[논문]Fermented Acanthopanax koreanum Root Extract Reduces UVB- and H2O2-Induced Senescence in Human Skin Fibroblast Cells

Park, Min-Ja; Bae, Young-Seuk

doi:10.4014/jmb.1602.02049

Fermented Acanthopanax koreanum Root Extract Reduces UVB- and H2O2-Induced Senescence in Human Skin Fibroblast Cells 원문보기

Journal of microbiology and biotechnology, v.26 no.7, 2016년, pp.1224 - 1233

Park, Min-Ja (School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University) , Bae, Young-Seuk (School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University)

Abstract ▼ AI-Helper

The present study assessed the effects of an aqueous extract of Acanthopanax koreanum root (AE) and of AE following fermentation by lactic acid bacteria (Lactobacillus plantarum and Bifidobacterium bifidum) (AEF) on human skin fibroblast HS68 cells exposed to ultraviolet B (UVB) irradiation and oxidative stress. AEF effectively antagonized the senescence-associated β-galactosidase staining and upregulation of p53 and p21Cip1/WAF1 induced by UVB or H2O2 treatment in HS68 cells. It also exhibited excellent antioxidant activities in radical scavenging assays and reduced the intracellular level of reactive oxygen species induced by UVB or H2O2 treatment. The antioxidant and antisenescent activities of AEF were greater than those of nonfermented A. koreanum extract. AEF significantly repressed the UVB- or H2O2-induced activities of matrix metalloproteinase (MMP)-1 and -3, overexpression of MMP-1, and nuclear factor κB (NF-κB) activation. This repression of NF-κB activation and MMP-1 overexpression was attenuated by a mitogen-activated protein kinase activator, suggesting that this AEF activity was dependent on this signaling pathway. Taken together, these data indicated that AEF-mediated antioxidant and anti-photoaging activities may produce anti-wrinkle effects on human skin.

주제어

AI 본문요약
AI-Helper

제안 방법

Intracellular ROS were measured as described previously [18], using the oxidation-sensitive fluorescent probes dichlorofluorescein diacetate (DCFDA; Invitrogen, USA) and dihydroethidium (DHE; Invitrogen, USA).

대상 데이터

The human foreskin fibroblast cell line HS68 was obtained from the American Type Culture Collection (ATCC, USA). HS68 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% (v/v) fetal bovine serum.
A. koreanum roots were purchased from an herbal medicine shop located in Jeju Island, South Korea. They were cut, washed several times using distilled water, and air-dried naturally in a cool, dark environment.

이론/모형

plantarum-fermented AE and B. bifidum-fermented AE (1:2) was used in this study.

성능/효과

We found that AE successfully suppressed the intracellular ROS production (Figs. 3A and 3B) induced by UVB irradiation and H2O2 treatment in HS68 cells, but its effects on SA-β-gal activity and expression of p53 and p21Cip1/WAF1 were negligible (Figs. 1 and 2).
3A and 3B) in HS68 cells treated with UVB or H2O2, as compared with AE. Analysis of DPPH and ABTS radical scavenging capacity also indicated that AEF had greater antioxidant activity than AE (Figs. 3C and 3D).
In this study, we found that AE suppressed the enhanced activity of MMP-1 and -3 and the overexpression of MMP-1 in dermal fibroblast cells treated with UVB or H2O2. Consistently, AEF exhibited greater reduction of UVB- or H2O2-induced activation of MMP-1 and -3 and MMP-1 overexpression than did AE (Fig. 4).
In conclusion, we have identified a powerful agent for the protection of human skin cells from UVB irradiation and oxidative stress. The present study indicated for the first time that AEF exhibited greater antioxidant and anti-photoaging capacities than AE.
5A and 5B). Taken together, these results suggest that the effect of AEF on UVB- or ROS-mediated MMP-1 upregulation is, at least partly, dependent on the p38 MAPK-NF-κB signaling pathway in dermal fibroblast cells (Fig. 6).
This study showed that both UVB irradiation and H2O2 treatment produced comparable stimulation of aging-related biological markers, such as an increase in SA-β-gal activity and p53 and p21Cip1/WAF1 protein levels, and generated ROS in human dermal fibroblast HS68 cells. We found that AE successfully suppressed the intracellular ROS production (Figs.

