[논문]Characterization of Two New Records of Mucoralean Species Isolated from Gut of Soldier Fly Larva in Korea

Nguyen, Thi Thuong Thuong; Duong, Tham Thi; Lee, Hyang Burm

doi:10.5941/myco.2016.44.4.310

Characterization of Two New Records of Mucoralean Species Isolated from Gut of Soldier Fly Larva in Korea 원문보기

Mycobiology, v.44 no.4, 2016년, pp.310 - 313

Nguyen, Thi Thuong Thuong (Division of Food Technology, Biotechnology & Agrochemistry, College of Agriculture & Life Sciences, Chonnam National University) , Duong, Tham Thi (Division of Food Technology, Biotechnology & Agrochemistry, College of Agriculture & Life Sciences, Chonnam National University) , Lee, Hyang Burm (Division of Food Technology, Biotechnology & Agrochemistry, College of Agriculture & Life Sciences, Chonnam National University)

Abstract ▼ AI-Helper

While surveying the diversity of fungi of the order Mucorales, two isolates, EML-PUKI12-1 and EML-PUKI06-1, were obtained from the gut of soldier fly larvae inhabiting the bulrush at a pond located in the Chonnam National University Arboretum, Gwangju, Korea. The isolates were confirmed as Mucor irregularis and Mucor fragilis species, respectively, based on the morphological characteristics and phylogenetic analysis of rDNA internal transcribed spacer region. Such mucoralean species belonging to undiscovered taxa has not previously been described in Korea.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

제안 방법

The plates were incubated at 10^oC, 20^oC, 25^oC, 30^oC, and 35^oC in the dark for 7 days. Samples were mounted in lactophenol solution (Junsei Chemical Co. Ltd., Tokyo, Japan) and observed using an Olympus BX51 microscope with DIC optics (Olympus, Tokyo, Japan).
The objective of the present study was to perform the morphological and molecular analyses to characterize two unrecorded mucoralean species—Mucor irregularis and Mucor fragilis in Korea.
). The plates were incubated at 10^oC, 20^oC, 25^oC, 30^oC, and 35^oC in the dark for 7 days. Samples were mounted in lactophenol solution (Junsei Chemical Co.

대상 데이터

Phylogenetic trees were constructed from the data using maximum likelihood. The ITS sequences of EMLPUKI12-1, EML-PUKI12-2 and EML-PUKI06-1, EMLPUKI06-2 strains were deposited in the GenBank database with accession numbers (KY047151, KY047146, KY047147, and KY047150, respectively). A BLASTn search revealed that the rDNA ITS homology of EML-PUKI12-1 and EMLPUKI06-1 represented 99.

성능/효과

The ITS sequences of EMLPUKI12-1, EML-PUKI12-2 and EML-PUKI06-1, EMLPUKI06-2 strains were deposited in the GenBank database with accession numbers (KY047151, KY047146, KY047147, and KY047150, respectively). A BLASTn search revealed that the rDNA ITS homology of EML-PUKI12-1 and EMLPUKI06-1 represented 99.1% (572/577 bp) and 99.2% (610/615 bp) sequence identity values with M. irregularis (GenBank accession No. JX975255) and M. fragilis (GenBank accession No. FN650655). The phylogenetic tree of the ITS region with a high bootstrap value (Fig.
FN650655). The phylogenetic tree of the ITS region with a high bootstrap value (Fig. 1), indicated that the isolates EML-PUKI12-1 and EML-PUKI06-1 were identical to M. irregularis and M. fragilis.
To confirm the molecular phylogenetic result, the morphology and growth rate of the isolates EML-PUKI12-1 and EML-PUKI06-1 were determined. Cultural features were observed on PDA, synthetic mucor agar (SMA; 40 g dextrose, 2 g asparagine, 0.

후속연구

Although some kinds of new and undescribed zygomycetous fungi in Korea have been reported in several recent studies, data relating to the diversity of zygomycetous fungi in Korea are still lacking. Therefore, M. irregularis and M. fragilis isolated from the gut of insects and described as new record can contribute to the knowledge of diversity of zygomycetous fungi in Korea.

