[논문]Comparing the mortality of Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae) caused by entomopathogenic bacteria and Serratia marcescens (Enterobacteriales: Enterobacteriaceae)

Kwak, Kyu Won; Han, Myung Sae; Nam, Sung Hee; Choi, Ji Young; Lee, Seok Hyun; Kim, Hong Geun; Park, Kwan Ho

doi:10.7852/ijie.2015.30.2.40

Comparing the mortality of Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae) caused by entomopathogenic bacteria and Serratia marcescens (Enterobacteriales: Enterobacteriaceae) 원문보기

International journal of industrial entomology, v.30 no.2, 2015년, pp.40 - 44

Kwak, Kyu Won (Department of Agricultural Biology, National Academy of Agricultural Science) , Han, Myung Sae (Department of Biofibers and Material Science, Kyungpook National University) , Nam, Sung Hee (Department of Agricultural Biology, National Academy of Agricultural Science) , Choi, Ji Young (Department of Agricultural Biology, National Academy of Agricultural Science) , Lee, Seok Hyun (Department of Agricultural Biology, National Academy of Agricultural Science) , Kim, Hong Geun (Department of Agricultural Biology, National Academy of Agricultural Science) , Park, Kwan Ho (Department of Agricultural Biology, National Academy of Agricultural Science)

Abstract ▼ AI-Helper

To investigate whether Serratia marcescens (Enterobacteriales: Enterobacteriaceae) isolated from Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae) acts as an opportunistic bacterium in peroral infection, the primary entomopathogenic bacteria Bacillus thuringiensis (Bacillales: Bacillaceae) and Paenibacillus popilliae (Eubacteriales: Bacillaceae) were added to sawdust to perform a bioassay experiment. We found that peroral infection caused by S. marcescens could be fatal beyond a concentration of $4{\times}10^8pfu/mL$ in $2^{nd}$ stage P. b. seulensis larvae and at $6{\times}10^8pfu/mL$ in $3^{rd}$ stage P. b. seulensis larvae. In particular, mortality resulting from a combination of P. popilliae and S. marcescens was markedly increased in $2^{nd}$ stage P. b. seulensis larvae. Therefore, we confirmed that mortality was increased when S. marcescens was infected together with other entomopathogenic bacteria, and that peroral infection itself can be fatal beyond certain concentrations.

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대상 데이터

Bacteria

Bacillus thuringiensis (KACC10169) and Serratia marcescens (KACC11892) were purchased from the Korean Agricultural Culture Collection (KACC, Wanju, Korea). Paenibacillus popilliae (KCTC3806) was purchased from the Korean Collection for Type Cultures (KCTC, Daejeon, Korea).
Bacillus thuringiensis (KACC10169) and Serratia marcescens (KACC11892) were purchased from the Korean Agricultural Culture Collection (KACC, Wanju, Korea). Paenibacillus popilliae (KCTC3806) was purchased from the Korean Collection for Type Cultures (KCTC, Daejeon, Korea). Lyophilized bacteria were incubated on nutrient agar plates, grown for 18–24 h, and colonies were isolated.

성능/효과

2015). Accordingly, increased monitoring has been initiated for diseases occurring in farms of Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae), an edible insect that is mass-reared in Korea, as well as the Japanese rhinoceros beetle Allomyrina dichotoma (Coleoptera: Scarabaeidae), and diseases with a high incidence were identified. Insects are mainly infected by fungi, bacteria, viruses, and protozoa, and mostly show cross-infection of fungi-bacteria, fungi-viruses, or fungi-bacteria-viruses.
marcescens are detected together in a breeding environment or in sawdust, the results of this study can be utilized in the prevention and diagnosis of such cases. S. marcescens, an entomopathogen, has been reported to be an opportunistic bacterium (Murdoch et al. 2011), but the results of this study confirmed that it can also show higher mortality than B. thuringiensis and P. popilliae, beyond a certain concentration, which proves that its fatal effects are stronger at younger larval stages. Furthermore, this study provides valuable information with respect to predicting the effect of these bacteria on mortality when they are detected together at insect breeding sites, which will make prevention possible.
seulensis breeding farms and is known as an opportunistic bacterium. The results of this study confirmed that mortality increased in insects with peroral infections of S. marcescens, similar to other bacteria. We observed mortality after 4 wk when insect sawdust was treated with Sm together with Bt and Pp, which are known to be pathogenic in Coleoptera.

후속연구

popilliae, beyond a certain concentration, which proves that its fatal effects are stronger at younger larval stages. Furthermore, this study provides valuable information with respect to predicting the effect of these bacteria on mortality when they are detected together at insect breeding sites, which will make prevention possible. In future studies, it will be necessary to investigate the mechanisms of how S.
Furthermore, this study provides valuable information with respect to predicting the effect of these bacteria on mortality when they are detected together at insect breeding sites, which will make prevention possible. In future studies, it will be necessary to investigate the mechanisms of how S. marcescens interacts with P. popilliae to maximize mortality and to examine the specific mortality rate of each bacterium for each stage of insect development.
85% higher than the Pp treatment alone. Since mortality is maximal when P. popilliae and S. marcescens are detected together in a breeding environment or in sawdust, the results of this study can be utilized in the prevention and diagnosis of such cases. S.

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