Mimosa pudica (미모사), Argemone mexicana (멕시코 가시양귀비) Leucus aspara (꿀풀과 일종), Polygonum hydropiper (여뀌), Blumea lacera (국화과 일종) 등 5종의 식물 헥산추출물들의 팥바구미 성충에 대한 살충, 성충우화억제력 및 녹두 종실피해 방제력이 검증되었다. 그 결과, 밭바구미 성충 살충력은 35-69%이었으며, 녹두에 추출물을 처리한 후 성충우화 방제율은 33-63%, 종실 피해 방제율은 13-49%이었다. 추출물의 농도가 증가할수록 살충력, 성충우화 및 종실피해 방제율 등이 증가하는 것으로 나타났다. 이와 같이 5종의 식물 헥산추출물들은 밭바구미 친환경 방제제로 이용가능성이 있을 것으로 판단된다.
Mimosa pudica (미모사), Argemone mexicana (멕시코 가시양귀비) Leucus aspara (꿀풀과 일종), Polygonum hydropiper (여뀌), Blumea lacera (국화과 일종) 등 5종의 식물 헥산추출물들의 팥바구미 성충에 대한 살충, 성충우화억제력 및 녹두 종실피해 방제력이 검증되었다. 그 결과, 밭바구미 성충 살충력은 35-69%이었으며, 녹두에 추출물을 처리한 후 성충우화 방제율은 33-63%, 종실 피해 방제율은 13-49%이었다. 추출물의 농도가 증가할수록 살충력, 성충우화 및 종실피해 방제율 등이 증가하는 것으로 나타났다. 이와 같이 5종의 식물 헥산추출물들은 밭바구미 친환경 방제제로 이용가능성이 있을 것으로 판단된다.
This study was conducted with n-hexane extracts of sensitive plant Mimosa pudica, mexican poppy Argemone mexicana, panimarich Leucus aspara, water pepper Polygonum hydropiper and shialmutra Blumea lacera weeds against pulse beetle Callosobruchus chinensis (Coleoptera: Bruchidae) for protection of mu...
This study was conducted with n-hexane extracts of sensitive plant Mimosa pudica, mexican poppy Argemone mexicana, panimarich Leucus aspara, water pepper Polygonum hydropiper and shialmutra Blumea lacera weeds against pulse beetle Callosobruchus chinensis (Coleoptera: Bruchidae) for protection of mung bean Vigna radiata grains. The $LC_{50}$ values of the weed extracts ranged from 4.5 to 6.4, 4.1 to 5.6 and 3.6 to 5.5 g/100 mL at 24, 48 and 72 hours of post treatment, respectively. The extracts showed 35 to 69% fecundity and 33 to 63% adult emergence inhibitory effect on the pest, and revealed 13 to 49% grain protection of mung beans. Insect mortality, fecundity and adult emergence inhibitory effects, and grain protection activity increased with increased concentration of the extracts. The shialmutra followed by water pepper extracts revealed better performances in fecundity and adult beetle emergence inhibitory effect compared to the other weeds. The findings proved that the n-hexane extracts of the five weeds are sources of botanical insecticides which may be used in the integrated management of C. chinensis.
This study was conducted with n-hexane extracts of sensitive plant Mimosa pudica, mexican poppy Argemone mexicana, panimarich Leucus aspara, water pepper Polygonum hydropiper and shialmutra Blumea lacera weeds against pulse beetle Callosobruchus chinensis (Coleoptera: Bruchidae) for protection of mung bean Vigna radiata grains. The $LC_{50}$ values of the weed extracts ranged from 4.5 to 6.4, 4.1 to 5.6 and 3.6 to 5.5 g/100 mL at 24, 48 and 72 hours of post treatment, respectively. The extracts showed 35 to 69% fecundity and 33 to 63% adult emergence inhibitory effect on the pest, and revealed 13 to 49% grain protection of mung beans. Insect mortality, fecundity and adult emergence inhibitory effects, and grain protection activity increased with increased concentration of the extracts. The shialmutra followed by water pepper extracts revealed better performances in fecundity and adult beetle emergence inhibitory effect compared to the other weeds. The findings proved that the n-hexane extracts of the five weeds are sources of botanical insecticides which may be used in the integrated management of C. chinensis.
