Choi, Seung Kook
(Virology Unit, Department of Horticultural Environment, National Institute of Agricultural Biotechnology, Rural Development Agriculture)
,
Choi, Gug-Seoun
(Virology Unit, Department of Horticultural Environment, National Institute of Agricultural Biotechnology, Rural Development Agriculture)
,
Kwon, Sun-Jung
(Virology Unit, Department of Horticultural Environment, National Institute of Agricultural Biotechnology, Rural Development Agriculture)
,
Cho, In-Sook
(Virology Unit, Department of Horticultural Environment, National Institute of Agricultural Biotechnology, Rural Development Agriculture)
,
Yoon, Ju-Yeon
(Virology Unit, Department of Horticultural Environment, National Institute of Agricultural Biotechnology, Rural Development Agriculture)
Virus-like symptoms including stunt, severe mosaic with malformation of leaves, fern-like leaves and abnormal petals were observed from an African impatiens (Impatiens walleriana) grown in a plant nursery in Icheon, Korea. Serological analysis using immuno-strip kits for viruses reported in African ...
Virus-like symptoms including stunt, severe mosaic with malformation of leaves, fern-like leaves and abnormal petals were observed from an African impatiens (Impatiens walleriana) grown in a plant nursery in Icheon, Korea. Serological analysis using immuno-strip kits for viruses reported in African impatiens indicated that Cucumber mosaic virus (named CMV-Im) was a causal agent for the symptomatic African impatiens. Biological properties of CMV-Im were analyzed using responses of host plant species, suggesting that CMV-Im is a typical strain that belongs to CMV subgroup I. RT-PCR analysis verified CMV-Im infection from naturally infected African impatiens or mechanically inoculated some host species. Analysis of multiple alignments of CMV capsid protein (CP) sequences showed that CMV-Im shared high CP amino acids identities with other CMV strains. Phylogenetic tree analysis for the CP sequences of CMV-Im and representative CMV strains confirmed that CMV is a typical member of CMV subgroup I. To our knowledge, it is the first report of CMV in African impatiens in Korea.
Virus-like symptoms including stunt, severe mosaic with malformation of leaves, fern-like leaves and abnormal petals were observed from an African impatiens (Impatiens walleriana) grown in a plant nursery in Icheon, Korea. Serological analysis using immuno-strip kits for viruses reported in African impatiens indicated that Cucumber mosaic virus (named CMV-Im) was a causal agent for the symptomatic African impatiens. Biological properties of CMV-Im were analyzed using responses of host plant species, suggesting that CMV-Im is a typical strain that belongs to CMV subgroup I. RT-PCR analysis verified CMV-Im infection from naturally infected African impatiens or mechanically inoculated some host species. Analysis of multiple alignments of CMV capsid protein (CP) sequences showed that CMV-Im shared high CP amino acids identities with other CMV strains. Phylogenetic tree analysis for the CP sequences of CMV-Im and representative CMV strains confirmed that CMV is a typical member of CMV subgroup I. To our knowledge, it is the first report of CMV in African impatiens in Korea.
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제안 방법
(A) Immuno-strip assay for CMV diagnosis from naturally infected African impatiens or mechanically inoculated with CMV-Im. Sap extracts from systemic leaves of D. stramonium, N. benthamiana, and N. tabacum (cv. Xanthinc) inoculated with CMV-Im were analyzed using immune-strip kits for CMV. A sap extract from leaf tissues of healthy African impatiens were used as a negative control.
CMV was detected serologically from the naturally infected African impatiens but PMMoV, PepMoV, TMV, TBSV and TSWV were not detected from the African impatiens (data not shown). To further exclude the possibility of infection with Impatiens necrotic spot virus (INSV), the same positive controls and extract from healthy leaves of African impatiens were analyzed using a commercial immuno-strip for INSV (Agdia, USA), showing negative for INSV. These serological results suggest that CMV was a causal virus for the symptomatic African impatiens.
성능/효과
2% agarose gel and visualized after soaking in ethidium bromide (EtBr) solution. As expected, RTPCR products of approximately 660 bp were synthesized from the naturally infected African impatiens and a tobacco plant infected with CMV Fny strain (Fig. 3B), meanwhile none of RT-PCR product was amplified from a healthy African impatiens. These results confirm that a strain of CMV (named CMV-Im) was an authentic causal virus in the severe disease of African impatiens.
3B), meanwhile none of RT-PCR product was amplified from a healthy African impatiens. These results confirm that a strain of CMV (named CMV-Im) was an authentic causal virus in the severe disease of African impatiens. The amplified RT-PCR product of CMV-Im was directly cloned into the pGEM-T Easy vector (Promega, USA) to determine nucleotide sequence of the complete CP gene, according to the manufacturer’s instruction.
To verify further whether CMV was a major agent for the disease symptoms, sap extract of the symptomatic African impatiens was mechanically inoculated to indicator plant species, Chenopodium quinoa, Datura stramonium, Nicotiana benthamiana, N. tabacum (cv. Xanthi-nc), and African impatiens (I. walleriana). Local lesions on the inoculated leaves of C.
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