Hong, Se Chul
(International Ginseng & Herb Research Institute)
,
Yoo, Ji Hyun
(International Ginseng & Herb Research Institute)
,
Oh, Myeong Hwan
(International Ginseng & Herb Research Institute)
,
Lee, Hwan
(International Ginseng & Herb Research Institute)
,
Park, Young Sik
(International Ginseng & Herb Research Institute)
,
Parthasarathi, Shanmugam
(International Ginseng & Herb Research Institute)
,
Park, Jong Dae
(International Ginseng & Herb Research Institute)
,
Pyo, Mi Kyung
(International Ginseng & Herb Research Institute)
Pueraiae Radix (PR), Pueratia Folium (PF) and Sorbus commixta (SC) mixture, namely GS-SP (PR (1)/PF (2)/SC (0.5): v/v/v) was developed as hangover-relieving elixir and its effects on alcoholic metabolism have been investigated. The enzymatic activity of alcohol dehydrogenase (ADH) and acetaldehyde d...
Pueraiae Radix (PR), Pueratia Folium (PF) and Sorbus commixta (SC) mixture, namely GS-SP (PR (1)/PF (2)/SC (0.5): v/v/v) was developed as hangover-relieving elixir and its effects on alcoholic metabolism have been investigated. The enzymatic activity of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) of GS-SP was shown higher than those of single treatment with PR, PL, SC, and the positive control group (YM-808). The survival rate of mouse liver cell line NCTC clone 1469 in the presence of acetaldehyde was 30.6, 22.2, and 8.7% at the GS-SP dosage level of 50, 100, and 200 μg/mL respectively. Different concentrations of 50, 100 and 200 mg/kg of GS-SP showed efficient activity for ADH and ALDH than YM-808 in rat fed with 25% ethanol. The levels of blood alcohol and acetaldehyde after oral administration of 200 mg/kg of GS-SP showed efficient activity of 11.7% and 37% than those of YM-808. These results have been supported to the potential for GS-SP to serve as an excellent potential in providing hangover relief and liver protection.
Pueraiae Radix (PR), Pueratia Folium (PF) and Sorbus commixta (SC) mixture, namely GS-SP (PR (1)/PF (2)/SC (0.5): v/v/v) was developed as hangover-relieving elixir and its effects on alcoholic metabolism have been investigated. The enzymatic activity of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) of GS-SP was shown higher than those of single treatment with PR, PL, SC, and the positive control group (YM-808). The survival rate of mouse liver cell line NCTC clone 1469 in the presence of acetaldehyde was 30.6, 22.2, and 8.7% at the GS-SP dosage level of 50, 100, and 200 μg/mL respectively. Different concentrations of 50, 100 and 200 mg/kg of GS-SP showed efficient activity for ADH and ALDH than YM-808 in rat fed with 25% ethanol. The levels of blood alcohol and acetaldehyde after oral administration of 200 mg/kg of GS-SP showed efficient activity of 11.7% and 37% than those of YM-808. These results have been supported to the potential for GS-SP to serve as an excellent potential in providing hangover relief and liver protection.
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제안 방법
8) in place of ADH. ADH activity was estimated by comparing the measured maximum absorbance upon completion of the reaction with the absorbance of the control group and calculating the relative percentage activity.
To determine the effects of GS-SP extract on hangovers, experiments for measuring the blood alcohol and acetaldehyde levels were measured at time lapsed condition after alcohol consumption. Animal experiment was conducted with distilled water administered to the control group and GS- SP mixture to the test group at 50, 100, 200㎎/㎏ of body weight. Positive control group used the commercially available hangover relief beverage (YM-808).
Animal treatments−Sprague-Dawley (SD) male rats 4-week-old, 90±10g weight were used in this experiment and were well adapted to the laboratory conditions for 1 week before starting the experimentation. To determine the effects of GS-SP extract on hangovers, experiments for measuring the blood alcohol and acetaldehyde levels were measured at time lapsed condition after alcohol consumption.
Positive control group used the commercially available hangover relief beverage (YM-808). Each group had a single oral administration of the sample, and after 1h, 25% ethanol (15㎎/㎏ of body weight) was administered. The blood levels of ADH, ALDH, acetal- dehyde, and alcohol were measured after 2, 4, and 8h, respectively.
In this study, we determined ideal mixture proportioning using PF, PR, and SC, which are known to be excellent in preventing liver damage and oxidative stress4, 7 and investigated its biological activities to develop functional food materials for hangover relief and liver protection.
대상 데이터
, Korea). Alcohol dehydrogenase (Sigma A7011- 30KU), Aldehyde dehydrogenase (Sigma 82884) and Nicotinamide adenine dinucleotide (NAD+) (Sigma 43407) were purchased from Sigma (Sigma chemical. co., USA).
(Geumsan, Korea). The samples were deposited in the International Ginseng and Herb Research Institute [(No.; GS201203 (PR)], GS201204 (PF), GS201205 (SC)). Yeo-Myung 808 (YM-808) was purchased from Glami (Glami co.
; GS201203 (PR)], GS201204 (PF), GS201205 (SC)). Yeo-Myung 808 (YM-808) was purchased from Glami (Glami co., Korea). Alcohol dehydrogenase (Sigma A7011- 30KU), Aldehyde dehydrogenase (Sigma 82884) and Nicotinamide adenine dinucleotide (NAD+) (Sigma 43407) were purchased from Sigma (Sigma chemical.
데이터처리
Values are mean±SE (n=3). Same letters (a-c) are not significantly different at p<0.05 by Tukey's multiple range test after one-way analysis of variance (ANOVA).
Statistical analysis−The results was expressed as mean±S.E. Statistical analysis was done using one-way analysis of variance (ANOVA) followed by Tukey's multiple range test (SPSS VER. 21; SPSS Inc., Chicago, IL, USA). Statistical significance was considered at p< 0.
이론/모형
Alcohol dehydrogenase (ADH) enzyme activity− ADH activity was measured by following Reacker’s method8, using spectrophotometer set at 340nm to measure the absorption of NADH. Thus, a total of 1.
성능/효과
4% with the positive control group YM-808. Analysis of ALDH activity showed 135.5, 133.5, 128.6, and 126.2% in GS- SP, PR, SC and PR, respectively, with an activity of 133.4% with the positive control YM-808. ADH and ALDH activity increased 5.
In conclusion to this study, GS-SP was shown to increase enzymatic activity of ADH and ALDH, ultimately reducing blood alcohol and acetaldehyde levels in rat. This in turn consequently leads to hangover relief and prevention of alcoholic liver- damage.
참고문헌 (18)
Lieber, C. S. Gastroenterology. 1994, 106, 1085-1105.
Lieber, C. S. N. Engl. J. Med. 1973, 288, 356-362.
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