Lim, Sung Ha
(Department of Environmental Medical Biology, Yonsei University Wonju College of Medicine)
,
Koh, Yeong Kyeong
(Department of Environmental Medical Biology, Yonsei University Wonju College of Medicine)
,
Chang, Byung Soo
(Department of Cosmetology, Hanseo University)
,
Kim, Dong Heui
(Department of Environmental Medical Biology, Yonsei University Wonju College of Medicine)
The spermatogenesis of Siamese fighting fish, Betta splendens, belongs to Osphronemidae was investigated by light and electron microscopic observations. In primary spermatocyte stage, the nucleus was comparatively large ellipsoidal, and mitochondria showed a marked development in cytoplasm. In secon...
The spermatogenesis of Siamese fighting fish, Betta splendens, belongs to Osphronemidae was investigated by light and electron microscopic observations. In primary spermatocyte stage, the nucleus was comparatively large ellipsoidal, and mitochondria showed a marked development in cytoplasm. In secondary spermatocyte stage, the germ cells were smaller than that of primary spermatocytes. The nucleus was a spherical shape and intercellular space was formed between germ cells. In spermatid stage, the early spermatids were not much different from a secondary spermatocyte. But, the chromatin condensation was occurred from the outside to the inside. The nucleus was more condensed. Intracellular space was larger than early spermatid. The mitochondria were rearranged in a middle piece, and occupied about half of the head part in early sperm. In sperm stage, the head of mature sperm was a spherical shape and had no acrosome. The flagellum was showed the typical 9+2 array of microtubules. Also, the tail of sperm had no lateral fins and outer coarse fibers. These ultrastructural characteristics can be used in classification of species.
The spermatogenesis of Siamese fighting fish, Betta splendens, belongs to Osphronemidae was investigated by light and electron microscopic observations. In primary spermatocyte stage, the nucleus was comparatively large ellipsoidal, and mitochondria showed a marked development in cytoplasm. In secondary spermatocyte stage, the germ cells were smaller than that of primary spermatocytes. The nucleus was a spherical shape and intercellular space was formed between germ cells. In spermatid stage, the early spermatids were not much different from a secondary spermatocyte. But, the chromatin condensation was occurred from the outside to the inside. The nucleus was more condensed. Intracellular space was larger than early spermatid. The mitochondria were rearranged in a middle piece, and occupied about half of the head part in early sperm. In sperm stage, the head of mature sperm was a spherical shape and had no acrosome. The flagellum was showed the typical 9+2 array of microtubules. Also, the tail of sperm had no lateral fins and outer coarse fibers. These ultrastructural characteristics can be used in classification of species.
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제안 방법
Also, developmental stages are classified in a different way according to the researchers. In this study, the spermatogenesis of Betta splendens was classified into four stages, primary spermatocyte, secondary spermatocyte, spermatid, and sperm stages. But, Woodhead (1974) classified into six stages, primary spermatogonia, secondary spermatogonia, primary spermatocyte, secondary spermatocyte, spermatid, and spermatozoa under LM.
대상 데이터
A pair of deltoid testis of Siamese fighting fish, Betta splendens, with its white color, was located between air bladder and intestine. The size of testis was major axis 2.
4) for 2 hours at room temperature. Specimens were dehydrated in increasing concentrations of ethanol, cleared in propylene oxide, and embedded in an Epon mixture (PolyBed 812 kit; Polysciences Inc., USA). Ultrathin sections of Epon-embedded specimens were taken with an Ultracut E (Reichert-Jung, Austria) ultramicrotome at a thickness of about 60~70 nm.
The Siamese fighting fish, Betta splendens males used in this study were purchased from SanHo Aquarium (Wonju, Korea). They were separated and single fish were placed in 300 mL beaker within a glass aquarium (50×40×40 cm).
참고문헌 (30)
Alfert M (1956) Chemical differentiation of nuclear proteins during spermatogenesis in the salmon. J. Biophys. Biochem. Cytol. 2, 109-114.
Almeida F F, Taranger G L, Norberg B, Karlsen O, Bogerd J, and Schulz R W (2009) Photoperid-Modulated testis matureation in Atlantic cod (Gadus morhua, L.). Biol. Reprod. 80, 631-640.
