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제안 방법
- Epilithic biofilm biomass was examined using a combination of direct cell counts and indirect chlorophyll a extraction method. Furthermore, biofilm biomass was non-destructively quantified by spectral reflectance using the normalized difference vegetation index (NDVI) and a simple vegetation index (VI) in order to evaluate the possibility of using remote-sensing technique.
- The aims of the current study were to examine the community structure and abundance of epilithic biofilms along shore gradients and to detect seasonal patterns of biofilm biomass and taxonimical composition at two study sites with different degrees of wave exposure. Epilithic biofilm biomass was examined using a combination of direct cell counts and indirect chlorophyll a extraction method.
- To examine temporal-spatial variation in the chlorophyll a content and the correlations between vegetation indices (NDVI or VI) and chlorophyll a, a total of 48 rock chips (eight replicates on each shore level at two study sites) were measured for reflectance followed by chlorophyll a extraction. This was a necessary calibration step to be able to use the reflectance indices to trace seasonal and vertical changes in the epilithic microalgal biomass.
대상 데이터
- 20℃). At each sampling date, a total of 66 rock chips, including 44 chips for chlorophyll a extraction and 18 chips for community structure analyses, were sampled.
- Figure 4. Seasonal variations of relative abundance of the major taxonomic groups of three intertidal shore levels at the two study sites of Byeonsan Peninsula, Korea, from January to September 2011. Data showed as mean values of three replicates
데이터처리
- 0 software. A one-way ANOVA (analysis of variance) was used to test the difference in biofilm biomass between the two sites over the study period. For each a site, two-way ANOVA was used to determine the difference in biofilm biomass between the season and tidal levels.
- A one-way ANOVA (analysis of variance) was used to test the difference in biofilm biomass between the two sites over the study period. For each a site, two-way ANOVA was used to determine the difference in biofilm biomass between the season and tidal levels. The significance of the differences between mean values was evaluated with the Tukey HSD test (Sokal and Rohlf, 1981).
이론/모형
- The aims of the current study were to examine the community structure and abundance of epilithic biofilms along shore gradients and to detect seasonal patterns of biofilm biomass and taxonimical composition at two study sites with different degrees of wave exposure. Epilithic biofilm biomass was examined using a combination of direct cell counts and indirect chlorophyll a extraction method. Furthermore, biofilm biomass was non-destructively quantified by spectral reflectance using the normalized difference vegetation index (NDVI) and a simple vegetation index (VI) in order to evaluate the possibility of using remote-sensing technique.
- Reflectance spectra were measured on eight rock chips at each shore level. Reflectance measurements were used to estimate epilithic biofilm biomass by calculating the normalized difference vegetation index (NDVI, Rouse et al., 1973) and vegetation index (VI, Jordan, 1969). The NDVI and VI were calculated as follows (Jordan, 1969; Rouse et al.
- Rock chips with epilithic biofilms were collected bimonthly from the intertidal zone of Gosapo (35°39′N, 126°30′E) and Gyeokpo (35°38′N, 126°27′E), Byeonsan, Korea, from November 2010 to September 2011. The levels of wave exposure at the two study sites were measured using a dynamometer, which was made by the following protocol of Bell and Denny(1994). Relative levels of wave exposure, from zero (no movement) to 1 (full tie length), were calculated using the moving distance of the rubber indicator connected to the practice golf ball with a nylon cable tie.
- For each a site, two-way ANOVA was used to determine the difference in biofilm biomass between the season and tidal levels. The significance of the differences between mean values was evaluated with the Tukey HSD test (Sokal and Rohlf, 1981). Cochran's test was used to verify homoscedasticity, and data transformations were applied when necessary.
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