Objectives: The purpose of this study was to investigate the effects of low-level laser therapy (LLLT) with a diode gallium-aluminum-arsenide (Ga-Al-As) low-level laser device on the healing and attachment of titanium implants in bone. Materials and Methods: Thirteen New Zealand white male rabbits w...
Objectives: The purpose of this study was to investigate the effects of low-level laser therapy (LLLT) with a diode gallium-aluminum-arsenide (Ga-Al-As) low-level laser device on the healing and attachment of titanium implants in bone. Materials and Methods: Thirteen New Zealand white male rabbits weighing $3.0{\pm}0.5kg$ were used for this study. Dental titanium implants (3.75 mm in diameter and 8.5 mm in length, US II RBM plus fixture; Osstem, Seoul, Korea) were implanted into both femurs of each rabbit. The rabbits were randomly divided into a LLLT group and a control group. The LLLT was initiated immediately after surgery and then repeated daily for 7 consecutive days in the LLLT group. Six weeks and 12 weeks after implantation, we evaluated and compared the osseointegration of the LLLT group and control group, using histomorphometric analysis, removal torque testing, and resonance frequency analysis (RFA). The results were statistically significant when the level of probability was 0.05 or less based on a non-parametric Mann-Whitney U-test. Results: The implant survival rate was about 96%. Histologically and histomorphometrically, we observed that the titanium implants were more strongly attached in LLLT group than in control group. However, there was no significant difference between the LLLT group and control group in removal torque or RFA. Conclusion: Histologically, LLLT might promote cell-level osseointegration of titanium implants, but there was no statistically significant effects.
Objectives: The purpose of this study was to investigate the effects of low-level laser therapy (LLLT) with a diode gallium-aluminum-arsenide (Ga-Al-As) low-level laser device on the healing and attachment of titanium implants in bone. Materials and Methods: Thirteen New Zealand white male rabbits weighing $3.0{\pm}0.5kg$ were used for this study. Dental titanium implants (3.75 mm in diameter and 8.5 mm in length, US II RBM plus fixture; Osstem, Seoul, Korea) were implanted into both femurs of each rabbit. The rabbits were randomly divided into a LLLT group and a control group. The LLLT was initiated immediately after surgery and then repeated daily for 7 consecutive days in the LLLT group. Six weeks and 12 weeks after implantation, we evaluated and compared the osseointegration of the LLLT group and control group, using histomorphometric analysis, removal torque testing, and resonance frequency analysis (RFA). The results were statistically significant when the level of probability was 0.05 or less based on a non-parametric Mann-Whitney U-test. Results: The implant survival rate was about 96%. Histologically and histomorphometrically, we observed that the titanium implants were more strongly attached in LLLT group than in control group. However, there was no significant difference between the LLLT group and control group in removal torque or RFA. Conclusion: Histologically, LLLT might promote cell-level osseointegration of titanium implants, but there was no statistically significant effects.
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문제 정의
This research was conducted to analyze the effects of LLLT on osseointegration after placement of titanium implants in rabbit femurs and application of LLL. The LLLT had a positive effect on osseointegration at a cellular level.
제안 방법
After the sixth and 12th weeks of implant placement, rabbits from each group were sacrificed, and their femoral condyle was amputated for extraction. The extracted tissues were fixed in 10% neutral buffered formalin for six weeks.
In this research, we performed an animal study to determine if in vivo application of LLLT effectively stimulates osseointegration of dental implants. We performed histomorphometric analysis, removal torque testing, and resonance frequency analysis (RFA) to evaluate dental implant osseointegration and to ensure clinical results.
RFA can measure early implant stability and regularly determine the status of an implant. The peak removal torque value measured by a digital torque machine is used for removal torque testing, and the implant stability quotient (ISQ) was statistically analyzed in this study for RFA.
Similarly, results of research that examine the effects of LLLT on osseointegration vary, and the application of LLLT to clinical practices is still debated. This research study analyzed the effects of LLLT on osseointegration through histopathological analysis and by assessing the degree of osseointegration in an animal model.
대상 데이터
The main effect of LLLT is non-exothermic in vivo stimulation, and no side effects have been reported. The LLL wavelength is between 500 to 1,200 nm, and a Ga-As-Al diode laser, which was used in this study, has a wavelength of 808 nm. The Ga-As-Al diode laser has been found to have a better penetration effect than any other lasers.
LLLT group was treated with LLLT for one week after the surgery, and the other was designated as the control group, which was not treated with LLLT. Twenty-six dental titanium implants (US II RBM plus fixture; Osstem, Seoul, Korea) were used, and a diode galliumaluminum-arsenide (Ga-Al-As) laser device (IMPRA-ORT; NDLUX, Anyang, Korea) was used for LLLT.
데이터처리
To determine the difference in osseointegration between the LLLT group and control group, BIC ratio values at the sixth and 12th weeks, removal torque values, and dif. % ISQ values were evaluated through non-parametric Mann Whitney U-testing (PASW Statistics for Windows version 18.0; IBM Co., Armonk, NY, USA) to determine the statistical significance. A P-value less than or equal to 0.
이론/모형
Osseous tissues to the third screw thread of each implant were observed with the light microscope (×100, Carl Zeiss Microscopy GmbH). The Zen 2011 program (Carl Zeiss Microscopy GmbH) was used to calculate the values and the ratios.
성능/효과
In histomorphometric analysis, the average BIC ratio values of the control group and the LLLT group at the sixth week were 52.1%±18.3% and 55.4%±9.0%, respectively, while those at the 12th week were 50.4%±9.8% and 62.2%±10.2%.
In this research, the average ISQ values for the six-week groups were 68.3±15.5 and 69.0±10.0, respectively, and the average removal torque values were 60.0±32.5 cNm and 54.1±4.3 cNm.
The average BIC ratio values of the control group and the LLLT group at 12 weeks were 50.4%±9.8% and 62.2%±10.2%, respectively.
후속연구
Additionally, using experimental animals whose osseous tissues are similar to human maxillary bone is necessary for optimal assessment and comparison. Altogether, the results of this study are promising, although further in-depth research will be essential to determine whether LLLT has positive effects on osseointegration.
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