[논문]Inactivation of various bacteriophages by different ultraviolet technologies: Development of a reliable virus indicator system for water reuse

Bae, Kyung Seon; Shin, Gwy-Am

doi:10.4491/eer.2016.032

Inactivation of various bacteriophages by different ultraviolet technologies: Development of a reliable virus indicator system for water reuse 원문보기

Environmental engineering research, v.21 no.4, 2016년, pp.350 - 354

Bae, Kyung Seon (Department of Environmental Engineering, Ajou University) , Shin, Gwy-Am (Department of Environmental Engineering, Ajou University)

Abstract ▼ AI-Helper

There is an urgent need to identify more reliable indicator systems for human pathogenic viruses in water reuse practice. In this study, we determined the response of different bacteriophages representing various bacteriophage groups to different ultraviolet (UV) technologies in real wastewater in order to identify more reliable bacteriophage indicator systems for UV disinfection in wastewater. Bacteriophage ${\varphi}X174$ PRD1, and MS2 in two different real wastewaters were irradiated with several doses of both low pressure (LP) and medium pressure (MP) UV irradiation through bench-scale UV collimated apparatus. The inactivation rate of ${\varphi}X174$ by both LP and MP UV was rapid and reached ${\sim}4{\log}_{10}$ within a UV dose of $20mJ/cm^2$. However, the inactivation rates of bacteriophage PRD1 and MS2 were much slower than the one for ${\varphi}X174$ and only ${\sim}1{\log}_{10}$ inactivation was achieved by the same UV dose of $20mJ/cm^2$. Overall, the results of this study suggest that bacteriophage MS2 could be a reliable indicator for human pathogenic viruses for both LP and MP UV disinfection in wastewater treatment processes and water reuse practice.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

문제 정의

Therefore, there is an urgent need to identify more reliable indicator systems for human pathogenic viruses in water reuse practice. In this study, we determined the response of different bacteriophages representing various bacteriophage groups to UV irradiation in both buffered system and real wastewater in order to identify more reliable bacteriophage indicator systems for UV disinfection in wastewater.
In this study, we determined the response of different bacteriophages to both traditional LP UV and alternative MP UV irradiations in real wastewaters in order to identify more reliable bacteriophage indicator systems for UV disinfection in wastewater. The results of this study showed that bacteriophage MS2 was the most resistant one to both LP and MP UV disinfection in wastewaters among the bacteriophages tested.

제안 방법

Linear regression analysis was used to calculate the IT values (UV intensity × exposure time) to achieve 2-4 log10 inactivation with EXCEL.
, Newburyport, MA). The delivered UV dose, accounting for the UV absorbance in the liquid and the depth of the suspension, was calculated based on the measurement of the irradiance incident on the petri dishes, a series of correction factors (petri factor, reflection factor, water factor, divergence factor, sensor factor (MP UV only), and germicidal factor (MP UV only)) as described in Bolton and Linden [11], and the exposure time in seconds.

데이터처리

Linear regression analysis was used to calculate the IT values (UV intensity × exposure time) to achieve 2-4 log₁₀ inactivation with EXCEL. Also, Analysis of Covariance (ANCOVA) was applied to determine the difference in the inactivation kinetics (slope difference) by different disinfectants with SAS GLM procedure.
Statistical analysis on the extent and kinetics of bacteriophage inactivation was performed by using EXCEL (Microsoft, Redmond, WA) and SAS (SAS Institute Inc., Cary, NC). Linear regression analysis was used to calculate the IT values (UV intensity × exposure time) to achieve 2-4 log₁₀ inactivation with EXCEL.

성능/효과

Overall, the results of this study indicate that bacteriophage MS2 could be a reliable indicator for human pathogenic viruses for UV disinfection in wastewater treatment processes. First, it is found relatively large numbers in wastewater.
The results of this study showed that bacteriophage φX174 was the most sensitive one to LP UV and bacteriophage MS2 was the most resistant one to LP UV, which is consistent with the ones in previous studies[5, 13, 14].
In this study, we determined the response of different bacteriophages to both traditional LP UV and alternative MP UV irradiations in real wastewaters in order to identify more reliable bacteriophage indicator systems for UV disinfection in wastewater. The results of this study showed that bacteriophage MS2 was the most resistant one to both LP and MP UV disinfection in wastewaters among the bacteriophages tested. It appears that bacteriophage MS2 could be a reliable indicator for human pathogenic viruses for both LP and MP UV disinfection in water reuse practice.
Compared to LP UV, there is little study on the inactivation of bacteriophage by MP UV. The results of this study showed that the inactivation of bacteriophages by MP UV was more extensive than LP UV, which is consistent with the one in a recent study [15].
The results of this study showed that the inactivation rates of the bacteriophage in real wastewater by LP UV were similar to (or only slightly slower than) the ones in PBS. Although there are lot of suspended matters in wastewater that may absorb or scatter UV irradiation, the correction of UV irradiance by the UV absorbance of the sample and a series of correction factors (as described in the Material and Method) appears to be appropriate for LP UV, which emits only one wavelength(254 nm).

