Anaplasma species are obligate intracellular pathogens that can cause tick-borne diseases in mammalian hosts. To date, very few studies of their occurrence in Korean native goats (Capra aegagrus hircus) have been reported. In the present study, we investigated Anaplasma infection of Korean native go...
Anaplasma species are obligate intracellular pathogens that can cause tick-borne diseases in mammalian hosts. To date, very few studies of their occurrence in Korean native goats (Capra aegagrus hircus) have been reported. In the present study, we investigated Anaplasma infection of Korean native goats on Jeju Island, Republic of Korea, and performed phylogenetic analysis based on the 16S rRNA gene sequences. Our results showed that Anaplasma infection was found mostly in adult female goats. The phylogenetic tree revealed that the 7 sequences identified in Korean native goats could belong to Anaplasma sp. and were distinct from A. marginale, A. centrale, and A. ovis. The results indicated that the sequences identified to belong to Anaplasma were closely related to sequences isolated from goats in China and were clustered within the same group. To our knowledge, this is the first study to detect Anaplasma sp. infection in Korean native goats.
Anaplasma species are obligate intracellular pathogens that can cause tick-borne diseases in mammalian hosts. To date, very few studies of their occurrence in Korean native goats (Capra aegagrus hircus) have been reported. In the present study, we investigated Anaplasma infection of Korean native goats on Jeju Island, Republic of Korea, and performed phylogenetic analysis based on the 16S rRNA gene sequences. Our results showed that Anaplasma infection was found mostly in adult female goats. The phylogenetic tree revealed that the 7 sequences identified in Korean native goats could belong to Anaplasma sp. and were distinct from A. marginale, A. centrale, and A. ovis. The results indicated that the sequences identified to belong to Anaplasma were closely related to sequences isolated from goats in China and were clustered within the same group. To our knowledge, this is the first study to detect Anaplasma sp. infection in Korean native goats.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
문제 정의
Although there have been previous reports of Anaplasma infection in Korean native goats (Capra aegagrus hircus) [14,15], epidemiological studies on Anaplasma infection in these animals have not been well reported. Therefore, the objective of this study was to investigate Anaplasma infection in Korean native goats pastured on Jeju Island where ticks are most widely distributed and to perform molecular characterization of Anaplasma species detected on Jeju Island.
제안 방법
For the detection of Anaplasma infection, PCR was performed using the AccuPower® Rickettsiales 3-Plex PCR Kit (Bioneer, Daejeon, Korea).
데이터처리
Statistical analyses were performed by one-way ANOVA with IBM SPSS Statistics (version 19.0) and GraphPad Prism (version 6.0). In all statistical tests, P-value of <0.
이론/모형
Additional sequences from representative anaplasmosis isolates were obtained from the GenBank database and then integrated with each set of alignments. A phylogenetic tree based on the nucleotide alignment was constructed using the neighbor-joining method [17]. Bootstrap analysis was carried out with 1,000 replications and the tree was visualized using Treeview.
The 16S rRNA gene sequences identified in the present study are shown in the black-lined box. The unrooted phylogenetic tree was constructed using the neighbor-joining methods. Bootstrapping was carried out with 1,000 replications.
성능/효과
1). These isolates had a 98% similarity with A. centrale, 96.4-97.5% similarity with A. ovis, and 96.9-97.8% similarity with A. marginale. The phylogenetic analyses showed that the 7 Anaplasma sp.
참고문헌 (19)
1 Lew AE Gale KR Minchin CM Shkap V de Waal DT Phylogenetic analysis of the erythrocytic Anaplasma species based on 16S rDNA and GroEL (HSP60) sequences of A. marginale , A. centrale , and A. ovis and the specific detection of A. centrale vaccine strain Vet Microbiol 2003 92 145 160 12488078
2 Torina A Vicente J Alongi A Scimeca S Turlá R Nicosia S Di Marco V Caracappa S de la Fuente J Observed prevalence of tick-borne pathogens in domestic animals in Sicily, Italy during 2003-2005 Zoonoses Public Health 2007 54 8 15 17359441
3 Inokuma H Terada Y Kamio T Raoult D Brouqui P Analysis of the 16S rRNA gene sequence of Anaplasma centrale and its phylogenetic relatedness to other ehrlichiae Clin Diagn Lab Immunol 2001 8 241 244 11238202
4 Kocan KM de la Fuente J Blouin EF Garcia-Garcia JC Anaplasma marginale (Rickettsiales: Anaplasmataceae): recent advances in defining host-pathogen adaptations of a tick-borne rickettsia Parasitology 2004 129 285 300
5 Renneker S Abdo J Salih DE Karagenç T Bilgiç H Torina A Oliva AG Campos J Kullmann B Ahmed J Seitzer U Can Anaplasma ovis in small ruminants be neglected any longer? Transbound Emerg Dis 2013 60 105 112 24589109
6 Sreekumar C Anandan R Balasundaram S Rajavelu G Morphology and staining characteristics of Ehrlichia bovis Comp Immunol Microbiol Infect Dis 1996 19 79 83 8654048
8 Zhan L Cao WC Jiang JF Zhang XA Wu XM Zhang WY Liu W Zuo SQ Cao ZW Yang H Richardus JH Habbema JD Anaplasma phagocytophilum in livestock and small rodents Vet Microbiol 2010 144 405 408 20558015
9 Ramos RA Latrofa MS Giannelli A Lacasella V Campbell BE Dantas-Torres F Otranto D Detection of Anaplasma platys in dogs and Rhipicephalus sanguineus group ticks by a quantitative real-time PCR Vet Parasitol 2014 205 285 288 25027609
10 Geurden T Somers R Thanh NT Vien LV Nga VT Giang HH Dorny P Giao HK Vercruysse J Parasitic infections in dairy cattle around Hanoi, northern Vietnam Vet Parasitol 2008 153 384 388 18328629
11 Liu Z Ma M Wang Z Wang J Peng Y Li Y Guan G Luo J Yin H Molecular survey and genetic identification of Anaplasma species in goats from central and southern China Appl Environ Microbiol 2012 78 464 470 22057867
14 Baek BK Jin CM Seo SY Seo YW Kim DS Kakoma I A study on the epidemiology of caprine anaplasmosis in Korea Korean J Vet Res 1994 34 381 386
15 Lee SH Jung BY Kwak D Evidence of Anaplasma spp. exposure in native Korean goats ( Capra hircus coreanae ) Vet Med 2015 60 248 252
16 Thompson JD Gibson TJ Plewniak F Jeanmougin F Higgins DG The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools Nucleic Acids Res 1997 25 4876 4882 9396791
18 Kim CM Kim MS Park MS Park JH Chae JS Identification of Ehrlichia chaffeensis , Anaplasma phagocytophilum , and A. bovis in Haemaphysalis longicornis and Ixodes persulcatus ticks from Korea Vector Borne Zoonotic Dis 2003 3 17 26 12804377
19 Park JH Heo EJ Choi KS Dumler JS Chae JS Detection of antibodies to Anaplasma phagocytophilum and Ehrlichia chaffeensis antigens in sera of Korean patients by western immunoblotting and indirect immunofluorescence assays Clin Diagn Lab Immunol 2003 10 1059 1064 14607867
※ AI-Helper는 부적절한 답변을 할 수 있습니다.