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제안 방법
The beetles were removed after one week and the Petri dishes along with pulse grains were kept in the laboratory. After one month (bruchids start to emerge 30 days after eggs are laid) daily observation was started in each Petri dish and continued up to 15 days to record and remove the newly emerged beetles. The adult emergence inhibitory rate (%IR) was calculated by using the formula-Total number of damaged grains in each Petri dish was counted at the end of the experiment and percent grain damage inhibition (%DI) was calculated by the following formula-
chinensis management with the rationalize use of botanical pesticides, especially with the extracts of indigenous weeds. The purpose of this study was to evaluate the n-hexane extracts of sensitive plant Mimosa pudica, mexican prickly poppy Argemone mexicana, panimarich Leucus aspara, water pepper Polygonum hydropiper and shialmutra Blumea lacera weeds on the mortality, fecundity and adult emergence of C. chinensis reared on mung bean grains. Effect of the extracts on grain damage inhibition was also examined.
대상 데이터
Fifty pairs of unsexed C. chinensis beetles were obtained from the Entomology Laboratory stock of Hajee Mohammad Danesh Science and Technology University (HSTU), Dinajpur, Bangladesh. The beetles were introduced into 1 L glass jar containing 500 g of mung bean grains.
In each 250 mL glass jar, 100 g mung bean grains were put and the extract doses were distinctly added to the grains with pipette and subjected to manual agitation for 2 minutes. Five pairs of 0-72 h old C. chinensis beetles were packed in each glass jar with perforated lid, coated with thin fabric (voile) to allow gas exchange. After 7 days, the number of eggs laid (fecundity per five females) by the females in the jars was counted by using hand lens.
Ten adult beetles (2-3 days old) were placed at the center of a piece of filter paper and the paper was twisted to enclose them. The papers along with the insects were then dipped separately in 1, 2 and 2% extract concentrations or n-hexane for 30 seconds.
성능/효과
Our study proved that the weed extracts prevented fecundity and adult emergence of C. chinensis and the activity of the extracts increased with increased doses. The bioactive compounds in the weed caused death of the beetles and seemed to be inhibited egg hatching, larval development to the pupal and adults.
Among the treatments, 4% shialmutra revealed most toxicity effect at 72 h of post treatment. The findings of this study indicated that the weed species acted as valuable sources of insecticides, which proved for the rationalize use of botanical pesticides against stored grain pests. The ingredients of these weeds may have the ability to inject into the body of the beetles and dysfunction their nutritional valance, and eventually to death.
The tested weed extracts showed significant effects on C. chinensis and their toxicity were dose and exposure duration dependent. Among the treatments, 4% shialmutra revealed most toxicity effect at 72 h of post treatment.
The toxicity results of the tested weed extracts against C. chinensis at 48 h post treatment showed LC50 and LC95 values from 4.1(3.4-5.8) to 5.6(4.7-7.6) and 9.0(6.9-14.3) to 11.8(9.3-17.0) g /100 mL, respectively.
05) had significant effects on the fecundity inhibition. The treatments showed 35 to 69% fecundity inhibition and the most promising results were obtained by 4% shialmutra.
The weed species (F4,45 = 44.0, p < 0.001), extract concentrations (F2,45 = 192.1, p < 0.001) and interaction of weed species and extract concentrations (F8,45 = 2.3, p < 0.05) indicated significant effects on the adult emergence inhibitory of C. chinensis (Table 3).
참고문헌 (25)
Ambrosio, S.R., Oki, Y., Heleno, V.C.G., Chaves, J.S., Nascimento, P.G.B.D., Lichston, J.E., Constantino, M.G., Varanda, E.M., Costa, F.B., 2008. Constituents of glandular trichomes of Tithonia diversifolia: relationships to herbivory and antifeedant activity. Phytochem. 69, 2052-2060.
Amin, M.R., Shahjahan, M., El-Taj, H.F., Iqbal, T.M.T., Hossain, M.A., 2000. Use of akanda, biskatali and neem leaves as botanical insecticides against lesser grain borer. Bangladesh J. Entomol. 10, 1-13.
Cosimi, S., Rossi, E., Cioni, P.L., Canale, A., 2009. Bioactivity and qualitative analysis of some essential oils from Mediterranean plants against stored-product pests: evaluation of repellency against Sitophilus zeamais Motschulsky, Cryptolestes ferrugineus (Stephens) and Tenebrio molitor (L.). J. Stored Prod. Res. 45, 125-132.
Daglish, G., 2008. Impact of resistance on the efficacy of binary combinations of spinosad, chlorpyrifos-methyl and s-methoprene against five stored-grain beetles. J. Stored Prod. Res. 44, 1-76.