참고문헌 (16)

World Health Organization (WHO). Wastewater reuse [Internet]. WHO; c2010. Available from: http://www.who.int/water_sanitation_health/wastewater/en/index.htm.
Environmental Protection Agency (EPA). Guidelines for water reuse. EPA/625/R-04/108. 2004. p. 93-96.
Shin G, Linden KG, Sobsey MD. Low pressure ultraviolet inactivation of pathogenic viruses and bacteriophages. J. Environ. Eng. Sci. 2005;4:S7-S11.

상세보기
Chang JC, Osoff SF, Lobe DC, Dorfman MH, Qualls RG, Johnson JD. UV inactivation of pathogenic and indicator microorganisms. Appl. Environ. Microbiol. 1985;49:1361-1365.

상세보기
Meng QS, Gerba CP. Comparative inactivation of enteric adenoviruses, polioviruses and coliphages by ultraviolet irradiation. Water Res. 1996;30:2665-2668.

상세보기
Thurston-Enriquez JA, Hass CN, Jacangelo J, Riley K, Gerba CP. Inactivation of feline calcivirus and adenovirus type 40 by UV radiation. Appl. Environ. Microbial. 2003;69:577-582.

상세보기
Sobsey MD, Batigelli DA, Handzel TR, Schwab KJ. Male-specific coliphages as indicators of viral contamination of drinking water. American Water Works Association. Denver, CO; 1995.
Adams M. Bacteriophages. New York: Interscience Publishers Inc; 1959.
Shin G, Sobsey MD. Inactivation of norovirus by chlorine disinfection of water. Water Res. 2008;42:4562-4568.

상세보기
Shin G, Lee J, Linden KG. Enhanced effectiveness of medium pressure UV on human adenovirus 2 and its possible mechanism. Water Sci. Technol. 2009;60:851-857.

상세보기
Bolton JR, Linden KG. Standardization of methods for fluence (UV dose) determination in bench-scale UV experiments. J. Environ. Eng. 2003;129:209-216.

상세보기
Shin G, Linden KG, Arrowood MJ, Sobsey MD. Low pressure UV inactivation and DNA repair potential of Cryptosporidium parvum oocysts. Appl. Environ. Microbiol. 2001;67:3029-3032.

상세보기
Nieuwstad TJ, Havelaar AH. The kinetics of batch ultraviolet inactivation of bacteriophage MS2 and microbiological calibration of an ultraviolet pilot plant. J. Environ. Sci. Health 1994;29:1993-2007.
Sommer R, Pribil W, Appelt S, et al. Inactivation of bacteriophages in water by means of non-ionizing (UV-253.7 nm) and ionizing (gamma) radiation: A comparative approach. Water Res. 2001;35:3109-3116.

상세보기
Rodriguez RA, Bounty S, Beck S, Chan C, McGuire C, Linden KG. Photoreactivation of bacteriophage after UV disinfection: Role of genome structure and impacts of UV source. Water Res. 2014;55:143-149.

상세보기
Friedberg EC, Walker GC, Siede W. DNA repair and mutagenesis. Washington D.C., USA: ASM Press; 1995.

LOADING...

원문 URL 링크

DOI : 10.4491/eer.2016.032 [무료]
대한환경공학회 : 저널
ScienceCentral : 저널
DBPia : 저널
AccessON : 저널

*원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다.

오픈액세스(OA) 유형

GOLD

오픈액세스 학술지에 출판된 논문

이 논문과 함께 이용한 콘텐츠

[논문] Viability Loss of Bacteriophage MS2 Exposed to Bronze Alloy Yugi

저작권 관리 안내

내보내기 메뉴

내보내기 구분

파일저장
인쇄
메일전송

구성항목

기본정보
상세정보

관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관

저장형식

Text(ASCII format)
Excel format
RefWorks Direct Export
RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley

메일정보

받는사람 (필수): @
보내는사람 (선택): @
제목
내용: KISTI 검색결과 이메일 서비스

안내

총 건의 자료가 검색되었습니다.

다운받으실 자료의 인덱스를 입력하세요. (1-10,000)

검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다.

데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요)

다운로드 파일은 UTF-8 형태로 저장됩니다.
파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오.

Text(ASCII format)
Excel format

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

AI-Helper ※ AI-Helper는 을 사용합니다.

AI-Helper

안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

연합인증