The composition of the essential oil of Ligustrum japonicum leaves was determined by GC-MS analysis. The major constituents of L. japonicum leaf oil were germacrene D (40.50%), ${\alpha}$-pinene (13.63%), (-)-${\beta}$-elemene (6.42%), ${\beta}$-caryophyllene (5.73%)...
The composition of the essential oil of Ligustrum japonicum leaves was determined by GC-MS analysis. The major constituents of L. japonicum leaf oil were germacrene D (40.50%), ${\alpha}$-pinene (13.63%), (-)-${\beta}$-elemene (6.42%), ${\beta}$-caryophyllene (5.73%), and $\delta$-cadinene (5.47%). The acaricidal activities of L. japonicum oil were evaluated against acaridae and pyroglyphid mites. In the fumigant bioassay, the $LD_{50}$ values of L. japonicum oil were 16.48, 12.38, and $15.63{\mu}g/cm^3$ against Tyrophagus putrescentiae, Dermatophagoides farinae, and D. pteronyssinus, respectively. In the contact bioassay, the $LD_{50}$ values of L. japonicum oil were 8.02, 5.02, and $7.67{\mu}g/cm^2$ against T. putrescentiae, D. farinae, and D. pteronyssinus, respectively.
The composition of the essential oil of Ligustrum japonicum leaves was determined by GC-MS analysis. The major constituents of L. japonicum leaf oil were germacrene D (40.50%), ${\alpha}$-pinene (13.63%), (-)-${\beta}$-elemene (6.42%), ${\beta}$-caryophyllene (5.73%), and $\delta$-cadinene (5.47%). The acaricidal activities of L. japonicum oil were evaluated against acaridae and pyroglyphid mites. In the fumigant bioassay, the $LD_{50}$ values of L. japonicum oil were 16.48, 12.38, and $15.63{\mu}g/cm^3$ against Tyrophagus putrescentiae, Dermatophagoides farinae, and D. pteronyssinus, respectively. In the contact bioassay, the $LD_{50}$ values of L. japonicum oil were 8.02, 5.02, and $7.67{\mu}g/cm^2$ against T. putrescentiae, D. farinae, and D. pteronyssinus, respectively.
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성능/효과
Compared to the LD50 values of DEET, the acaricidal potential of L. japonicum oil (12.38, 15.63, and 16.48 µg/cm3) proved to be more toxic than that of DEET (36.50, 34.23, and 30.31 µg/cm3) against D. farinae, D. pteronyssinus, and T. putrescentiae in fumigant bioassay, respectively (Table 2).
japonicum oil against three acaridae and pyroglyphid mites. In conclusion, our study showed that the essential oil of L. japonicum leaves may be useful to develop an ecofriendly agent for managing three acaridae and pyroglyphid mites.
In the contact bioassay, the acaricidal activity of L. japonicum oil (5.02, 7.67, and 8.02 µg/cm2) was more potent than that of DEET (19.64, 14.12, and 14.15 µg/cm2) against D. farinae, D. pteronyssinus, and T. putrescentiae, respectively (Table 2), indicating that D. farinae is more susceptible to L. japonicum oil than D. pteronyssinus and T. putrescentiae, regardless of the bioassays.
Moreover, the acaricidal activities of L. japonicum oil against three acaridae and pyroglyphid mites were attributed to borneol and β-caryophyllene.
Relative composition (%) of the constituents derived from L. japonicum oil was germacrene D (40.50%), α pinene (13.63%), (–)-β-elemene (6.42%), β-caryophyllene (5.73%), δ-cadinene (5.47%), α-humulene (4.46%), α-copaene (4.33%), αcadinol (3.66%), (±)-limonene (2.91%), muurolol (2.89%), αmuurolene (2.76%), β-pinene (2.34%), (+)-cycloisosativene (1.55%), myrcene (0.88%), α-amorphene (0.68%), α-terpineol (0.34%), and (–)-borneol (0.31%).
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