Content analysis of flavonoids (epicatechin, epicatechin gallate, and rutin) present in the leaves of Boehmeria nivea (originating from Geumsan-myeon, Biin-myeon, Hansan-myeon, and Baeksu-eup) and their commercial products (ramie tteok, ramie songpyeon, ramie bory-tteok, and ramie tea) was conducted...
Content analysis of flavonoids (epicatechin, epicatechin gallate, and rutin) present in the leaves of Boehmeria nivea (originating from Geumsan-myeon, Biin-myeon, Hansan-myeon, and Baeksu-eup) and their commercial products (ramie tteok, ramie songpyeon, ramie bory-tteok, and ramie tea) was conducted by HPLC. The content of epicatechin, epicatechin gallate, and rutin was highest in the leaves of B. nivea from Geumsan-myeon (0.138 mg/g), Baeksu-eup (1.654 mg/g) and Geumsan-myeon (12.205 mg/g), respectively. With respect to commercial products, the content of epicatechin and epicatechin gallate was highest in ramie tea, with concentrations of 1.879 and 1.090 mg/g, respectively. Given these flavonoid concentrations, B. nivea leaf extracts have the potential to be used as additives in natural medicinal products, health supplements, and beverages.
Content analysis of flavonoids (epicatechin, epicatechin gallate, and rutin) present in the leaves of Boehmeria nivea (originating from Geumsan-myeon, Biin-myeon, Hansan-myeon, and Baeksu-eup) and their commercial products (ramie tteok, ramie songpyeon, ramie bory-tteok, and ramie tea) was conducted by HPLC. The content of epicatechin, epicatechin gallate, and rutin was highest in the leaves of B. nivea from Geumsan-myeon (0.138 mg/g), Baeksu-eup (1.654 mg/g) and Geumsan-myeon (12.205 mg/g), respectively. With respect to commercial products, the content of epicatechin and epicatechin gallate was highest in ramie tea, with concentrations of 1.879 and 1.090 mg/g, respectively. Given these flavonoid concentrations, B. nivea leaf extracts have the potential to be used as additives in natural medicinal products, health supplements, and beverages.
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제안 방법
HPLC conditions- HPLC separation of 1, 2, and 3 for qualitative and quantitative analysis was performed using a reverse phase system. A Waters Spherisorb® ODS2 (4.
This study utilized HPLC to analyze the concentrations of epicatechin, epicatechin gallate, and rutin in the leaves of B. nivea originating from four different locations (Geumsan-myeon, Biin-myeon, Hansan-myeon, and Baeksueup), and in commercial products (ramie tteok, ramie songpyeon, ramie bory-tteok, and ramie tea).
The HPLC separation of analytes was conducted using a reverse phase system with a mobile phase consisting of water and MeOH (80 : 20 to 50 : 50 for 30 min) and all identified flavonoids were detected at 280 nm (Table 1). Using an optimized analytical method, compounds 1, 2, and 3 in the leaves of B. nivea were determined simultaneously (Fig. 2). The concentrations of 1, 2, and 3 were detected in B.
대상 데이터
(Pyeongtaek, Korea). Epicatechin (1), epicatechin gallate (2), and DMSO were purchased from Sigma-Aldrich (St. Louis, MO, USA). Electron ionizationmass spectrometry (EI-MS) was conducted with a Jeol JMS-600W mass spectrometer (Tokyo, Japan).
Sephadex LH-20 (20 - 100 μm) was purchase from Sigma-Aldrich. HPLC chromatograms of flavonoids were recorded with a Waters Breeze system (Massachusetts, USA) equipped with a Waters 1525 binary HPLC pump and a 2489 system UV/VIS detector. Water and MeOH used in this study were of HPLC grade, and all other reagents were of analytical grade.
nivea were cultivated and collected by Yeonggwang Agricultural Technology & Extension Center, Korea. The collection areas for B. nivea were Geumsan-myeon, Biin-myeon, Hansan-myeon, and Baeksu-eup, Korea.
참고문헌 (17)
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