Heo, Man Seung
(Interdisciplinary Program, Bioengineering Major, Graduate School, Seoul National University)
,
Moon, Hyun Seok
(Interdisciplinary Program, Bioengineering Major, Graduate School, Seoul National University)
,
Kim, Hee Chan
(Interdisciplinary Program, Bioengineering Major, Graduate School, Seoul National University)
,
Park, Hyung Woo
(Department of Neurosurgery, Seoul National University Hospital)
,
Lim, Young Hoon
(Department of Neurosurgery, Seoul National University Hospital)
,
Paek, Sun Ha
(Department of Neurosurgery, Seoul National University Hospital)
Objective : The purpose of this study to develop new deep-brain stimulation system for long-term use in animals, in order to develop a variety of neural prostheses. Methods : Our system has two distinguished features, which are the fully implanted system having wearable wireless power transfer and a...
Objective : The purpose of this study to develop new deep-brain stimulation system for long-term use in animals, in order to develop a variety of neural prostheses. Methods : Our system has two distinguished features, which are the fully implanted system having wearable wireless power transfer and ability to change the parameter of stimulus parameter. It is useful for obtaining a variety of data from a long-term experiment. Results : To validate our system, we performed pre-clinical test in Parkinson's disease-rat models for 4 weeks. Through the in vivo test, we observed the possibility of not only long-term implantation and stability, but also free movement of animals. We confirmed that the electrical stimulation neither caused any side effect nor damaged the electrodes. Conclusion : We proved possibility of our system to conduct the long-term pre-clinical test in variety of parameter, which is available for development of neural prostheses.
Objective : The purpose of this study to develop new deep-brain stimulation system for long-term use in animals, in order to develop a variety of neural prostheses. Methods : Our system has two distinguished features, which are the fully implanted system having wearable wireless power transfer and ability to change the parameter of stimulus parameter. It is useful for obtaining a variety of data from a long-term experiment. Results : To validate our system, we performed pre-clinical test in Parkinson's disease-rat models for 4 weeks. Through the in vivo test, we observed the possibility of not only long-term implantation and stability, but also free movement of animals. We confirmed that the electrical stimulation neither caused any side effect nor damaged the electrodes. Conclusion : We proved possibility of our system to conduct the long-term pre-clinical test in variety of parameter, which is available for development of neural prostheses.
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가설 설정
Implantation procedure. A : Wireless power transmission device. B : Implantable device.
A : Block diagram for asymmetric biphasic pulse stimulation. B : Formation of biphasic balanced stimulus waveform between cathode and anode channel. PWM : pulse-width modulation, SW : switch, LPF : low pass filter.
C : Microelectrode recording to find STN region. D : Fix the electrode by medical grade epoxy after implantation. E : Implant the device widthwise at lower dorsal area.
제안 방법
25 µL/min). 6) Apomorphine-induced rotation tests, which checks for loss of dopaminergic neuron, were performed from the first week on alternate weeks. Uniaxial rotational movement was observed after hypodermic injection to the back of the neck in PD-animals.
0 V, 130 Hz, 60 μs). After four weeks, the rotation tests were conducted on each model. As illustrated in Fig.
After perfusion, the rat brains were removed and placed, for one day, in a 50 mL conical tube containing ice-cold 4% paraformaldehyde. The rat brains were then transferred sequentially at one-day intervals into 10%, 20%, and 30% sucrose until they sank to the bottom of their container. The rat brains were then entirely cut into 30 μm-thick coronal sections on a freezing cryostat (Leica CM 3000, Leica, Solms, Germany).
The advantages of the proposed system are as followings : 1) long-term stability of the system, 2) free activity of the experimental animal, 3) the ability to change the amplitude of the stimulus, 4) information on implanted device status. To validate the system, we conducted experiments on PD-rodent models for 4 weeks. We confirmed that our system functions well and does not generate any harmful effect when used long-term.
대상 데이터
A 10×5 mm rectangular gold (99.9%; Sigma-Aldrich, Saint Louis, MO, USA) was used as a reference electrode.
For stimulating electrodes, a commercially available concentric stainless steel electrode (PlasticsOne, Roanoke, VA, USA) was used. The total length of the electrode was 17 mm, and the external tube had 15 mm and 2 mm opening.
Stimulator consisted of a Micro controller (MSP430F2013, TI), a stimulating waveform generator, and a data receiver (IR LED). The stimulating waveform was generated by the following process and Fig.
데이터처리
Results of the rotation test for each model. Rotation data were assumed to be non-normal distributed data, and they were compared using the Wilcoxon rank sum test. *p<0.
성능/효과
At the completion of the last behavior experiments, each rat was deeply anesthetized and perfused transcardially with about 50 mL of 0.9% NaCl (with heparine), followed by about 50 mL of ice-cold 4% paraformaldehyde.
The test was conducted while the distance between the transmission unit and the receiving unit was fixed as 2 mm. The results of the test showed that the system was operable for 24 h based on a 500 mAh lithium-polymer battery.
참고문헌 (14)
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