transpeptidase in renal uptake and toxicity in inorganic mercury in mice. Toxicology 60, 187-198.
Wang, D., Karolina, P. M., Angela, H. C. and Dong, X. 2007. Salicylic acid inhibits pathogen growth in plants through repression of the auxin signaling pathway. Curr Biol 17, 1784-1790.
Zang, L. Y., Cosma, G., Gardner, H., Shi, X., Castranova, V. and Vallyathan, V. 2000. Effect of antioxidant protection by p-coumaric acid on low-density lipoprotein cholesterol oxidation. Am J Physiol Cell Physiol 279, 954-960.
Zhao, F. Y., Wang, X. Y., Zhao, Y. X. and Zhang, H. 2006. Transferring the Suaeda salsa glutathione S-transferase and catalase genes enhances low temperature stress resistance in transgenic rice seedlings. J Plant Physiol Mol Biol 32, 231-238.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.