후속연구

However, the mechanism underlying the enhanced anti-photoaging capacity of AEF remains to be elucidated. Studies to isolate and identify the compounds in AEF that exert anti-photoaging effects will be carried out in the future.

참고문헌 (32)

Akazaki S, Nakagawa H, Kazama H, Osanai O, Kawai M, Takema Y, Imokawa G. 2002. Age-related changes in skin wrinkles assessed by a novel three-dimensional morphometric analysis. Br. J. Dermatol. 147: 689-695.

상세보기
Al-Mohanna MA, Al-Khodairy FM, Krezolek Z, Bertilsson PA, Al-Houssein KA, Aboussekhra A. 2001. p53 is dispensable for UV-induced cell cycle arrest at late G1 in mammalian cells. Carcinogenesis 22: 573-578.

상세보기
Bell S, Degitz K, Quirling M, Jilg N, Page S, Brand K. 2003. Involvement of NF-κB signalling in skin physiology and disease. Cell. Signal. 15: 1-7.

상세보기
Bickers DR, Athar M. 2006. Oxidative stress in the pathogenesis of skin disease. J. Invest. Dermatol. 126: 2565-2575.

상세보기
Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200.

상세보기
Bond M, Baker AH, Newby AC. 1999. Nuclear factor kappaB activity is essential for matrix metalloproteinase-1 and -3 upregulation in rabbit dermal fibroblasts. Biochem. Biophys. Res. Commun. 264: 561-567.

상세보기
Cadet J, Wagner JR. 2013. DNA base damage by reactive oxygen species, oxidizing agents, and UV radiation. Cold Spring Harb. Perspect. Biol. 5: a012559.
Cai XF, Lee IS, Shen G, Dat NT, Lee JJ, Kim YH. 2004. Triterpenoids from Acanthopanax koreanum root and their inhibitory activities on NFAT transcription. Arch. Pharm. Res. 27: 825-828.

원문보기 상세보기
Chen L, Hu JY, Wang SQ. 2012. The role of antioxidants in photoprotection: a critical review. J. Am. Acad. Dermatol. 67: 1013-1024.

상세보기
Di Girolamo N, Coroneo MT, Wakefield D. 2003. UVB-elicited induction of MMP-1 expression in human ocular surface epithelial cells is mediated through the ERK1/2 MAPK-dependent pathway. Invest. Ophthalmol. Vis. Sci. 44: 4705-4714.

상세보기
Di Marzio L, Cinque B, Cupelli F, De Simone C, Cifone M, Giuliani M. 2008. Increase of skin-ceramide levels in aged subjects following a short-term topical application of bacterial sphingomyelinase from Streptococcus thermophilus. Int. J. Immunopathol. Pharmacol. 21: 137-143.

상세보기
Farage MA, Miller KW, Elsner P, Maibach HI. 2008. Intrinsic and extrinsic factors in skin ageing: a review. Int. J. Cosmet. Sci. 30: 87-95.

상세보기
Fiore R, Miller R, Coffman SM. 2013. Mycobacterium mucogenicum infection following a cosmetic procedure with poly-L-lactic acid. J. Drugs Dermatol. 12: 353-357.
Fisher GJ, Datta SC, Talwar HS, Wang ZQ, Varani J, Kang S, Voorhees JJ. 1996. Molecular basis of sun-induced premature skin ageing and retinoid antagonism. Nature 379: 335-339.

상세보기
Goldstein S. 1990. Replicative senescence: the human fibroblast comes of age. Science 249: 1129-1133.

