본 연구의 목적은 한국에서 재배된 70% 오이풀에탄올 추출물을 이용하여 총페놀, 총플라보노이드, 페놀릭 화합물과 항산화성(DPPH, ABTS, FRAP, 환원력)에 대해 실험결과를 제공하는 것이다. 연구결과 총페놀과 총플라보노이드의 함량은 각각 $119.3{\pm}1.54mg$gallic acid and $59.6{\pm}1.43mg$ rutin를 나타내었다. 또한, 추출물에 함유된 페놀릭 화합물의 주된 물질은 Catechin으로 나타났으며, 항산화성을 나타내는 DPPH, ABTS, FRAP, 환원력은 농도의존적으로 증가하는 경향을 나타내었다. 이상의 결과로 오이풀 추출물은 천연 항산화성 및 기능성 식품 첨가물로서 좋은 재료가 될 것으로 판단된다.
본 연구의 목적은 한국에서 재배된 70% 오이풀 에탄올 추출물을 이용하여 총페놀, 총플라보노이드, 페놀릭 화합물과 항산화성(DPPH, ABTS, FRAP, 환원력)에 대해 실험결과를 제공하는 것이다. 연구결과 총페놀과 총플라보노이드의 함량은 각각 $119.3{\pm}1.54mg$ gallic acid and $59.6{\pm}1.43mg$ rutin를 나타내었다. 또한, 추출물에 함유된 페놀릭 화합물의 주된 물질은 Catechin으로 나타났으며, 항산화성을 나타내는 DPPH, ABTS, FRAP, 환원력은 농도의존적으로 증가하는 경향을 나타내었다. 이상의 결과로 오이풀 추출물은 천연 항산화성 및 기능성 식품 첨가물로서 좋은 재료가 될 것으로 판단된다.
This study was conducted to investigate the antioxidant activity and the bioactive compounds found in 70% ethanol extracts taken from Sanguisorbae officinalis L. leaves(SO) cultivated in Korea. The extracts were tested for their total phenolic contents (TPC), total flavonoid contents (TFC), phenolic...
This study was conducted to investigate the antioxidant activity and the bioactive compounds found in 70% ethanol extracts taken from Sanguisorbae officinalis L. leaves(SO) cultivated in Korea. The extracts were tested for their total phenolic contents (TPC), total flavonoid contents (TFC), phenolic compounds, and antioxidative activities using various in vitro assay such as DPPH, ABTS radical scavenging activity, FRAP activity, and reducing power. The TPC and TFC were found to be $119.3{\pm}1.54mg$ gallic acid and $59.6{\pm}1.43mg$ rutin at mg of 70% ethanol extracts, respectively. Catechin was the major material among the phenolic compounds in SO extracts. The DPPH radical scavenging activity, ABTS radical scavenging activity, FRAP activity, and reducing power of SO extracts were increased in a dose-dependent manner. These results suggest that SO extracts could be considered as a good source of natural antioxidants and functional food ingredient.
This study was conducted to investigate the antioxidant activity and the bioactive compounds found in 70% ethanol extracts taken from Sanguisorbae officinalis L. leaves(SO) cultivated in Korea. The extracts were tested for their total phenolic contents (TPC), total flavonoid contents (TFC), phenolic compounds, and antioxidative activities using various in vitro assay such as DPPH, ABTS radical scavenging activity, FRAP activity, and reducing power. The TPC and TFC were found to be $119.3{\pm}1.54mg$ gallic acid and $59.6{\pm}1.43mg$ rutin at mg of 70% ethanol extracts, respectively. Catechin was the major material among the phenolic compounds in SO extracts. The DPPH radical scavenging activity, ABTS radical scavenging activity, FRAP activity, and reducing power of SO extracts were increased in a dose-dependent manner. These results suggest that SO extracts could be considered as a good source of natural antioxidants and functional food ingredient.
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제안 방법
The objective of this study was to investigate whether the total phenolic, total flavonoids, DPPH (2,2-diphenyl-1-pic-rylhydrazy) radical scavenging activity, ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6- sulphonic acid)) assay, ferric reducing ability of plasma (FRAP) assay, reducing power assay, major phenolic compounds of Sanguisorbae officinalis L. cultivated in Korea were evaluated by various in vitro assay and by HPLC with photodiode array detector.
대상 데이터
Folin-Ciocalteu reagent, gallic acid, rutin, catechin, vitamin C were obtained from Sigma-Aldrich (St. Louis, MO, USA). The chemicals used for antioxidant activities such DPPH (2,2-diphenyl-1-pic-rylhydrazy), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), TPTZ (2,4,6-Tripyridyl-s-triazine), trichloroacetic acid, ascorbic acid were purchased from Sigma-Aldrich (St.
The Sanguisorbae officinalis L. leaves(SO) used in this study was purchased from Daekwangherb (Chuncheon, Korea). The dried leaf sample was ground to 20～30 mesh using a grinder (IKA M 20, IKA, Staufen, Germany).
The chemicals used for antioxidant activities such DPPH (2,2-diphenyl-1-pic-rylhydrazy), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), TPTZ (2,4,6-Tripyridyl-s-triazine), trichloroacetic acid, ascorbic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA).
데이터처리
Correlation analyses were performed using the Pearson’s correlation coefficient (R).
The results were statistically analyzed by ANOVA and Duncan’s multiple range tests.
이론/모형
The ferric reducing ability of SO extract was by using the method of Benzine IFF and Strain J (1996) with modification. FRAP reagent was prepared from 300 mM acetate buffer (pH 3.
The reducing power SO extract was evaluated by the method of Jayaprakasha G et al (2001). Different concentrations (10～500 μg/mL) of SO extract in distilled water (0.
성능/효과
High correlation coefficients were found between the total flavonoid content and FRAP (r=0.975, p<0.05), between the ABTS radical scavenging activity and FRAP radical scavenging activity (r=0.957, p<0.001), and between the ABTS radical scavenging activity and reducing power activity (r=0.967, p<0.001).
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