[논문]Laser Speckle Contrast Imaging for Measuring Cerebral Blood Flow Changes Caused by Electrical Sensory Stimulation

Cho, Ahra; Yeon, Chanmi; Kim, Donghyeon; Chung, Euiheon

doi:10.3807/josk.2016.20.1.088

Laser Speckle Contrast Imaging for Measuring Cerebral Blood Flow Changes Caused by Electrical Sensory Stimulation 원문보기

Journal of the Optical Society of Korea, v.20 no.1, 2016년, pp.88 - 93

Cho, Ahra (School of Mechatronics, Gwangju Institute of Science and Technology) , Yeon, Chanmi (Department of Medical System Engineering, Gwangju Institute of Science and Technology) , Kim, Donghyeon (School of Information and Communications, Gwangju Institute of Science and Technology) , Chung, Euiheon (School of Mechatronics, Gwangju Institute of Science and Technology)

Abstract ▼ AI-Helper

Recently laser speckle contrast (LSC) imaging has become a widely used optical method for in vivo assessment of blood flow in the animal brain. LSC imaging is useful for monitoring brain hemodynamics with relatively high spatio-temporal resolution. A speckle contrast imaging system has been implemented with electrical sensory stimulation apparatus. LSC imaging is combined with optical intrinsic signal imaging in order to measure changes in cerebral blood flow as well as neural activity in response to electrical sensory stimulation applied to the hindlimb region of the mouse brain. We found that blood flow and oxygen consumption are correlated and both sides of hindlimb activation regions are symmetrically located. This apparatus could be used to monitor spatial or temporal responses of cerebral blood flow in animal disease models such as ischemic stroke or cortical spreading depression.

주제어

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문제 정의

This study demonstrates that the combined imaging of laser speckle contrast and intrinsic optical signal can provide correlative information with electrical sensory stimulation of a limb towards preclinical stroke research. To induce hemodynamic response by functional activation, an electrical sensory stimulation apparatus was built and synchronized with camera acquisition.

가설 설정

Fig. 6. The starting times of speckle contrast and intrinsic signals right after electrical stimulation are comparable. Subsequent to sensory stimulation, the cerebral blood flow (speckle contrast) starts to increase at approximately 1.

제안 방법

In this paper, we delineated optimal stimulation parameters to maximizing laser speckle contrast with a home-built laser speckle contrast (LSC) imaging setup, and applied it for measuring cerebral blood flow changes in response to electrical sensory stimulation with an experimental mouse model. The comparison with optical intrinsic signal (OIS) imaging demonstrates an exemplary correlation of blood flow and neural activity-dependent changes.

대상 데이터

uk/arrive-guidelines). C₅₇BL/6 mice with cranial windows were used for experiments. Mice with 25-30 g body weight and ages of 8-10 weeks were anesthetized with Zoletil/Xylazine mixture in saline solution (60/10 ㎎/㎏ body weight).
The laser speckle imaging instrumentation consists of a _helium-neon_{laser (}λ_{= 632.8 nm, 17 mW, Research Electro-} Optics, Inc., Colorado USA), a scientific CMOS camera (Neo 5.5 sCMOS, Andor Technology Ltd., Belfast, UK), and a microscope body (BX-51, Olympus Co.) having an objective lens (RMS4X, NA 0.10, Olympus) as shown in Fig. 1. The laser beam was coupled into a single mode fiber (core diameter = 4.

성능/효과

) was also activated in LSC imaging which is indicated by the yellow box in the Fig. 4. Especially, a peak of relative increase in speckle contrast due to cerebral blood flow was over 5%. The time course of the contrast changes averaged in the ROI that is centered over the activated area is shown in Fig.

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