상세보기
Hahn DR, Kim CJ, Kim JH. 1985. A study on the chemical constituents of Acanthopanax koreanum Nakai and its pharmaco-biological activities. Yakhak Hoeji 29: 357-361.
Jenkins G. 2002. Molecular mechanisms of skin ageing. Mech. Ageing Dev. 123: 801-810.

상세보기
Jeon SM, Lee SJ, Kwon TK, Kim KJ, Bae YS. 2010. NADPH oxidase is involved in protein kinase CKII down-regulation-mediated senescence through elevation of the level of reactive oxygen species in human colon cancer cells. FEBS Lett. 584: 3137-3142.

상세보기
Lewis DA, Spandau DF. 2008. UVB-induced activation of NF-kB is regulated by the IGF-1R and dependent on p38 MAPK. J. Invest. Dermatol. 128: 1022-1029.

상세보기
Lin QY, Jin LJ, Cao ZH, Xu YP. 2008. Inhibition of inducible nitric oxide synthase by Acanthopnax senticosus extract in RAW264.7 macrophages. J. Ethnopharmacol. 118: 231-236.

상세보기
Liou GY, Storz P. 2010. Reactive oxygen species in cancer. Free Radic. Res. 44: 479-496.

상세보기
Nhiem NX, Kim KC, Kim AD, Hyun JW, Kang HK, Van Kiem P, et al. 2011. Phenylpropanoids from the leaves of Acanthopanax koreanum and their antioxidant activity. J. Asian Nat. Prod. Res. 13: 56-61.

상세보기
Poljšak B, Fink R. 2014. The protective role of antioxidants in the defence against ROS/RNS-mediated environmental pollution. Oxid. Med. Cell. Longev. 2014: 671539.

상세보기
Poon RY, Jiang W, Toyoshima H, Hunter T. 1996. Cyclin-dependent kinases are inactivated by a combination of p21 and Thr-14/Tyr-15 phosphorylation after UV-induced DNA damage. J. Biol. Chem. 271: 13283-13291.

상세보기
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 2: 1231-1237.

상세보기
Rittié L, Fisher GJ. 2002. UV-light-induced signal cascades and skin aging. Ageing Res. Rev. 1: 705-720.

상세보기
Takema Y, Yorimoto Y, Kawai M. 1995. The relationshipbetween age-related changes in the physical properties and development of wrinkles in human facial skin. J. Soc. Cosmet. Chem. 46: 163-173.
Tsatsou F, Trakatelli M, Patsatsi A, Kalokasidis K, Sotiriadis D. 2012. Extrinsic aging: UV-mediated skin carcinogenesis. Dermatoendocrinol. 4: 285-297.

상세보기
Watson RE, Gibbs NK, Griffiths CE, Sherratt MJ. 2014. Damage to skin extracellular matrix induced by UV exposure. Antioxid. Redox Signal. 21: 1063-1077.

상세보기
Wu YL, Jiang YZ, Jin XJ, Lian LH, Piao JY, Wan Y, et al. 2010. Acanthoic acid, a diterpene in Acanthopanax koreanum, protects acetaminophen-induced hepatic toxicity in mice. Phytomedicine 17: 475-479.

상세보기
Yang EJ, Moon JY, Lee JS, Koh J, Lee NH, Hyun CG. 2010. Acanthopanax koreanum fruit waste inhibits lipopolysaccharide-induced production of nitric oxide and prostaglandin E2 in RAW 264.7 macrophages. J. Biomed. Biotechnol. 2010: 715739.

상세보기
Yang HS, Choi YJ, Oh HH, Moon JS, Jung HK, Kim KJ, et al. 2014. Antioxidative activity of mushroom water extracts fermented by lactic acid bacteria. J. Korean Soc. Food Sci. Nutr. 43: 80-85.

원문보기 상세보기

저자의 다른 논문 :

LOADING...

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증