참고문헌 (18)

1 Micheli PA Nova plantarum genera juxta Tournefortii methodum disposita Florence Typis Bernardi Paperinii 1729
2 Schipper MA On certain species of Mucor with a key to all accepted species Stud Mycol 1978 17 1 52
3 Benny GL Methods used by Dr. R. K. Benjamin, and other mycologists, to isolate zygomycetes Aliso 2008 26 37 61
4 Li GJ Hyde KD Zhao RL Hongsanan S Abdel-Aziz FA Abdel-Wahab MA Alvarado P Alves-Silva G Ammirati JF Ariyawansa HA Fungal diversity notes 253-366: taxonomic and phylogenetic contributions to fungal taxa Fungal Divers 2016 78 1 237
5 Nguyen TT Lee SH Bae S Jeon SJ Mun HY Lee HB Characterization of two new records of zygomycete species belonging to undiscovered taxa in Korea Mycobiology 2016 44 29 37 27103852

원문보기 상세보기
6 Jacobs K Botha A Mucor renisporus sp. nov., a new coprophilous species from Southern Africa Fungal Divers 2008 29 27 35
7 Walther G Pawłowska J Alastruey-Izquierdo A Wrzosek M Rodriguez-Tudela JL Dolatabadi S Chakrabarti A de Hoog GS DNA barcoding in Mucorales: an inventory of biodiversity Persoonia 2013 30 11 47 24027345

상세보기
8 Alves MH Campos-Takaki GM Porto AL Milanez AI Screening of Mucor spp. for the production of amylase, lipase, polygalacturonase and protease Braz J Microbiol 2002 33 325 330
9 Kendrick A Ratledge C Desaturation of polyunsaturated fatty acids in Mucor circinelloides and the involvement of a novel membrane-bound malic enzyme Eur J Biochem 1992 209 667 673 1425673

상세보기
10 Ahn GR Kwon HW Ko HK Kim SH Unrecorded fungal species isolated from greenhouses used for shiitake cultivation in Korea Kor J Mycol 2016 44 8 15
11 Lee YS Jung HY Lee HB Kim SH Shin KS Eom AH Kim C Lee SY Korean Society of Mycology National list of species of Korea. Ascomycota, Glomeromycota, Zygomycota, Myxomycota, Oomycota Incheon National Institute of Biological Resources 2015
12 White TJ Bruns T Lee S Taylor J Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics Innis MA Gelfand DH Sninsky JJ White TJ PCR protocols: a guide to methods and applications San Diego (CA) Academic Press 1990 315 322
13 Thompson JD Gibson TJ Plewniak F Jeanmougin F Higgins DG The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools Nucleic Acids Res 1997 25 4876 4882 9396791

상세보기
14 Hall TA BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT Nucleic Acids Symp Ser 1999 41 95 98

상세보기
15 Tamura K Stecher G Peterson D Filipski A Kumar S MEGA6: molecular evolutionary genetics analysis version 6.0 Mol Biol Evol 2013 30 2725 2729 24132122

상세보기
16 Lu XL Najafzadeh MJ Dolatabadi S Ran YP Gerrits van den Ende AH Shen YN Li CY Xi LY Hao F Zhang QQ Taxonomy and epidemiology of Mucor irregularis , agent of chronic cutaneous mucormycosis Persoonia 2013 30 48 56 24027346

상세보기
17 Bainier M Nouvelles observations sur les zygospores des Mucorinées Ann Sci Nat Bot 1884 19 200 214
18 Christenberry GA A taxonomic study of the Mucorales in the Southeastern United States J Elisha Mitchell Sci Soc 1940 56 333 366

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Characterization of Two New Records of Mucoralean Species Isolated from Gut of Soldier Fly Larva in Korea 원문보기

Abstract ▼ AI-Helper

주제어

AI 본문요약
AI-Helper

제안 방법

대상 데이터

성능/효과

후속연구

참고문헌 (18)

이 논문을 인용한 문헌

저자의 다른 논문 :

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

연합인증

Characterization of Two New Records of Mucoralean Species Isolated from Gut of Soldier Fly Larva in Korea 원문보기

Abstract ▼ AI-Helper

주제어

AI 본문요약 엑셀 다운로드 AI-Helper

제안 방법

대상 데이터

성능/효과

후속연구

참고문헌 (18)

이 논문을 인용한 문헌

저자의 다른 논문 :

Nguyen, Thi Thuong Thuong (9) Duong, Tham Thi (1) 이향범 (44)

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

AI 본문요약
AI-Helper