Bailey M and Sandford G (1998) The New Guide to Aquarium Fish: A Comprehensive and Authoritative Guide to Tropical Freshwater, Brackish, and Marine Fishes. pp. 70-77, (Smithmark Publishers, New York).
Bayarri M J, Zanuy S, Yilmaz O, and Carrillo M (2009) Effects of continuous light on the reproductive system of European sea bass gauged by alterations of circadian variations during their first reproductive cycle. Chronobiol. 26, 184-199.
Billard R (1984) Ultrastructural changes in the spermatogonia and spermatocytes of Poecilia reticulata during spermatogenesis. Cell Tissue Res. 237, 219-226.
Chung E Y (2008) Ultrastructure of germ cells, the Leydig cells, and Sertoli cells during spermatogenesis in Boleophthalmus pectinirostris (Teleostei, Perciformes, Gobiidae). Tissue Cell 40, 195-205.
Deung Y K, Kim D H, and Reu D S (1999) Ultrastructure of gametes in the threespine stickleback, Gasterosteus aculeatus aculeatus. Korean J. Electron Microsc. 29, 177-178.
Doutrelant C, McGregor P K, and Oliveira R F (2001) The effect of an audience on intrasexual communication in male Siamese fighting fish, Betta splendens. Behav. Ecol. 12, 283-286.
Figler M H (1972) The relation between eliciting stimulus strength and habituation of the threat display in male Siamese fighting fish, Betta splendens. Behaviour 42, 63-96.
Fishelson L, Gon O, Holdengreber V, and Delarea Y (2007) Comparative spermatogenesis, spermatocytogenesis, and spermatozeugmata formation in males of viviparous species of clinid fishes (Teleostei: Clinidae, Blennioidei). Anat. Rec. 290, 311-323.
Gwo J C and Gwo H H (1993) Spermatogenesis in the black porgy, Acanthopagrus schlegeli (Teleostei: Perciformes: Sparidae). Mol. Reprod. Dev. 36, 75-83.
Joo K B and Kim D H (2013) Comparative ultrastructures of the fertilized egg envelopes in Danio rerio and Danio rerio var. frankei, Cyprinidae, Teleostei. Appl. Microsc. 43, 14-20.
Kim D H, Chang B S, Kim W J, Lee M S, Teng Y C, and Lee K J (2010) The Spermatogenesis of Chinese minnow, Leuciscinae, Teleostei. Korean J. Microsc. 40, 1-8.
Kim D H, Lee K J, Kim S, and Teng Y C (2009) The Spermatogenesis of Coreoleuciscus splendidus, Cyprinidae, Teleostei. Korean J. Microsc. 39, 227-236.
Kim D H, Reu D S, and Deung Y K (2003a) An ultrastructural study on the spermatogenesis of Xiphophorus maculatus. Korean J. Electron Microsc. 33, 267-274.
Kim J W, Kim B S, Choi C Y, and Lee J S (2003b) Ultrastructural study on the development of male germ cell of the olive flounder, Paralichthys olivaceus (Teleostei: Pleuronectidae). Korean J. Electron Microsc. 33, 243-250.
Kim S H, Lee C H, Song Y B, Hur S W, Kim H B, and Lee Y D (2013) Ultrastructure of late spermatids and spermatozoa during spermiogenesis in longtooth grouper Epinephelus bruneus from Jeju, Korea. Tissue Cell 45, 261-268.
Kirankumar S and Pandian T J (2002) Effect on growth and reproduction of hormone immersed and masculinized fighting fish Betta splendens. J. Exp. Zool. 293, 606-616.
Lee K J, Chang B S, Teng Y C, Kim S, Song M S, Joo K B, and Kim D H (2009) The Spermatogenesis of Cichlasoma managuensis, Cichlidae, Teleost. Korean J. Microsc. 39, 219-226.
Lobb M L and McCain G (1976) Proceedurally related differences in the aggressive behavior of Betta splendens (Regan). Animal Learning & Behavior 4, 367-373.
Mabee P M and Trendler T A (1996) Development of the cranium and paired fins in Betta splendens (Teleostei: Percomorpha): intraspecific variation and interspecific comparisons. J. Morphol. 227, 249-287.
Rebours C and Ottesen O H (2013) Ultrastructure of spermatozoa of the Atlantic cod Gadus morhua L. observed by scanning and transmission electron microscopy. Anim. Reprod. Sci. 139, 155-161.
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