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문제 정의
- (14) reported that lotus leaves extracts have anti-wrinkle effects. These studies are evidence that lotus is beneficial to the skin. There has been active research on utilizing these properties to develop cosmetics for whitening and wrinkle care using lotus extracts (15-17).
제안 방법
- All the experimental procedures and protocols used in this study were re- viewed and approved by the Inje University Animal Care and Use Committee, Inje University, Gimhae, Korea. After 1 week of adjustment, the mice were randomly divided into two groups containing 10 mice each: the test group was given the lotus seed tea (LST group), and control group was given drinking water (water group) as beverages. The diet was given with the chow for both groups.
- The diet was given with the chow for both groups. After six months, the mice in each group were again divided into two groups each (n=5), one with UVB irradiation and another without UVB irradiation (water group, water-UV group, LST group, and LST-UV group). The UVB irradiation was performed for three months.
- , Glenside, PA, USA). Mice were put inside an acryl case designed for this study, and the back of the mouse was irradiated with UVB at the strength of 1.8 mW/s and 50 mJ/cm 2 three times per week. The dose of irradiation was increased by 20% every week for 15 weeks.
- The first animal experiment was performed for six months feeding chow to hairless mice. For the LST group (n=10), the lotus seed tea was provided as a drinking fluid, whereas tap water was provided for the control group (water group).
- To investigate the effects of long-term consumption of lotus seed tea on the animal skin’s moisture content, the moisture content of the skin was measured using a Corneometer CM-825.
대상 데이터
- For the experiments on the photoaging of the skin, 5-week old male SKH-1 hairless mice were purchased from Central Lab Animal, Inc. (Seoul, Korea). All animals had free access to water and market available chows for 1 week for adjustment.
데이터처리
- Different letters (a-c) on the bars indicate significant difference (P ＜0.05) by the Duncan’s multiple range test.
- One-way ANOVA, and Duncan’s multiple range test were performed to test the significance at P＜0.05 across different groups.
이론/모형
- To evaluate the extent of oxidization of skin tissues, the carbonyl content was measured according to the methods described by Oliver et al. (18), which use 2,4-dini-trophenylhydrazine (DNPH) on skin tissues. The skin homogenate was divided into 2 equal aliquots containing approximately 0.
성능/효과
- After feeding the lotus seed tea and tap water for 6 months, the mice in each group were separated into two groups (n=5), one with UVB irradiation and the other one without UVB irradiation. After 3 months, the final weight, the amount of food consumption, the amount of beverage consumption, the increase in weight, and food efficiency ratio were not significantly different across the different groups (P＜0.05) (Table 2).
- After 4 months of fluids administration, the water content of the skin in the LST group was 57.0±7.4%, and the water group showed 50.1±5.6%.
- The moisture content of the skin for the LST-UV group was 32.60±6.95%, but it was 22.67± 1.25% for the water-UV group, showing that the LSTUV group had higher moisture content than the waterUV group (P＜0.05) (Fig. 1C).
- The results show that the final weight averaged was 36.9±1.5 g for the control group and 37.2±1.7 g for the case group, with the differences not being significant.
- The results show that the moisture content of the skin was 73.53±4.68% for the LST group and 72.55±3.45% for the water group.
- There was no significant difference between water group and LST group in final body weight, food intake, water intake, body weight gain, and food efficiency.
- When the moisture content of the skin was measured after 8 months with UV irradiation, the LST-UV group showed significantly higher moisture content with 32.6±6.95%, compared to 22.67± 4.68% of the water-UV group.
후속연구
- Throughout this study, we argued that a continuous consumption of lotus seed tea would have protective effects against photoaging and regular aging. In addition, there is a needs for further research on the extent of whitening, wrinkle formation, and collagen formation for using lotus seed tea as a functional beverage with protective effects on skin against photoaging.
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