Severe complications associated with EV71 infections are a common cause of neonatal death. Lack of effective therapeutic agents for these infections underlines the importance of research for the development of new antiviral compounds. In the present study, the anti-EV71 activity of norwogonin, oroxy...
Severe complications associated with EV71 infections are a common cause of neonatal death. Lack of effective therapeutic agents for these infections underlines the importance of research for the development of new antiviral compounds. In the present study, the anti-EV71 activity of norwogonin, oroxylin A, and mosloflavone from Scutellaria baicalensis Georgi was evaluated using a cytopathic effect (CPE) reduction method, which demonstrated that all three compounds possessed strong anti-EV71 activity and decreased the formation of visible CPEs. Norwogonin, oroxylin A, and mosloflavone also inhibited virus replication during the initial stage of virus infection, and they inhibited viral VP2 protein expression, thereby inhibiting viral capsid protein synthesis. However, ribavirin has a relatively weaker efficacy compared to the other drugs. Therefore, these findings provide important information that will aid in the utilization of norwogonin, oroxylin A, and mosloflavone for EV71 treatment.
Severe complications associated with EV71 infections are a common cause of neonatal death. Lack of effective therapeutic agents for these infections underlines the importance of research for the development of new antiviral compounds. In the present study, the anti-EV71 activity of norwogonin, oroxylin A, and mosloflavone from Scutellaria baicalensis Georgi was evaluated using a cytopathic effect (CPE) reduction method, which demonstrated that all three compounds possessed strong anti-EV71 activity and decreased the formation of visible CPEs. Norwogonin, oroxylin A, and mosloflavone also inhibited virus replication during the initial stage of virus infection, and they inhibited viral VP2 protein expression, thereby inhibiting viral capsid protein synthesis. However, ribavirin has a relatively weaker efficacy compared to the other drugs. Therefore, these findings provide important information that will aid in the utilization of norwogonin, oroxylin A, and mosloflavone for EV71 treatment.
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제안 방법
After incubation at 37°C in 5% CO2 for 48 h, the cell viability was evaluated using the sulforhodamine B (SRB) assay, and the morphology of cells was determined using a microscope.
대상 데이터
Tissue culture plates were purchased from Falcon (BD Biosciences, Franklin Lakes, NJ, USA). Ribavirin and sulforhodamine B were purchased from Sigma-Aldrich (St Louis, MO, USA).
데이터처리
To compare multiple groups, we performed one-way ANOVA followed by the Tukey post hoc test using GraphPad Prism version 5 software (Graphpad, San Diego, CA, USA). Values of p<0.
one-way analysis of variance (ANOVA) with Tukey’s post hoc test.
이론/모형
Membranes were developed by the enhanced chemiluminescence (ECL) method using West-Q chemiluminescent substrate (GenDEPOT).
4, 2, 10, and 50 mg/mL) of norwogonin, oroxylin A, mosloflavone for 48 h. The antiviral activity was investigated using a CPE reduction assay. Data are presented as mean ± SD.
성능/효과
The effect of norwogonin, oroxylin A, and mosloflavone on each stage of the EV71 infection cycle and the treatment period was assessed to observe the antiviral activity. From the results, pre-incubation of Vero cells with norwogonin, oroxylin A, and mosloflavone did not protect the cells from EV71 infection (Fig. 3). Furthermore, norwogonin, oroxylin A, and mosloflavone only inhibited EV71 virus infection when added at 1, 2, and 4 h after virus inoculation (Fig.
Norwogonin, oroxylin A, and mosloflavone were added at different time points (before, during, and after) of EV71 infection. The results showed that norwogonin, oroxylin A, and mosloflavone suppressed EV71 infection when added just after virus inoculation (0 h) and during the early stages after virus inoculation (1, 2, and 4 h), and the effect decreased when they were added just after virus inoculation (8 h). The inhibitory levels of norwogonin, oroxylin A, and mosloflavone were higher than 80% (Fig.
후속연구
Further studies will be required to explore the detailed antiviral mechanism of action of norwogonin, oroxylin A, and mosloflavone known as flavonoid. We will carry out research focusing on suppression of enterovirus replication by norwogonin, oroxylin A, and mosloflavone because baicalin, a flavonoid derived from Scutellaria baicalensis, has been demonstrated that it is inhibited viral nucleotide synthesis against dengue virus (Moghaddam et al.
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