[논문]Fructus Corni Officinalis water extract Ameliorates Memory Impairment and Beta amyloid (Aβ) clearance by LRP-1 Expression in the Hippocampus of a Rat model of Alzheimer’s Disease

Lee, Ju Won

doi:10.15188/kjopp.2016.10.30.5.347

Fructus Corni Officinalis water extract Ameliorates Memory Impairment and Beta amyloid (Aβ) clearance by LRP-1 Expression in the Hippocampus of a Rat model of Alzheimer’s Disease 원문보기

동의생리병리학회지 = Journal of physiology & pathology in Korean Medicine, v.30 no.5, 2016년, pp.347 - 354

Lee, Ju Won (Department of Korean Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)

Abstract ▼ AI-Helper

This study evaluated the effects of Fructus Corni Officinalis water extract (FCE) on congnitive impairment and Aβ clearance induced by beta amyloid Aβ (1-42) injection in the hippocampus of rat. Aβ (1-42) was injected into the hippocampus using a Hamilton syringe and micropump (5 ㎍/5 ㎕, 1 ㎕/min, each hippocampus bilaterally). FCE was administered orally once a day (100, 250, 500 mg/kg) for 4 weeks after the Aβ (1-42) injection. The acquisition of learning and retention of memory were tested using the Morris water maze. Aβ accumulation and Aβ clearance in the hippocampus were observed using immunostaining. Aβ (1-42) level in plasma was confirmed using enzyme-linked immunosorbent assay (ELISA). FCE significantly shortened the escape latencies during acquisition training trials. FCE significantly increased the number of target heading to the platform site and significantly shortened the time for the 1sttargetheadingduringtheretentiontesttrial.FCEsignificantlyattenuatedtheAβ accumulation in the hippocampus produced by Aβ (1-42) injection. FCE significantly increased LRP-1 expression around vessels in the hippocampus and Aβ (1-42) levels in plasma. The results suggest that FCE improved cognitive impairment by ameliorate Aβ clearance and Aβ accumulation in the hippocampus. FCE may be a beneficial herbal formulation in treating cognitive impairment including Alzheimer's disease.

주제어

AI 본문요약
AI-Helper

가설 설정

We confirmed Aβ clearance with LPR-1 immunostaining. We hypothesized that LRP-1 expression would decrease in the Aβ inj group, however our data demonstrated that change in LRP-1 expression varied by region. The AB inj group showed increased LRP-1 expression in CA1, but decreased expression in vessels.

대상 데이터

Male SD rats (280-300 g, Nara Biotechnology, Korea) were used for this study. All animal protocols were approved by the Ethics Committee for the Care and Use of Laboratory Animals at Kyung Hee University.

데이터처리

All data in this study are presented as means ± standard errors and evaluated using the Student’s t-test. A probability value of less than 0.
05 was used to indicate a significant difference. Differences between the escape latencies between groups in the acquisition trials were evaluated using one-way ANOVA. Following significant ANOVAs, multiple post hoc comparisons were performed using the Duncan test.
Differences between the escape latencies between groups in the acquisition trials were evaluated using one-way ANOVA. Following significant ANOVAs, multiple post hoc comparisons were performed using the Duncan test. All tests were performed using SPSS 20.

성능/효과

9% recovery), which is a known remedy for dementia. Our results suggest that FCE can improve memory deficits and cognitive impairment.
FCE ameliorated Aβ induced memory impairment and Aβ clearance deficits. Our results suggest that FCE is an effective alternative for treating AD.
From these findings, it appears that FCE may improve memory deficits and cognitive impairment in diverse conditions. Retention test showed, that the FCE 500 mg/kg group had 84.8% recovery compared to those in the control group in the escape latency. In particular FCE had a greater effect than Donepezil (76.
Aβ (1-42) accumulation in brain tissue was confirmed by immunostaining. The Aβ inj group displayed higher Aβ accumulation in the hippocampal CA1 and DG region than the control group, The FCE 500 mg/kg group displayed significantly lower Aβ accumulation in the hippocampal CA1 region than the Aβ inj group (Fig. 6).
The Morris water maze test, which assesses spatial reference memory and spatial working memory, was used to assess cognitive ability. The FCE groups exhibited an improvement in advanced learning memory and cognition, which became significant during the 3rd and 4th day of the 4 day training period (Fig 2B). FCE mainly benefited repeated learning and memory.
The AB inj group showed increased LRP-1 expression in CA1, but decreased expression in vessels. The likely explanation for these results is that LRP-1 colocalizes with Aβ in senile plaques33), which suggests a possible relationship between the decrease in vascular LRP-1 expression, the increase in neuronal LRP-1 expression, and Aβ induced toxicity. Therefore, there is a shift in LRP-1 immunoreactivity between neurons and vasculature.

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