Deeba, F., Sarwar, M., Khuhro, R.D., 2006. Varietal susceptibility of mungbean genotypes to pulse beetle, Callosobruchus analis (Fabricius) (Coleoptera:Bruchidae). Pakistan J. Zool. 38, 265-268.
Fouad, H.A., Faroni, L.R.d'A., Tavares, W.S., Ribeiro, R.C., Freitas, S.S., Zanuncio, J.C., 2014. Botanical extracts of plants from the Brazilian cerrado for the integrated management of Sitotroga cerealella (Lepidoptera: Gelechiidae) in stored grain. J. Stored Prod. Res. 57, 6-11.
Kim, S.-I., Park, C., Ohh, M.H., Cho, H.-C., Ahn, Y.-J., 2003. Contact and fumigant activities of aromatic plant extracts and essential oils against Lasioderma serricorne (Coleoptera: Anobiidae). J. Stored Prod. Res. 39, 11-19.
Koona, P., Tatchago, V., Malaa, D., 2007. Impregnated bags for safer storage of legume grains in West and Central Africa. J. Stored Prod. Res. 43, 248-251.
Mishra, D., Shukla, A.K., Tripathi, K.K., Singh, A., Dixit, A.K., Singh, K., 2006. Efficacy of application of vegetable seed oils as grain protectant against infestation by Callosobruchus chinensis and its effect on milling fractions and apparent degree of dehusking of legume-pulses. J. Oleo Sci. 56, 1-7.
Mummigatti, S.G., Krishnaiah, H.E., 2007. Technique to isolate Callosobruchus chinensis (L.) (Coleoptera: Bruchidae) eggs. J. Stored Prod. Res. 43, 402-403.
Nennah, G., 2011. Toxicity and growth inhibitory activities of methanol extract and the ${\beta}$ -carboline alkaloids of Peganum harmala L. against two coleopteran stored-grain pests. J. Stored Prod. Res. 47, 255-261.
Park, I.-K., Lee, S.-G., Choi, D.-H., Park, J.-D., Ahn, Y.-J., 2003. Insecticidal activities of constituents identified in the essential oil from leaves of Chamaecyparis obtusa against Callosobruchus chinensis (L.). and Sitophilus oryzae (L). J. Stored Prod. Res. 39, 375-384.
Rahman, A., Talukder, F.A., 2006. Bioefficacy of some plant derivatives that protect grain against the pulse beetle, Callosobruchus maculatus. J. Insect Sci. 6, 1-10.
Ratnasekera, D., Nayanathara, K.H.G. 2010. Efficacy of cinnamon and Citronella oil vapors in the control of Callosobruchus chinensis L. in bulk stored green gram. J. Food. Agric. 3, 1-6.
Roy, B., Amin, M.R., Jalal, S., Kwon, Y.J., Suh, S.J., 2014. Evaluation of common cocklebur Xanthium strumarium leaf extract as post-harvest grain protectant of black gram against pulse beetle Callosobruchus chinensis (Coleoptera: Bruchidae) and isolation of crude compound. Ent. Res. 44, 254-261.
Roy, B., Jalal, S., Sarker, B.C., Amin, M.R., Jeon, Y.H., Chun, I.J., 2012b. Evaluation of insecticidal activities of common cocklebur fruit extract against pulse beetle Callosobruchus chinensis (Coleoptera: Bruchidae). Korean J. Appl. Entomol. 51, 325-329.
Roy, B., Sarker, B.C., Amin, M.R., Roy, B.C., Jalal, S., 2010. Bio-efficacy of shiyalmutra leaf extract against rice weevil. J. Sci. Technol. (Dinajpur). 8, 1-5.
Saroukolai, A.T., Moharramipour, S., Meshkatalsadat, M.H., 2010. Insecticidal properties of Thymus persicus essential oil against Tribolium castaneum and Sitophilus oryzae. J. Pest Sci. 83, 3-8.
Schneider-Orelli, O., 1947. Entomologisches Praktikum: Einfuhrung in die land- und forstwirtschaftliche Insektenkunde. Sauerlander Aarau, Germany.
Shahjahan, M., Amin, M.R., 2000. Evaluation of some plant extracts against rice weevil, Sitophilus oryzae L. J. Asiat. Soc. Bangladesh Sci. 26, 213-222.
Upadhyay, R.K., Rohatgi, L., Chaubey, M.K., Jain, S.C., 2006. Ovipositional responses of the pulse beetle, Bruchus chinensis (Coleoptera: Bruchidae) to extracts and compounds of Capparis decidua. J. Agric. Food Chem. 54, 9747-9751.
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